HY-110184 Search Results


pk-thpp  (MedChemExpress)
N/A
PK-THPP is a potent and brain-penetrant TWIK-related acid-sensitive K+ ion channel (K2P9.1 or TASK-3) blocker with IC50s of 35 nM and 300 nM for TASK-3 and TASK-1, respectively. PK-THPP shows no or less activity against
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pregnenolone monosulfate sodium  (MedChemExpress)
93
MedChemExpress pregnenolone monosulfate sodium
( A ) Time course (left) and I-V relationship (right) of a whole-cell currents induced by CIM0216 (5 μM) in CHO cells expressing TRPM3 (top) or TRPM3 + Copine-6 (bottom). ( B ) Voltage dependence of CIM0216-induced currents in TRPM3-expressing CHO cells with (red) and without (black) Copine-6 co-expression. Currents were induced by voltage ramps from −150 to +150 mV in 900 ms. Current-voltage relationships normalized to I TRPM3 at +150 mV are shown. TRPM3, n = 12; TRPM3 + Copine-6, n = 19. ( C ) Comparison of 5 μM CIM0216-induced current densities in CHO cells expressing TRPM3 ( NM_177341.4 /γ3), either alone or together with Copine-6 at −80 or +80 mV. TRPM3, n = 12; TRPM3 + Copine-6, n = 19; Data are shown as mean ± SEM; −80 mV, *** P < 0.001; +80 mV, ** P = 0.003 (two-tailed independent t test). ( D ) Current traces obtained with a voltage step protocol (inset on the bottom-left) in CHO cells transfected with TRPM3 alone (black) or co-transfected with Copine-6 (red) after stimulation by 5 μM CIM0216. ( E ) Voltage dependence of CIM0216-induced currents in TRPM3-expressing CHO cells with (red) and without (black) Copine-6 co-expression. Current-voltage relationships normalized to I TRPM3 at +200 mV are shown. TRPM3, n = 13; TRPM3 + Copine-6, n = 19; Data are shown as mean ± SEM; * P = 0.0188 (two-way ANOVA followed by Bonferroni multiple comparisons test). ( F ) Concentration-response curves for <t>Pregnenolone</t> <t>monosulfate</t> sodium (PregS) in CHO cells transfected with TRPM3 alone (black) or co-transfected with Copine-6 (red) at −80 mV (left) and +80 mV (right); recordings as these shown in ( A ). The EC 50 values are shown next to the curves. Current densities (pA/pF) represent the peak values of the PregS-induced currents. The number of recordings, n (from low to high concentration of PregS, respectively): 22, 18, 17, 20 22, and 20 for cells expressing TRPM3 alone (black); 20, 20, 20, 20, 19 and 18 for cells expressing TRPM3 and Copine-6 (red). Data are shown as mean ± SEM. All curves were fitted with Four-Parameter Logistic function. ( G ) Similar to ( F ), but CIM0216 concentration-response curves are plotted. The number of recordings, n (from low to high concentration of CIM0216, respectively): 17, 16, 16, 17 15, and 12 for cells expressing TRPM3 alone (black); 17, 17, 17, 14, 13 and 13 for cells expressing TRPM3 and Copine-6 (red). Data are shown as mean ± SEM. ( H ) Comparison of 5 μM CIM0216-induced current densities in CHO cells expressing TRPM3 splice variants ( NM_001035242.1 /α2 or NM_001035240.2 /α3), either alone or together with Copine-6 at −80 or +80 mV. NM_001035242.1 /α2: TRPM3, n = 16; TRPM3 + Copine-6, n = 22; NM_001035240.2 /α3: TRPM3, n = 25; TRPM3 + Copine-6, n = 28. Data are shown as mean ± SEM. NM_001035242.1 /α2: -80 mV, ** P = 0.002; +80 mV, *** P < 0.0001. NM_001035240.2 /α3: −80 mV, ** P = 0.003; +80 mV, ** P = 0.0003 (two-tailed independent Student’s t-test). .
