HY-13780 Search Results


vinblastine sulfate  (MedChemExpress)
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MedChemExpress vinblastine sulfate
Effect of <t>vinblastine</t> on the viability of H9c2 cells. ( a ) H9c2 cells were treated with DMSO, daunorubicin (Da), doxorubicin (Do), vinblastine (V), cisplatin (Cis) and 5-fluorouracil (5-Fu) at the indicated concentrations for 48 h and were observed under a microscope (200 ×). ( b ) H9c2 cells were pre-treated with 10 μM SB203580 (SB), 5 μM BV6, 50 μM ZVAD-fmk or 5 mM 3-MA for 1 h and treated with DMSO or the indicated concentrations of vinblastine for 48 h. Cell viability was measured using the WST-8 assay. Data represent mean ± SD from three independent experiments. * p < 0.05, ** p < 0.01, *** p < 0.001. ( c ) H9c2 cells were treated with DMSO or various concentrations of vinblastine for 48 h and cell viability was measured by WST-8 assay. Data represent mean ± SD from three independent experiments. * p < 0.05, ** p < 0.01 vs. DMSO-treated group. ( d ) H9c2 cells were treated with DMSO or vinblastine (0.01, 0.03, 0.3 or 3 μM) for 48 h; cells were subjected to propidium iodide and Annexin V-FITC staining and analyzed by flow cytometry
Vinblastine Sulfate, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Effect of vinblastine on the viability of H9c2 cells. ( a ) H9c2 cells were treated with DMSO, daunorubicin (Da), doxorubicin (Do), vinblastine (V), cisplatin (Cis) and 5-fluorouracil (5-Fu) at the indicated concentrations for 48 h and were observed under a microscope (200 ×). ( b ) H9c2 cells were pre-treated with 10 μM SB203580 (SB), 5 μM BV6, 50 μM ZVAD-fmk or 5 mM 3-MA for 1 h and treated with DMSO or the indicated concentrations of vinblastine for 48 h. Cell viability was measured using the WST-8 assay. Data represent mean ± SD from three independent experiments. * p < 0.05, ** p < 0.01, *** p < 0.001. ( c ) H9c2 cells were treated with DMSO or various concentrations of vinblastine for 48 h and cell viability was measured by WST-8 assay. Data represent mean ± SD from three independent experiments. * p < 0.05, ** p < 0.01 vs. DMSO-treated group. ( d ) H9c2 cells were treated with DMSO or vinblastine (0.01, 0.03, 0.3 or 3 μM) for 48 h; cells were subjected to propidium iodide and Annexin V-FITC staining and analyzed by flow cytometry

Journal: Molecular and Cellular Biochemistry

Article Title: RIP1/RIP3/MLKL-mediated necroptosis contributes to vinblastine-induced myocardial damage

doi: 10.1007/s11010-020-03985-3

Figure Lengend Snippet: Effect of vinblastine on the viability of H9c2 cells. ( a ) H9c2 cells were treated with DMSO, daunorubicin (Da), doxorubicin (Do), vinblastine (V), cisplatin (Cis) and 5-fluorouracil (5-Fu) at the indicated concentrations for 48 h and were observed under a microscope (200 ×). ( b ) H9c2 cells were pre-treated with 10 μM SB203580 (SB), 5 μM BV6, 50 μM ZVAD-fmk or 5 mM 3-MA for 1 h and treated with DMSO or the indicated concentrations of vinblastine for 48 h. Cell viability was measured using the WST-8 assay. Data represent mean ± SD from three independent experiments. * p < 0.05, ** p < 0.01, *** p < 0.001. ( c ) H9c2 cells were treated with DMSO or various concentrations of vinblastine for 48 h and cell viability was measured by WST-8 assay. Data represent mean ± SD from three independent experiments. * p < 0.05, ** p < 0.01 vs. DMSO-treated group. ( d ) H9c2 cells were treated with DMSO or vinblastine (0.01, 0.03, 0.3 or 3 μM) for 48 h; cells were subjected to propidium iodide and Annexin V-FITC staining and analyzed by flow cytometry

Article Snippet: Vinblastine sulfate (>99% purity, cat#HY-13780), daunorubicin (cat#HY-13062), doxorubicin (cat#HY-15142) and necrostatin-1 (cat#HY-15760) were purchased from MedChemExpress (New Jersey, USA).

