Journal: BMC Cancer

Article Title: Cervical squamous carcinoma cells are resistant to the combined action of tumor necrosis factor-α and histamine whereas normal keratinocytes undergo cytolysis

doi: 10.1186/1471-2407-8-46

Figure Lengend Snippet: The effect of TNF-α on the DNA content of SiHa cells. The cells were grown in incomplete and complete MEM and treated with indicated concentrations of TNF-α. The data are expressed as the percentage of the fluorescence in the control wells. Each bar represents the mean of individual quadruplicate cultures ± SE (n = 4). "*" denotes p < 0.05 when compared with the control values.

Article Snippet: Recombinant human TNF-α, goat anti-human TNFRI/TNFRSF1A polyclonal antibody, and goat anti-human TNFRII/TNFRSF1B (TNF-BPII) polyclonal antibody were from R&D Systems Europe Ltd (Oxon, UK) and rabbit polyclonal antibody against human TNF-α from HyCult biotechnology b.v. (Uden, The Netherlands).

Techniques: Fluorescence

Journal: BMC Cancer

Article Title: Cervical squamous carcinoma cells are resistant to the combined action of tumor necrosis factor-α and histamine whereas normal keratinocytes undergo cytolysis

doi: 10.1186/1471-2407-8-46

Figure Lengend Snippet: The effect of histamine (1 mM) and/or TNF-α (10 and 50 ng/ml) on the DNA content of normal keratinocytes. The cells were cultivated in incomplete or complete Keratinocyte-SFM medium and treated with indicated concentrations of histamine and/or TNF-α. The results are expressed as the percentage of the fluorescence in the control wells. Each bar represents the mean of four individual quadruplicate cultures ± SE. "*" denotes p < 0.05 when compared with the control values.

Article Snippet: Recombinant human TNF-α, goat anti-human TNFRI/TNFRSF1A polyclonal antibody, and goat anti-human TNFRII/TNFRSF1B (TNF-BPII) polyclonal antibody were from R&D Systems Europe Ltd (Oxon, UK) and rabbit polyclonal antibody against human TNF-α from HyCult biotechnology b.v. (Uden, The Netherlands).

Techniques: Fluorescence

Journal: BMC Cancer

Article Title: Cervical squamous carcinoma cells are resistant to the combined action of tumor necrosis factor-α and histamine whereas normal keratinocytes undergo cytolysis

doi: 10.1186/1471-2407-8-46

Figure Lengend Snippet: The effect of histamine and/or TNF-α on the DNA content of SiHa and ME-180 cells. SiHa cells were cultured with 0.1 mM histamine and/or TNF-α (a) or with 1 mM histamine and/or TNF-α (b) using incomplete or complete MEM. ME-180 cells were cultured with 1 mM histamine and/or TNF-α (c) using incomplete or complete McCoy's 5a medium. The results are expressed as the percentage of the fluorescence in the control wells. Each bar represents the mean of four individual quadruplicate cultures ± SE. "*" denotes p < 0.05 when compared with the control values.

Article Snippet: Recombinant human TNF-α, goat anti-human TNFRI/TNFRSF1A polyclonal antibody, and goat anti-human TNFRII/TNFRSF1B (TNF-BPII) polyclonal antibody were from R&D Systems Europe Ltd (Oxon, UK) and rabbit polyclonal antibody against human TNF-α from HyCult biotechnology b.v. (Uden, The Netherlands).

Techniques: Cell Culture, Fluorescence

Journal: BMC Cancer

Article Title: Cervical squamous carcinoma cells are resistant to the combined action of tumor necrosis factor-α and histamine whereas normal keratinocytes undergo cytolysis

doi: 10.1186/1471-2407-8-46

Figure Lengend Snippet: The cytotoxic effect of histamine and/or TNF-α on keratinocytes (a), SiHa cells (b) and ME-180 cells (c). The cells were cultivated in incomplete or complete medium and treated with indicated concentrations of histamine and/or TNF-α. The results are expressed as the percentage of the absorbance in the control wells. Each bar represents the mean of four individual cultures ± SE. "*" denotes p < 0.05 when compared with the control values.

Article Snippet: Recombinant human TNF-α, goat anti-human TNFRI/TNFRSF1A polyclonal antibody, and goat anti-human TNFRII/TNFRSF1B (TNF-BPII) polyclonal antibody were from R&D Systems Europe Ltd (Oxon, UK) and rabbit polyclonal antibody against human TNF-α from HyCult biotechnology b.v. (Uden, The Netherlands).

