HY-142160 Search Results


gne-9815  (MedChemExpress)
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GNE-9815 (compound 7) is a highly selective, pan-RAF inhibitor with good oral bioavailability. GNE-9815 exhibits Ki values of 0.062 and 0.19 nM for CRAF and BRAF, respectively. GNE-9815 combines with MEK inhibitor Cobimetinib (HY-13064) shows
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abd957  (MedChemExpress)
94
MedChemExpress abd957
Fig. 1. Determination of palmitoylated Nrf2 in gastric carcinoma cell. A, Human NCI–N87 cells were treated with gradually increasing doses of 2-bromopalmitate (2-BP) for 24 h, and subjected to immunoblotting detection of Nrf2 and Gapdh antibodies (n = 4 per group). B, NCI–N87 cells were treated with 60 μM 2-BP for 0, 6, 12 and 24 h and then subjected to immunoblotting with Nrf2 and Gapdh antibodies (n = 4 per group). C, D, NCI–N87 cells were incubated with 0, 3 or 6 μM palmostatin B (Palm B) (C) palmostatin M (Palm M) (D), an inhibitor of depalmitoylase enzymes, for 24 h, and subjected to immunoblotting analysis with Nrf2 and Gapdh antibodies (n = 4 per group). E, NCI–N87 cells were treated with 60 μM 2-BP, 1 μM <t>ABD957,</t> 6 μM Palm B/Palm M for 24 h. The fixed cells coverslips were subjected to immunofluorescent staining with Nrf2 (green) and DAPI (blue). The bar graph indicating Nrf2 fluorescence intensity in the indicated group (n = 5 images per group; P < 0.05 vs. Control). Scale bars, 10 μm. F, NCI–N87 cells were pre-incubated with DMSO, 2-BP, ABD957, Palm B or Palm M as a baseline, then treated with cycloheximide (CHX). The collected cell lysates were subjected to immunoblotting detection with Nrf2 and Gapdh antibodies. The right curve graph showing the relative Nrf2 remaining ratio in the indicated time point and cor responding half-time of Nrf2 protein (n = 4 per group). G, Schematic diagram of the Click-iT assay used for Nrf2 palmitoylation analysis. Cells were incubated with 100 μM Click-iT palmitic acid-Azides for 8 h, and lysates were subjected to Click-iT detection, followed by western blotting analysis with Nrf2 antibody. The right western blotting show the Nrf2 expression in the indicated group. Data are expressed as mean ± SEM. The relevant experiments presented in this part were per formed independently at least three times. Significance was determined by one-way analysis of variance (ANOVA) followed by Dunnett’s multiple comparisons test analysis (E). The P value less than 0.05 was considered as significant difference. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)
Abd957, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Fig. 1. Determination of palmitoylated Nrf2 in gastric carcinoma cell. A, Human NCI–N87 cells were treated with gradually increasing doses of 2-bromopalmitate (2-BP) for 24 h, and subjected to immunoblotting detection of Nrf2 and Gapdh antibodies (n = 4 per group). B, NCI–N87 cells were treated with 60 μM 2-BP for 0, 6, 12 and 24 h and then subjected to immunoblotting with Nrf2 and Gapdh antibodies (n = 4 per group). C, D, NCI–N87 cells were incubated with 0, 3 or 6 μM palmostatin B (Palm B) (C) palmostatin M (Palm M) (D), an inhibitor of depalmitoylase enzymes, for 24 h, and subjected to immunoblotting analysis with Nrf2 and Gapdh antibodies (n = 4 per group). E, NCI–N87 cells were treated with 60 μM 2-BP, 1 μM ABD957, 6 μM Palm B/Palm M for 24 h. The fixed cells coverslips were subjected to immunofluorescent staining with Nrf2 (green) and DAPI (blue). The bar graph indicating Nrf2 fluorescence intensity in the indicated group (n = 5 images per group; P < 0.05 vs. Control). Scale bars, 10 μm. F, NCI–N87 cells were pre-incubated with DMSO, 2-BP, ABD957, Palm B or Palm M as a baseline, then treated with cycloheximide (CHX). The collected cell lysates were subjected to immunoblotting detection with Nrf2 and Gapdh antibodies. The right curve graph showing the relative Nrf2 remaining ratio in the indicated time point and cor responding half-time of Nrf2 protein (n = 4 per group). G, Schematic diagram of the Click-iT assay used for Nrf2 palmitoylation analysis. Cells were incubated with 100 μM Click-iT palmitic acid-Azides for 8 h, and lysates were subjected to Click-iT detection, followed by western blotting analysis with Nrf2 antibody. The right western blotting show the Nrf2 expression in the indicated group. Data are expressed as mean ± SEM. The relevant experiments presented in this part were per formed independently at least three times. Significance was determined by one-way analysis of variance (ANOVA) followed by Dunnett’s multiple comparisons test analysis (E). The P value less than 0.05 was considered as significant difference. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)