Techniques: Microscopy, Staining, Flow Cytometry

Vinblastine causes rat myocardial injury in vivo. ( a ) SD rats were randomly divided into three groups and treated as described in Materials and Methods. At the treatment endpoint, rats were sacrificed and hearts were removed and photographed. ( b ) Hearts of rats were weighed and recorded. ** p < 0.01, *** p < 0.001. ( c ) Overall survival rates of SD rats with saline ( n = 7), VBL ( n = 14) or VBL+ Necrostatin-1 (Nec-1) ( n = 12) were analyzed with Kaplan–Meier method by log-rank test. * p < 0.05, ** p < 0.01. ( d ) H&E staining of heart tissues from the rats of saline-, VBL- and VBL + Nec-1-treated groups. Figures were representative of two independent experiments

Journal: Molecular and Cellular Biochemistry

Article Title: RIP1/RIP3/MLKL-mediated necroptosis contributes to vinblastine-induced myocardial damage

doi: 10.1007/s11010-020-03985-3

Figure Lengend Snippet: Vinblastine causes rat myocardial injury in vivo. ( a ) SD rats were randomly divided into three groups and treated as described in Materials and Methods. At the treatment endpoint, rats were sacrificed and hearts were removed and photographed. ( b ) Hearts of rats were weighed and recorded. ** p < 0.01, *** p < 0.001. ( c ) Overall survival rates of SD rats with saline ( n = 7), VBL ( n = 14) or VBL+ Necrostatin-1 (Nec-1) ( n = 12) were analyzed with Kaplan–Meier method by log-rank test. * p < 0.05, ** p < 0.01. ( d ) H&E staining of heart tissues from the rats of saline-, VBL- and VBL + Nec-1-treated groups. Figures were representative of two independent experiments

Article Snippet: Vinblastine sulfate (>99% purity, cat#HY-13780), daunorubicin (cat#HY-13062), doxorubicin (cat#HY-15142) and necrostatin-1 (cat#HY-15760) were purchased from MedChemExpress (New Jersey, USA).

Techniques: In Vivo, Saline, Staining

Necroptosis is involved in vinblastine-induced H9c2 cell death. The inhibitors of necroptosis ameliorate the VBL-induced cardiomyocytes injury. H9c2 cells were pre-treated with 50 μM Necrostatin-1 (Nec-1) or 2.5 μM Necrosulfonamide (NSA) for 1 h and then treated with DMSO or the indicated concentrations of VBL for 48 h. Cell viability was measured using the WST-8 assay. Data represent mean ± SD from three independent experiments. * p < 0.05, ** p < 0.01, *** p < 0.001

Journal: Molecular and Cellular Biochemistry

Article Title: RIP1/RIP3/MLKL-mediated necroptosis contributes to vinblastine-induced myocardial damage

doi: 10.1007/s11010-020-03985-3

Figure Lengend Snippet: Necroptosis is involved in vinblastine-induced H9c2 cell death. The inhibitors of necroptosis ameliorate the VBL-induced cardiomyocytes injury. H9c2 cells were pre-treated with 50 μM Necrostatin-1 (Nec-1) or 2.5 μM Necrosulfonamide (NSA) for 1 h and then treated with DMSO or the indicated concentrations of VBL for 48 h. Cell viability was measured using the WST-8 assay. Data represent mean ± SD from three independent experiments. * p < 0.05, ** p < 0.01, *** p < 0.001

Article Snippet: Vinblastine sulfate (>99% purity, cat#HY-13780), daunorubicin (cat#HY-13062), doxorubicin (cat#HY-15142) and necrostatin-1 (cat#HY-15760) were purchased from MedChemExpress (New Jersey, USA).

Techniques:

Effect of vinblastine on the expression of necroptosis-related proteins in H9c2 cells. ( a ) H9c2 cells were treated with DMSO or the indicated concentrations of vinblastine for 48 h. After treatment, the attached and floating cells were harvested. Expression of the indicated proteins was analyzed by Western blotting with specific antibodies. GAPDH was used as a loading control. ( b ) The relative protein level of each protein was shown. ( c ) H9c2 cells were pre-treated with the indicated necroptosis inhibitors for 1 h and then treated with DMSO or 0.3 μM vinblastine for 48 h. After treatment, the attached and floating cells were harvested. Expression of the indicated proteins was analyzed by Western blotting with specific antibodies. GAPDH was used as a loading control. ( d ) The relative level of each protein was shown. Data are representative of three independent experiments

Journal: Molecular and Cellular Biochemistry

Article Title: RIP1/RIP3/MLKL-mediated necroptosis contributes to vinblastine-induced myocardial damage

doi: 10.1007/s11010-020-03985-3

Figure Lengend Snippet: Effect of vinblastine on the expression of necroptosis-related proteins in H9c2 cells. ( a ) H9c2 cells were treated with DMSO or the indicated concentrations of vinblastine for 48 h. After treatment, the attached and floating cells were harvested. Expression of the indicated proteins was analyzed by Western blotting with specific antibodies. GAPDH was used as a loading control. ( b ) The relative protein level of each protein was shown. ( c ) H9c2 cells were pre-treated with the indicated necroptosis inhibitors for 1 h and then treated with DMSO or 0.3 μM vinblastine for 48 h. After treatment, the attached and floating cells were harvested. Expression of the indicated proteins was analyzed by Western blotting with specific antibodies. GAPDH was used as a loading control. ( d ) The relative level of each protein was shown. Data are representative of three independent experiments

Article Snippet: Vinblastine sulfate (>99% purity, cat#HY-13780), daunorubicin (cat#HY-13062), doxorubicin (cat#HY-15142) and necrostatin-1 (cat#HY-15760) were purchased from MedChemExpress (New Jersey, USA).

