Journal: Cell Death and Differentiation

Article Title: p53 transcriptionally activates DCP1B to suppress tumor progression and enhance tumor sensitivity to PI3K blockade in non-small cell lung cancer

doi: 10.1038/s41418-025-01501-y

Figure Lengend Snippet: A , B WB assays assessing the expression levels of downstream proteins in A549 and H460 cells stably expressing shNC and shMAPK4. C , D WB assays assessing the expression levels of downstream proteins in A549 and H460 cells stably expressing PCDH and PCDH-MAPK4. E , F WB assays assessing the expression levels of downstream proteins in A549 and H460 cells stably expressing PCDH and PCDH-MAPK4 treated with or without PI3K inhibitor Alpelisib. G – J RT-Qpcr and WB assays validating the expression levels of MAPK4 in A549 and H460 treated with gradually increasing drug concentrations of p53-inducing agent APG-115 for 24 h. Data in G and I are represented as mean ± SD, n = 3.

Article Snippet: Nutlin-3, 5-fluorouracil (5-Fu), Cisplatin, Etoposide, APG-115, Alpelisib, Actinomycin D (Act.D), and Dimethyl sulfoxide (DMSO) were purchased from MedChemExpress (MCE, Shanghai, China).

Techniques: Expressing, Stable Transfection, Quantitative RT-PCR

Journal: Cell Death and Differentiation

Article Title: p53 transcriptionally activates DCP1B to suppress tumor progression and enhance tumor sensitivity to PI3K blockade in non-small cell lung cancer

doi: 10.1038/s41418-025-01501-y

Figure Lengend Snippet: A – H Drug-sensitivity and the combination index (CI) of 2 drugs assays showing the synergistic inhibition effect of p53 agonist and PI3K inhibitor in A549 and H460 cells in vitro. Data in A , C , E and G are represented as mean ± SD, n = 3. I – M A549 cells were inoculated into BALB/c female nude mice. After 14 days of injection, mice were randomly divided into four groups including the control group, APG-115 group, Alpelisib group, and the co-drug group. After 3 weeks, mice were sacrificed and xenograft tumors were removed. Representative mice weight, tumor volume, tumor weight, and tumor images are shown. N The proposed working model. Data in K and L are represented as mean ± SD, n = 7.

Article Snippet: Nutlin-3, 5-fluorouracil (5-Fu), Cisplatin, Etoposide, APG-115, Alpelisib, Actinomycin D (Act.D), and Dimethyl sulfoxide (DMSO) were purchased from MedChemExpress (MCE, Shanghai, China).

Techniques: Inhibition, In Vitro, Injection, Control

Journal: eLife

Article Title: Telomere dysfunction cooperates with epigenetic alterations to impair murine embryonic stem cell fate commitment

doi: 10.7554/elife.47333

Figure Lengend Snippet: Figure 3. Inhibition of Kdm6a/b histone demethyltransferase activity partially rescues cell fate commitment. (A, C) Western blot analysis of H3K27me3 or Nanog protein levels in WT and Tert-/- mESCs, in the presence of DMSO only, GSKJ4 or GSKJ5. For clarity, no image cropping was performed between lanes and the blot includes an independent replicate of the A395/A395N treatments shown in Figure 2. Hsp90ab1 and H3 are used as loading control for Nanog and H3K27me3 respectively. Representative blots from n = 3 biological replicates. Quantification of western blots is shown in Figure 3—figure supplement 1D,G. (B) FACS analysis of Pou5f1-GFP reporter expression in mESCs. Data are represented as mean ± SD (n = 3 biological replicates each with three technical replicates). Statistical analysis was performed using two-way ANOVA, * (p<0.0332), ** (p<0.0021), *** (p<0.0002), **** (p<0.0001). (D) Heatmap representation of relative fold change in gene expression of 77 lineage-specific markers (endoderm, mesoderm and ectoderm) and five pluripotency genes from the Qiagen Mouse Cell Lineage Identification qPCR Array (n = 3 biological replicates). Gene expression analysis was performed on 6 DA + 6 DL mESCs treated with the Kdm6a/b inhibitor (GSKJ4), the inactive molecule (GSKJ5) or DMSO control. Relative fold change in gene expression was calculated using Tert-/- DMSO as control (fold change = 1) and normalized to five housekeeping genes: Actb, B2m, Gapdh, Gusb and Hsp90ab1. The color scale represents the row centered fold change values (blue = lower, white = intermediate, red = higher). Euclidean distance clustering and complete linkage was applied to visualize similarity between samples. The online version of this article includes the following source data and figure supplement(s) for figure 3:

Article Snippet: Compounds were provided by the Structural Genomics Consortium or were purchased, for example: A395, A395N (Sigma, cat. # SML1879-25MG, SML1923-5MG) and GSKJ4, GSK343 (Medchem Express, NY, USA, distributed in Canada by Cedarlane, Burlington, ON, Canada, cat. # HY-15648B, HY-13500).

Techniques: Inhibition, Activity Assay, Western Blot, Control, Expressing, Gene Expression