Pregnenolone Monosulfate Sodium, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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wrn helicase inhibitor nsc 617145  (MedChemExpress)
94
MedChemExpress wrn helicase inhibitor nsc 617145
( A ) Time course (left) and I-V relationship (right) of a whole-cell currents induced by CIM0216 (5 μM) in CHO cells expressing TRPM3 (top) or TRPM3 + Copine-6 (bottom). ( B ) Voltage dependence of CIM0216-induced currents in TRPM3-expressing CHO cells with (red) and without (black) Copine-6 co-expression. Currents were induced by voltage ramps from −150 to +150 mV in 900 ms. Current-voltage relationships normalized to I TRPM3 at +150 mV are shown. TRPM3, n = 12; TRPM3 + Copine-6, n = 19. ( C ) Comparison of 5 μM CIM0216-induced current densities in CHO cells expressing TRPM3 ( NM_177341.4 /γ3), either alone or together with Copine-6 at −80 or +80 mV. TRPM3, n = 12; TRPM3 + Copine-6, n = 19; Data are shown as mean ± SEM; −80 mV, *** P < 0.001; +80 mV, ** P = 0.003 (two-tailed independent t test). ( D ) Current traces obtained with a voltage step protocol (inset on the bottom-left) in CHO cells transfected with TRPM3 alone (black) or co-transfected with Copine-6 (red) after stimulation by 5 μM CIM0216. ( E ) Voltage dependence of CIM0216-induced currents in TRPM3-expressing CHO cells with (red) and without (black) Copine-6 co-expression. Current-voltage relationships normalized to I TRPM3 at +200 mV are shown. TRPM3, n = 13; TRPM3 + Copine-6, n = 19; Data are shown as mean ± SEM; * P = 0.0188 (two-way ANOVA followed by Bonferroni multiple comparisons test). ( F ) Concentration-response curves for <t>Pregnenolone</t> <t>monosulfate</t> sodium (PregS) in CHO cells transfected with TRPM3 alone (black) or co-transfected with Copine-6 (red) at −80 mV (left) and +80 mV (right); recordings as these shown in ( A ). The EC 50 values are shown next to the curves. Current densities (pA/pF) represent the peak values of the PregS-induced currents. The number of recordings, n (from low to high concentration of PregS, respectively): 22, 18, 17, 20 22, and 20 for cells expressing TRPM3 alone (black); 20, 20, 20, 20, 19 and 18 for cells expressing TRPM3 and Copine-6 (red). Data are shown as mean ± SEM. All curves were fitted with Four-Parameter Logistic function. ( G ) Similar to ( F ), but CIM0216 concentration-response curves are plotted. The number of recordings, n (from low to high concentration of CIM0216, respectively): 17, 16, 16, 17 15, and 12 for cells expressing TRPM3 alone (black); 17, 17, 17, 14, 13 and 13 for cells expressing TRPM3 and Copine-6 (red). Data are shown as mean ± SEM. ( H ) Comparison of 5 μM CIM0216-induced current densities in CHO cells expressing TRPM3 splice variants ( NM_001035242.1 /α2 or NM_001035240.2 /α3), either alone or together with Copine-6 at −80 or +80 mV. NM_001035242.1 /α2: TRPM3, n = 16; TRPM3 + Copine-6, n = 22; NM_001035240.2 /α3: TRPM3, n = 25; TRPM3 + Copine-6, n = 28. Data are shown as mean ± SEM. NM_001035242.1 /α2: -80 mV, ** P = 0.002; +80 mV, *** P < 0.0001. NM_001035240.2 /α3: −80 mV, ** P = 0.003; +80 mV, ** P = 0.0003 (two-tailed independent Student’s t-test). .