Techniques: Expressing, Western Blot, Control

Effect of vinblastine on the expression of necroptosis-related proteins in primary neonatal rat ventricular myocytes. ( a ) Primary neonatal rat ventricular myocytes were pre-treated with indicated inhibitors for 1 h and treated with DMSO or indicated concentrations of vinblastine for 48 h. Expression of the indicated proteins was analyzed by Western blotting with specific antibodies. GAPDH was used as a loading control. ( b ) The relative protein level of each protein was shown. Data are representative of two independent experiments

Journal: Molecular and Cellular Biochemistry

Article Title: RIP1/RIP3/MLKL-mediated necroptosis contributes to vinblastine-induced myocardial damage

doi: 10.1007/s11010-020-03985-3

Figure Lengend Snippet: Effect of vinblastine on the expression of necroptosis-related proteins in primary neonatal rat ventricular myocytes. ( a ) Primary neonatal rat ventricular myocytes were pre-treated with indicated inhibitors for 1 h and treated with DMSO or indicated concentrations of vinblastine for 48 h. Expression of the indicated proteins was analyzed by Western blotting with specific antibodies. GAPDH was used as a loading control. ( b ) The relative protein level of each protein was shown. Data are representative of two independent experiments

Article Snippet: Vinblastine sulfate (>99% purity, cat#HY-13780), daunorubicin (cat#HY-13062), doxorubicin (cat#HY-15142) and necrostatin-1 (cat#HY-15760) were purchased from MedChemExpress (New Jersey, USA).

Techniques: Expressing, Western Blot, Control

Vinblastine promotes the endogenous RIP1 and RIP3 interaction. ( a ) H9c2 cells were cultured with DMSO or the indicated concentrations of vinblastine for 48 h. After treatment, cells were lysed and immunoprecipitated with a RIP3 or a normal IgG antibody. The immune complexes and input were analyzed by immunoblotting with a RIP1 antibody. ( b ) The data were normalized to input and shown as mean ± SD of two separate experiments. * p < 0.05, significant difference compared with the DMSO-treated group

Journal: Molecular and Cellular Biochemistry

Article Title: RIP1/RIP3/MLKL-mediated necroptosis contributes to vinblastine-induced myocardial damage

doi: 10.1007/s11010-020-03985-3

Figure Lengend Snippet: Vinblastine promotes the endogenous RIP1 and RIP3 interaction. ( a ) H9c2 cells were cultured with DMSO or the indicated concentrations of vinblastine for 48 h. After treatment, cells were lysed and immunoprecipitated with a RIP3 or a normal IgG antibody. The immune complexes and input were analyzed by immunoblotting with a RIP1 antibody. ( b ) The data were normalized to input and shown as mean ± SD of two separate experiments. * p < 0.05, significant difference compared with the DMSO-treated group

Article Snippet: Vinblastine sulfate (>99% purity, cat#HY-13780), daunorubicin (cat#HY-13062), doxorubicin (cat#HY-15142) and necrostatin-1 (cat#HY-15760) were purchased from MedChemExpress (New Jersey, USA).

Techniques: Cell Culture, Immunoprecipitation, Western Blot

Effect of vinblastine on the expression of necroptosis-related proteins in SD rats. ( a , b ) SD rats were randomly divided into three groups and treated according to the protocol described in Materials and Methods. At the treatment endpoint, rats were sacrificed and left ventricle tissues were collected and lysed. Expression of the indicated proteins was analyzed by Western blotting with specific antibodies. GAPDH was used as a loading control. ( c ) Immunohistochemical staining examination of RIP1, RIP3 and MLKL in rat left ventricle tissues from the saline-, VBL- or VBL + Nec-1-treated group. All panels were shown as the indicated magnification (100×). And the density of each protein was shown. * p < 0.05, ** p < 0.01, *** p < 0.001. Data were representative of two independent experiments

Journal: Molecular and Cellular Biochemistry

Article Title: RIP1/RIP3/MLKL-mediated necroptosis contributes to vinblastine-induced myocardial damage

doi: 10.1007/s11010-020-03985-3

Figure Lengend Snippet: Effect of vinblastine on the expression of necroptosis-related proteins in SD rats. ( a , b ) SD rats were randomly divided into three groups and treated according to the protocol described in Materials and Methods. At the treatment endpoint, rats were sacrificed and left ventricle tissues were collected and lysed. Expression of the indicated proteins was analyzed by Western blotting with specific antibodies. GAPDH was used as a loading control. ( c ) Immunohistochemical staining examination of RIP1, RIP3 and MLKL in rat left ventricle tissues from the saline-, VBL- or VBL + Nec-1-treated group. All panels were shown as the indicated magnification (100×). And the density of each protein was shown. * p < 0.05, ** p < 0.01, *** p < 0.001. Data were representative of two independent experiments

Article Snippet: Vinblastine sulfate (>99% purity, cat#HY-13780), daunorubicin (cat#HY-13062), doxorubicin (cat#HY-15142) and necrostatin-1 (cat#HY-15760) were purchased from MedChemExpress (New Jersey, USA).

Techniques: Expressing, Western Blot, Control, Immunohistochemical staining, Staining, Saline