Techniques:

Journal: BMC Cancer

Article Title: Cervical squamous carcinoma cells are resistant to the combined action of tumor necrosis factor-α and histamine whereas normal keratinocytes undergo cytolysis

doi: 10.1186/1471-2407-8-46

Figure Lengend Snippet: The effect of histamine and/or TNF-α on SiHa cells with and without inhibition of protein synthesis. The cells were pretreated with emetine and then cultivated and treated with indicated concentrations of histamine and/or TNF-α. The results are expressed as the percentage of the absorbance in the control wells. Each bar represents the mean of three individual cultures ± SD. "*" denotes p < 0.05 when compared with the control values.

Article Snippet: Recombinant human TNF-α, goat anti-human TNFRI/TNFRSF1A polyclonal antibody, and goat anti-human TNFRII/TNFRSF1B (TNF-BPII) polyclonal antibody were from R&D Systems Europe Ltd (Oxon, UK) and rabbit polyclonal antibody against human TNF-α from HyCult biotechnology b.v. (Uden, The Netherlands).

Techniques: Inhibition

Journal: BMC Cancer

Article Title: Cervical squamous carcinoma cells are resistant to the combined action of tumor necrosis factor-α and histamine whereas normal keratinocytes undergo cytolysis

doi: 10.1186/1471-2407-8-46

Figure Lengend Snippet: The effect of histamine and/or TNF-α on the growth of keratinocyte epithelium in a representative 3-day culture by using 10% fetal calf serum and DMEM as the culture medium. The following agents were added to the wells: well 1, diluent control; well 2, 0.1 mM histamine; well 3, 10 ng mL -1 TNF-α; well 4, 50 ng mL -1 TNF-α; well 5, 0.1 mM histamine + 10 ng mL -1 TNF-α; well 6, 0.1 mM histamine + 50 ng mL -1 TNF-α. Histamine (0.1 mM) or TNF-α (10 or 50 ng/ml) alone inhibits the growth of epithelium and the growth-inhibitory effect is increased when both agents are added to the same well.

Article Snippet: Recombinant human TNF-α, goat anti-human TNFRI/TNFRSF1A polyclonal antibody, and goat anti-human TNFRII/TNFRSF1B (TNF-BPII) polyclonal antibody were from R&D Systems Europe Ltd (Oxon, UK) and rabbit polyclonal antibody against human TNF-α from HyCult biotechnology b.v. (Uden, The Netherlands).

Techniques:

Journal: BMC Cancer

Article Title: Cervical squamous carcinoma cells are resistant to the combined action of tumor necrosis factor-α and histamine whereas normal keratinocytes undergo cytolysis

doi: 10.1186/1471-2407-8-46

Figure Lengend Snippet: The effect of histamine and/or TNF-α on SiHa cell migration in the transwell assay. TNF-α significantly increased the cell migration but addition of histamine could not produce any additional effect over that by TNF-α alone. The results are expressed as the percentage of migrated cells compared to those in control wells. (n = 3; mean ± SD). The control is indicated as 100%. "*" denotes p < 0.05 when compared with control values.

Article Snippet: Recombinant human TNF-α, goat anti-human TNFRI/TNFRSF1A polyclonal antibody, and goat anti-human TNFRII/TNFRSF1B (TNF-BPII) polyclonal antibody were from R&D Systems Europe Ltd (Oxon, UK) and rabbit polyclonal antibody against human TNF-α from HyCult biotechnology b.v. (Uden, The Netherlands).

Techniques: Migration, Transwell Assay

Journal: BMC Cancer

Article Title: Cervical squamous carcinoma cells are resistant to the combined action of tumor necrosis factor-α and histamine whereas normal keratinocytes undergo cytolysis

doi: 10.1186/1471-2407-8-46

Figure Lengend Snippet: TNF-α positive cells in the cervix carcinoma – immunohistochemical staining of SCC specimens. Note: strong immunopositivity of the epithelial cells of control sample (a); TNF-α positive tumor cells and peritumoral stroma in cervix carcinoma (b).

Article Snippet: Recombinant human TNF-α, goat anti-human TNFRI/TNFRSF1A polyclonal antibody, and goat anti-human TNFRII/TNFRSF1B (TNF-BPII) polyclonal antibody were from R&D Systems Europe Ltd (Oxon, UK) and rabbit polyclonal antibody against human TNF-α from HyCult biotechnology b.v. (Uden, The Netherlands).