Wrn Helicase Inhibitor Nsc 617145, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/HY-110184/NSC+617145/pmc13006356-58-1-9
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s1413 sp141 medchemexpress  (MedChemExpress)
94
MedChemExpress s1413 sp141 medchemexpress
( A ) Time course (left) and I-V relationship (right) of a whole-cell currents induced by CIM0216 (5 μM) in CHO cells expressing TRPM3 (top) or TRPM3 + Copine-6 (bottom). ( B ) Voltage dependence of CIM0216-induced currents in TRPM3-expressing CHO cells with (red) and without (black) Copine-6 co-expression. Currents were induced by voltage ramps from −150 to +150 mV in 900 ms. Current-voltage relationships normalized to I TRPM3 at +150 mV are shown. TRPM3, n = 12; TRPM3 + Copine-6, n = 19. ( C ) Comparison of 5 μM CIM0216-induced current densities in CHO cells expressing TRPM3 ( NM_177341.4 /γ3), either alone or together with Copine-6 at −80 or +80 mV. TRPM3, n = 12; TRPM3 + Copine-6, n = 19; Data are shown as mean ± SEM; −80 mV, *** P < 0.001; +80 mV, ** P = 0.003 (two-tailed independent t test). ( D ) Current traces obtained with a voltage step protocol (inset on the bottom-left) in CHO cells transfected with TRPM3 alone (black) or co-transfected with Copine-6 (red) after stimulation by 5 μM CIM0216. ( E ) Voltage dependence of CIM0216-induced currents in TRPM3-expressing CHO cells with (red) and without (black) Copine-6 co-expression. Current-voltage relationships normalized to I TRPM3 at +200 mV are shown. TRPM3, n = 13; TRPM3 + Copine-6, n = 19; Data are shown as mean ± SEM; * P = 0.0188 (two-way ANOVA followed by Bonferroni multiple comparisons test). ( F ) Concentration-response curves for <t>Pregnenolone</t> <t>monosulfate</t> sodium (PregS) in CHO cells transfected with TRPM3 alone (black) or co-transfected with Copine-6 (red) at −80 mV (left) and +80 mV (right); recordings as these shown in ( A ). The EC 50 values are shown next to the curves. Current densities (pA/pF) represent the peak values of the PregS-induced currents. The number of recordings, n (from low to high concentration of PregS, respectively): 22, 18, 17, 20 22, and 20 for cells expressing TRPM3 alone (black); 20, 20, 20, 20, 19 and 18 for cells expressing TRPM3 and Copine-6 (red). Data are shown as mean ± SEM. All curves were fitted with Four-Parameter Logistic function. ( G ) Similar to ( F ), but CIM0216 concentration-response curves are plotted. The number of recordings, n (from low to high concentration of CIM0216, respectively): 17, 16, 16, 17 15, and 12 for cells expressing TRPM3 alone (black); 17, 17, 17, 14, 13 and 13 for cells expressing TRPM3 and Copine-6 (red). Data are shown as mean ± SEM. ( H ) Comparison of 5 μM CIM0216-induced current densities in CHO cells expressing TRPM3 splice variants ( NM_001035242.1 /α2 or NM_001035240.2 /α3), either alone or together with Copine-6 at −80 or +80 mV. NM_001035242.1 /α2: TRPM3, n = 16; TRPM3 + Copine-6, n = 22; NM_001035240.2 /α3: TRPM3, n = 25; TRPM3 + Copine-6, n = 28. Data are shown as mean ± SEM. NM_001035242.1 /α2: -80 mV, ** P = 0.002; +80 mV, *** P < 0.0001. NM_001035240.2 /α3: −80 mV, ** P = 0.003; +80 mV, ** P = 0.0003 (two-tailed independent Student’s t-test). .
S1413 Sp141 Medchemexpress, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/HY-110184/SP-141/pm37963021-238-51-53
Average 94 stars, based on 1 article reviews
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bip inducer x  (MedChemExpress)
93
MedChemExpress bip inducer x
( A ) Time course (left) and I-V relationship (right) of a whole-cell currents induced by CIM0216 (5 μM) in CHO cells expressing TRPM3 (top) or TRPM3 + Copine-6 (bottom). ( B ) Voltage dependence of CIM0216-induced currents in TRPM3-expressing CHO cells with (red) and without (black) Copine-6 co-expression. Currents were induced by voltage ramps from −150 to +150 mV in 900 ms. Current-voltage relationships normalized to I TRPM3 at +150 mV are shown. TRPM3, n = 12; TRPM3 + Copine-6, n = 19. ( C ) Comparison of 5 μM CIM0216-induced current densities in CHO cells expressing TRPM3 ( NM_177341.4 /γ3), either alone or together with Copine-6 at −80 or +80 mV. TRPM3, n = 12; TRPM3 + Copine-6, n = 19; Data are shown as