Journal: Redox biology

Article Title: Acyltransferase zinc finger DHHC-type containing 2 aggravates gastric carcinoma growth by targeting Nrf2 signaling: A mechanism-based multicombination bionic nano-drug therapy.

doi: 10.1016/j.redox.2024.103051

Figure Lengend Snippet: Fig. 1. Determination of palmitoylated Nrf2 in gastric carcinoma cell. A, Human NCI–N87 cells were treated with gradually increasing doses of 2-bromopalmitate (2-BP) for 24 h, and subjected to immunoblotting detection of Nrf2 and Gapdh antibodies (n = 4 per group). B, NCI–N87 cells were treated with 60 μM 2-BP for 0, 6, 12 and 24 h and then subjected to immunoblotting with Nrf2 and Gapdh antibodies (n = 4 per group). C, D, NCI–N87 cells were incubated with 0, 3 or 6 μM palmostatin B (Palm B) (C) palmostatin M (Palm M) (D), an inhibitor of depalmitoylase enzymes, for 24 h, and subjected to immunoblotting analysis with Nrf2 and Gapdh antibodies (n = 4 per group). E, NCI–N87 cells were treated with 60 μM 2-BP, 1 μM ABD957, 6 μM Palm B/Palm M for 24 h. The fixed cells coverslips were subjected to immunofluorescent staining with Nrf2 (green) and DAPI (blue). The bar graph indicating Nrf2 fluorescence intensity in the indicated group (n = 5 images per group; P < 0.05 vs. Control). Scale bars, 10 μm. F, NCI–N87 cells were pre-incubated with DMSO, 2-BP, ABD957, Palm B or Palm M as a baseline, then treated with cycloheximide (CHX). The collected cell lysates were subjected to immunoblotting detection with Nrf2 and Gapdh antibodies. The right curve graph showing the relative Nrf2 remaining ratio in the indicated time point and cor responding half-time of Nrf2 protein (n = 4 per group). G, Schematic diagram of the Click-iT assay used for Nrf2 palmitoylation analysis. Cells were incubated with 100 μM Click-iT palmitic acid-Azides for 8 h, and lysates were subjected to Click-iT detection, followed by western blotting analysis with Nrf2 antibody. The right western blotting show the Nrf2 expression in the indicated group. Data are expressed as mean ± SEM. The relevant experiments presented in this part were per formed independently at least three times. Significance was determined by one-way analysis of variance (ANOVA) followed by Dunnett’s multiple comparisons test analysis (E). The P value less than 0.05 was considered as significant difference. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)

Article Snippet: The MG132 (Cat#: HY-13259), cycloheximide (Cat#: HY-12320), dimethylsulfoxide (Cat#: HY-Y0320), ABD957 (Cat#: HY-142161), 3-methyladenine (Cat#: HY-19312), bortezomib (Cat#: HY-10227) and chloroquine (Cat#: HY-17589 A) were purchased from MedChemExpress (MCE), Shanghai, China.

Techniques: Western Blot, Incubation, Staining, Fluorescence, Control, Expressing