Techniques: Immunohistochemical staining, Staining

Journal: BMC Cancer

Article Title: Cervical squamous carcinoma cells are resistant to the combined action of tumor necrosis factor-α and histamine whereas normal keratinocytes undergo cytolysis

doi: 10.1186/1471-2407-8-46

Figure Lengend Snippet: The presence of TNF-α positive cells in the cervix carcinoma and control samples. Score of TNF-α

Article Snippet: Recombinant human TNF-α, goat anti-human TNFRI/TNFRSF1A polyclonal antibody, and goat anti-human TNFRII/TNFRSF1B (TNF-BPII) polyclonal antibody were from R&D Systems Europe Ltd (Oxon, UK) and rabbit polyclonal antibody against human TNF-α from HyCult biotechnology b.v. (Uden, The Netherlands).

Techniques:

Journal: Prostaglandins, leukotrienes, and essential fatty acids

Article Title: Fatty acid metabolism is involved in both retinal physiology and the pathology of retinal vascular diseases.

doi: 10.1016/j.plefa.2022.102473

Figure Lengend Snippet: Fig. 1. Representative immunofluorescent labeling of healthy human retina with several anti-FABPs. Paraffine-fixed healthy human retina was immunolabeled with anti-FABP3, FABP4, FABP5, FABP7 FABP8 or FABP12 as described in the methods section, and representative merged images of the immunolabeling with DAPI are shown. Scale bar; 25 µm. Experiments were repeated twice at a minimum to confirm that the staining is correct, and among these, representative images were used. FABP; fatty acid binding protein, NFL; nerve fiber layer, GCL; ganglion cell layer, IPL; inner plexiform layer, INL; inner nuclear layer, OPL; outer nuclear layer, ONL; outer nuclear layer, OS; outer segment, RPE; retinal pigment epithelium.

Article Snippet: After the deparaffinization, the sections were each blocked by treatment with 3% bovine serum albumin (BSA) for 1 hour, and successively incubated with the 1st antibody including anti-FABP3 (#10,676-AP, Proteintech, Rosemont, IL, USA), FABP4 (#13,979, Abcam, Eugene, OR, USA), FABP5 (#HP9030–100UG, Wayne, PA, USA), FABP7 (#51,010–1-AP, Proteintech, Rosemont, IL, USA), FABP8 (#12,717–1-AP, Proteintech, Rosemont, IL, USA) or FABP12 (#155,089, Abcam, Eugene, OR, USA) for 3 hrs, and the 2nd antibody; a goat anti-rabbit IgG (488 nm, 1:1000 dilutions) and DAPI (1:1000 dilutions) for 3 hrs.

Techniques: Labeling, Immunolabeling, Staining, Binding Assay

Journal: Prostaglandins, leukotrienes, and essential fatty acids

Article Title: Fatty acid metabolism is involved in both retinal physiology and the pathology of retinal vascular diseases.

doi: 10.1016/j.plefa.2022.102473

Figure Lengend Snippet: Fig. 2. Representative immunofluorescent labeling of wild type (WT), SDT and P23H rats and WT mouse retinas with several anti-FAPB4 antibodies. Paraffin-fixed Rat (WT, SDT or P23H) rat or WT mouse retina was immunolabeled with anti-FABP3, FABP4, FABP5, FABP7, FABP8 or FABP12 as described in the methods section. Representative immunolabeling images (left) and merged images with DAPI (right) of samples stained with FABP4 and FABP12 are shown, since no staining was observed by other FABP antibodies as shown in Table 1. Scale bar; 25 µm. Experiments were repeated twice at a minimum to confirm that the staining is correct, and among these, the representative images were used. FABP; fatty acid binding protein, NFL; nerve fiber layer, GCL; ganglion cell layer, IPL; inner plexiform layer, INL; inner nuclear layer, OPL; outer nuclear layer, ONL; outer nuclear layer, OS; outer segment, RPE; retinal pigment epithelium.

Article Snippet: After the deparaffinization, the sections were each blocked by treatment with 3% bovine serum albumin (BSA) for 1 hour, and successively incubated with the 1st antibody including anti-FABP3 (#10,676-AP, Proteintech, Rosemont, IL, USA), FABP4 (#13,979, Abcam, Eugene, OR, USA), FABP5 (#HP9030–100UG, Wayne, PA, USA), FABP7 (#51,010–1-AP, Proteintech, Rosemont, IL, USA), FABP8 (#12,717–1-AP, Proteintech, Rosemont, IL, USA) or FABP12 (#155,089, Abcam, Eugene, OR, USA) for 3 hrs, and the 2nd antibody; a goat anti-rabbit IgG (488 nm, 1:1000 dilutions) and DAPI (1:1000 dilutions) for 3 hrs.

Techniques: Labeling, Immunolabeling, Staining, Binding Assay