mean ± SEM; −80 mV, *** P < 0.001; +80 mV, ** P = 0.003 (two-tailed independent t test). ( D ) Current traces obtained with a voltage step protocol (inset on the bottom-left) in CHO cells transfected with TRPM3 alone (black) or co-transfected with Copine-6 (red) after stimulation by 5 μM CIM0216. ( E ) Voltage dependence of CIM0216-induced currents in TRPM3-expressing CHO cells with (red) and without (black) Copine-6 co-expression. Current-voltage relationships normalized to I TRPM3 at +200 mV are shown. TRPM3, n = 13; TRPM3 + Copine-6, n = 19; Data are shown as mean ± SEM; * P = 0.0188 (two-way ANOVA followed by Bonferroni multiple comparisons test). ( F ) Concentration-response curves for <t>Pregnenolone</t> <t>monosulfate</t> sodium (PregS) in CHO cells transfected with TRPM3 alone (black) or co-transfected with Copine-6 (red) at −80 mV (left) and +80 mV (right); recordings as these shown in ( A ). The EC 50 values are shown next to the curves. Current densities (pA/pF) represent the peak values of the PregS-induced currents. The number of recordings, n (from low to high concentration of PregS, respectively): 22, 18, 17, 20 22, and 20 for cells expressing TRPM3 alone (black); 20, 20, 20, 20, 19 and 18 for cells expressing TRPM3 and Copine-6 (red). Data are shown as mean ± SEM. All curves were fitted with Four-Parameter Logistic function. ( G ) Similar to ( F ), but CIM0216 concentration-response curves are plotted. The number of recordings, n (from low to high concentration of CIM0216, respectively): 17, 16, 16, 17 15, and 12 for cells expressing TRPM3 alone (black); 17, 17, 17, 14, 13 and 13 for cells expressing TRPM3 and Copine-6 (red). Data are shown as mean ± SEM. ( H ) Comparison of 5 μM CIM0216-induced current densities in CHO cells expressing TRPM3 splice variants ( NM_001035242.1 /α2 or NM_001035240.2 /α3), either alone or together with Copine-6 at −80 or +80 mV. NM_001035242.1 /α2: TRPM3, n = 16; TRPM3 + Copine-6, n = 22; NM_001035240.2 /α3: TRPM3, n = 25; TRPM3 + Copine-6, n = 28. Data are shown as mean ± SEM. NM_001035242.1 /α2: -80 mV, ** P = 0.002; +80 mV, *** P < 0.0001. NM_001035240.2 /α3: −80 mV, ** P = 0.003; +80 mV, ** P = 0.0003 (two-tailed independent Student’s t-test). .
Bip Inducer X, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/HY-110184/BiP+inducer+X/pm38252317-265-8-12
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brain concentrations  (MedChemExpress)
94
MedChemExpress brain concentrations
( A ) Time course (left) and I-V relationship (right) of a whole-cell currents induced by CIM0216 (5 μM) in CHO cells expressing TRPM3 (top) or TRPM3 + Copine-6 (bottom). ( B ) Voltage dependence of CIM0216-induced currents in TRPM3-expressing CHO cells with (red) and without (black) Copine-6 co-expression. Currents were induced by voltage ramps from −150 to +150 mV in 900 ms. Current-voltage relationships normalized to I TRPM3 at +150 mV are shown. TRPM3, n = 12; TRPM3 + Copine-6, n = 19. ( C ) Comparison of 5 μM CIM0216-induced current densities in CHO cells expressing TRPM3 ( NM_177341.4 /γ3), either alone or together with Copine-6 at −80 or +80 mV. TRPM3, n = 12; TRPM3 + Copine-6, n = 19; Data are shown as mean ± SEM; −80 mV, *** P < 0.001; +80 mV, ** P = 0.003 (two-tailed independent t test). ( D ) Current traces obtained with a voltage step protocol (inset on the bottom-left) in CHO cells transfected with TRPM3 alone (black) or co-transfected with Copine-6 (red) after stimulation by 5 μM CIM0216. ( E ) Voltage dependence of CIM0216-induced currents in TRPM3-expressing CHO cells with (red) and without (black) Copine-6 co-expression. Current-voltage relationships normalized to I TRPM3 at +200 mV are shown. TRPM3, n = 13; TRPM3 + Copine-6, n = 19; Data are shown as mean ± SEM; * P = 0.0188 (two-way ANOVA followed by Bonferroni multiple comparisons test). ( F ) Concentration-response curves for <t>Pregnenolone</t> <t>monosulfate</t> sodium (PregS) in CHO cells transfected with TRPM3 alone (black) or co-transfected with Copine-6 (red) at −80 mV (left) and +80 mV (right); recordings as these shown in ( A ). The EC 50 values are shown next to the curves. Current densities (pA/pF) represent the peak values of the