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dot1l inhibitor epz004777  (MedChemExpress)
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MedChemExpress dot1l inhibitor epz004777
( A ) Extracellular acidification rate for C2C12 and MEFs cultured overnight in media containing 500 μM of either NaCl or Na2HB. N=4 independent assays, mean ± SD. Dunnett’s multiple comparisons test, * p<0.05. ( B ) OCR during mitochondrial stress test and maximum OCR for MEFs and C2C12s treated daily with 500 μM of Na2HB or NaCl supplemented media for 3 days with assays run on day 4. N=4 independent assays, Sidak’s multiple comparisons test, ** p<0.01, * p<0.05. ( C ) Representative western blot and quantification for histones isolated from MEFs treated for 3 days with 500 μM of Na2HB or NaCl, with or without the <t>DOT1L</t> methyltransferase inhibitor <t>EPZ004777.</t> Amounts of protein loaded are indicated above each blot. ( D ) Representative western blots and quantification using histones isolated from C2C12 cells treated for 3 days with 2HB or NaCl control at indicated concentrations. EPZ004777 was used as a positive control DOT1L inhibitor. ( E ) Representative western blot using OXPHOS antibody cocktail from C2C12 (left) and MEF (right) cell lysates following 3 days of treatment with NaCl (Cl), Na2HB (HB), DMSO ( D ), or ethyl 2HB (Et). ‘+’ indicates positive control sample. Amount of protein loaded in each well is indicated above. Total protein staining and histone methylation markers displayed. For all western blots, total protein for each well was detected using REVERT total protein stain. ( F ) Quantification from two independent assays of OXPHOS antibody cocktail western blots using lysates from MEFs treated with 2HB for three days. No observed change to any respiratory chain complex from 2HB treatment is suggested. Figure 5—figure supplement 1—source data 1. Raw data for . Figure 5—figure supplement 1—source data 2. Labeled western blots. Figure 5—figure supplement 1—source data 3. Unlabeled western blots.
Dot1l Inhibitor Epz004777, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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( A ) Extracellular acidification rate for C2C12 and MEFs cultured overnight in media containing 500 μM of either NaCl or Na2HB. N=4 independent assays, mean ± SD. Dunnett’s multiple comparisons test, * p<0.05. ( B ) OCR during mitochondrial stress test and maximum OCR for MEFs and C2C12s treated daily with 500 μM of Na2HB or NaCl supplemented media for 3 days with assays run on day 4. N=4 independent assays, Sidak’s multiple comparisons test, ** p<0.01, * p<0.05. ( C ) Representative western blot and quantification for histones isolated from MEFs treated for 3 days with 500 μM of Na2HB or NaCl, with or without the DOT1L methyltransferase inhibitor EPZ004777. Amounts of protein loaded are indicated above each blot. ( D ) Representative western blots and quantification using histones isolated from C2C12 cells treated for 3 days with 2HB or NaCl control at indicated concentrations. EPZ004777 was used as a positive control DOT1L inhibitor. ( E ) Representative western blot using OXPHOS antibody cocktail from C2C12 (left) and MEF (right) cell lysates following 3 days of treatment with NaCl (Cl), Na2HB (HB), DMSO ( D ), or ethyl 2HB (Et). ‘+’ indicates positive control sample. Amount of protein loaded in each well is indicated above. Total protein staining and histone methylation markers displayed. For all western blots, total protein for each well was detected using REVERT total protein stain. ( F ) Quantification from two independent assays of OXPHOS antibody cocktail western blots using lysates from MEFs treated with 2HB for three days. No observed change to any respiratory chain complex from 2HB treatment is suggested. Figure 5—figure supplement 1—source data 1. Raw data for . Figure 5—figure supplement 1—source data 2. Labeled western blots. Figure 5—figure supplement 1—source data 3. Unlabeled western blots.

Journal: eLife

Article Title: A 2-hydroxybutyrate-mediated feedback loop regulates muscular fatigue

doi: 10.7554/eLife.92707

Figure Lengend Snippet: ( A ) Extracellular acidification rate for C2C12 and MEFs cultured overnight in media containing 500 μM of either NaCl or Na2HB. N=4 independent assays, mean ± SD. Dunnett’s multiple comparisons test, * p<0.05. ( B ) OCR during mitochondrial stress test and maximum OCR for MEFs and C2C12s treated daily with 500 μM of Na2HB or NaCl supplemented media for 3 days with assays run on day 4. N=4 independent assays, Sidak’s multiple comparisons test, ** p<0.01, * p<0.05. ( C ) Representative western blot and quantification for histones isolated from MEFs treated for 3 days with 500 μM of Na2HB or NaCl, with or without the DOT1L methyltransferase inhibitor EPZ004777. Amounts of protein loaded are indicated above each blot. ( D ) Representative western blots and quantification using histones isolated from C2C12 cells treated for 3 days with 2HB or NaCl control at indicated concentrations. EPZ004777 was used as a positive control DOT1L inhibitor. ( E ) Representative western blot using OXPHOS antibody cocktail from C2C12 (left) and MEF (right) cell lysates following 3 days of treatment with NaCl (Cl), Na2HB (HB), DMSO ( D ), or ethyl 2HB (Et). ‘+’ indicates positive control sample. Amount of protein loaded in each well is indicated above. Total protein staining and histone methylation markers displayed. For all western blots, total protein for each well was detected using REVERT total protein stain. ( F ) Quantification from two independent assays of OXPHOS antibody cocktail western blots using lysates from MEFs treated with 2HB for three days. No observed change to any respiratory chain complex from 2HB treatment is suggested. Figure 5—figure supplement 1—source data 1. Raw data for . Figure 5—figure supplement 1—source data 2. Labeled western blots. Figure 5—figure supplement 1—source data 3. Unlabeled western blots.

Article Snippet: Aminooxyacetic acid (AOA; Sigma) was used as an inhibitor of aminotransferases, and the DOT1L inhibitor EPZ004777 (MedChemExpress).

Techniques: Cell Culture, Western Blot, Isolation, Control, Positive Control, Staining, Methylation, Labeling