PregS-induced currents. The number of recordings, n (from low to high concentration of PregS, respectively): 22, 18, 17, 20 22, and 20 for cells expressing TRPM3 alone (black); 20, 20, 20, 20, 19 and 18 for cells expressing TRPM3 and Copine-6 (red). Data are shown as mean ± SEM. All curves were fitted with Four-Parameter Logistic function. ( G ) Similar to ( F ), but CIM0216 concentration-response curves are plotted. The number of recordings, n (from low to high concentration of CIM0216, respectively): 17, 16, 16, 17 15, and 12 for cells expressing TRPM3 alone (black); 17, 17, 17, 14, 13 and 13 for cells expressing TRPM3 and Copine-6 (red). Data are shown as mean ± SEM. ( H ) Comparison of 5 μM CIM0216-induced current densities in CHO cells expressing TRPM3 splice variants ( NM_001035242.1 /α2 or NM_001035240.2 /α3), either alone or together with Copine-6 at −80 or +80 mV. NM_001035242.1 /α2: TRPM3, n = 16; TRPM3 + Copine-6, n = 22; NM_001035240.2 /α3: TRPM3, n = 25; TRPM3 + Copine-6, n = 28. Data are shown as mean ± SEM. NM_001035242.1 /α2: -80 mV, ** P = 0.002; +80 mV, *** P < 0.0001. NM_001035240.2 /α3: −80 mV, ** P = 0.003; +80 mV, ** P = 0.0003 (two-tailed independent Student’s t-test). .
Brain Concentrations, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


( A ) Time course (left) and I-V relationship (right) of a whole-cell currents induced by CIM0216 (5 μM) in CHO cells expressing TRPM3 (top) or TRPM3 + Copine-6 (bottom). ( B ) Voltage dependence of CIM0216-induced currents in TRPM3-expressing CHO cells with (red) and without (black) Copine-6 co-expression. Currents were induced by voltage ramps from −150 to +150 mV in 900 ms. Current-voltage relationships normalized to I TRPM3 at +150 mV are shown. TRPM3, n = 12; TRPM3 + Copine-6, n = 19. ( C ) Comparison of 5 μM CIM0216-induced current densities in CHO cells expressing TRPM3 ( NM_177341.4 /γ3), either alone or together with Copine-6 at −80 or +80 mV. TRPM3, n = 12; TRPM3 + Copine-6, n = 19; Data are shown as mean ± SEM; −80 mV, *** P < 0.001; +80 mV, ** P = 0.003 (two-tailed independent t test). ( D ) Current traces obtained with a voltage step protocol (inset on the bottom-left) in CHO cells transfected with TRPM3 alone (black) or co-transfected with Copine-6 (red) after stimulation by 5 μM CIM0216. ( E ) Voltage dependence of CIM0216-induced currents in TRPM3-expressing CHO cells with (red) and without (black) Copine-6 co-expression. Current-voltage relationships normalized to I TRPM3 at +200 mV are shown. TRPM3, n = 13; TRPM3 + Copine-6, n = 19; Data are shown as mean ± SEM; * P = 0.0188 (two-way ANOVA followed by Bonferroni multiple comparisons test). ( F ) Concentration-response curves for Pregnenolone monosulfate sodium (PregS) in CHO cells transfected with TRPM3 alone (black) or co-transfected with Copine-6 (red) at −80 mV (left) and +80 mV (right); recordings as these shown in ( A ). The EC 50 values are shown next to the curves. Current densities (pA/pF) represent the peak values of the PregS-induced currents. The number of recordings, n (from low to high concentration of PregS, respectively): 22, 18, 17, 20 22, and 20 for cells expressing TRPM3 alone (black); 20, 20, 20, 20, 19 and 18 for cells expressing TRPM3 and Copine-6 (red). Data are shown as mean ± SEM. All curves were fitted with Four-Parameter Logistic function. ( G ) Similar to ( F ), but CIM0216 concentration-response curves are plotted. The number of recordings, n (from low to high concentration of CIM0216, respectively): 17, 16, 16, 17 15, and 12 for cells expressing TRPM3 alone (black); 17, 17, 17, 14, 13 and 13 for cells expressing TRPM3 and Copine-6 (red). Data are shown as mean ± SEM. ( H ) Comparison of 5 μM CIM0216-induced current densities in CHO cells expressing TRPM3 splice variants ( NM_001035242.1 /α2 or NM_001035240.2 /α3), either alone or together with Copine-6 at −80 or +80 mV. NM_001035242.1 /α2: TRPM3, n = 16; TRPM3 + Copine-6, n = 22; NM_001035240.2 /α3: TRPM3, n = 25; TRPM3 + Copine-6, n = 28. Data are shown as mean ± SEM. NM_001035242.1 /α2: -80 mV, ** P = 0.002; +80 mV, *** P < 0.0001. NM_001035240.2 /α3: −80 mV, ** P = 0.003; +80 mV, ** P = 0.0003 (two-tailed independent Student’s t-test). .

Journal: The EMBO Journal

Article Title: Copine-6 is a TRPM3 escort protein controlling the sensitivity of sensory neurons to noxious heat

doi: 10.1038/s44318-025-00487-0

Figure Lengend Snippet: ( A ) Time course (left) and I-V relationship (right) of a whole-cell currents induced by CIM0216 (5 μM) in CHO cells expressing TRPM3 (top) or TRPM3 + Copine-6 (bottom). ( B ) Voltage dependence of CIM0216-induced currents in TRPM3-expressing CHO cells with (red) and without (black) Copine-6 co-expression. Currents were induced by voltage ramps from −150 to +150 mV in 900 ms. Current-voltage relationships normalized to I TRPM3 at +150 mV are shown. TRPM3, n = 12; TRPM3 + Copine-6, n = 19. ( C ) Comparison of 5 μM CIM0216-induced current densities in CHO cells expressing TRPM3 ( NM_177341.4 /γ3), either alone or together with Copine-6 at −80 or +80 mV. TRPM3, n = 12; TRPM3 + Copine-6, n = 19; Data are shown as mean ± SEM; −80 mV, *** P < 0.001; +80 mV, ** P = 0.003 (two-tailed independent t test). ( D ) Current traces obtained with a voltage step protocol (inset on the bottom-left) in CHO cells transfected with TRPM3 alone (black) or co-transfected with Copine-6 (red) after stimulation by 5 μM CIM0216. ( E ) Voltage dependence of CIM0216-induced currents in TRPM3-expressing CHO cells with (red) and without (black) Copine-6 co-expression. Current-voltage relationships normalized to I TRPM3 at +200 mV are shown. TRPM3, n = 13; TRPM3 + Copine-6, n = 19; Data are shown as mean ± SEM; * P = 0.0188 (two-way ANOVA followed by Bonferroni multiple comparisons test). ( F ) Concentration-response curves for Pregnenolone monosulfate sodium (PregS) in CHO cells transfected with TRPM3 alone (black) or co-transfected with Copine-6 (red) at −80 mV (left) and +80 mV (right); recordings as these shown in ( A ). The EC 50 values are shown next to the curves. Current densities (pA/pF) represent the peak values of the PregS-induced currents. The number of recordings, n (from low to high concentration of PregS, respectively): 22, 18, 17, 20 22, and 20 for cells expressing TRPM3 alone (black); 20, 20, 20, 20, 19 and 18 for cells expressing TRPM3 and Copine-6 (red). Data are shown as mean ± SEM. All curves were fitted with Four-Parameter Logistic function. ( G ) Similar to ( F ), but CIM0216 concentration-response curves are plotted. The number of recordings, n (from low to high concentration of CIM0216, respectively): 17, 16, 16, 17 15, and 12 for cells expressing TRPM3 alone (black); 17, 17, 17, 14, 13 and 13 for cells expressing TRPM3 and Copine-6 (red). Data are shown as mean ± SEM. ( H ) Comparison of 5 μM CIM0216-induced current densities in CHO cells expressing TRPM3 splice variants ( NM_001035242.1 /α2 or NM_001035240.2 /α3), either alone or together with Copine-6 at −80 or +80 mV. NM_001035242.1 /α2: TRPM3, n = 16; TRPM3 + Copine-6, n = 22; NM_001035240.2 /α3: TRPM3, n = 25; TRPM3 + Copine-6, n = 28. Data are shown as mean ± SEM. NM_001035242.1 /α2: -80 mV, ** P = 0.002; +80 mV, *** P < 0.0001. NM_001035240.2 /α3: −80 mV, ** P = 0.003; +80 mV, ** P = 0.0003 (two-tailed independent Student’s t-test). .

Article Snippet: Pregnenolone monosulfate sodium , MedChemExpress , Cat# HY-110189.

Techniques: Expressing, Comparison, Two Tailed Test, Transfection, Concentration Assay