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Image Search Results
Journal: JCI Insight
Article Title: Pravastatin therapy during preeclampsia prevents long-term adverse health effects in mice
doi: 10.1172/jci.insight.120147
Figure Lengend Snippet: Protective effects of pravastatin. Glomerular lesions were evaluated by immunohistochemistry. The glomerular lesions observed during and after preeclampsia (PE), characterized by increased tissue factor, fibrin, and complement C3b/iC3b/C3d expression/deposition are focal, and within the affected glomeruli, the pathological markers are global. Collagen deposition and α-smooth muscle actin (ASMA) expression in the glomeruli from mothers after PE are also focal and global. Representative images of the affected glomeruli are shown. (A) Immunohistochemical (IHC) detection of tissue factor, fibrin, and complement activation product C3b/iC3b/C3d in glomeruli from maternal kidneys harvested before, during, and after pregnancy in control mice, PE mice, and PE+PRAV mice. Scale bar: 20 μm. Five to six images per experimental group (n = 6–7 mice/group) and ten glomeruli per slide were analyzed. (B) IHC detection of collagen I (red fluorescence) and ASMA (green fluorescence) staining in maternal kidneys after normal pregnancies (control) and preeclamptic pregnancies (untreated [PE] and treated with pravastatin [PE+PRAV]). Scale bar: 50 μm. Five to six images per experimental group (n = 6–7 mice/group) and ten glomeruli per slide were analyzed. (C) Quantification of IHC staining of tissue factor, fibrin, and C3b/iC3b/C3d and collagen I and ASMA using ImageJ. Five to six images per experimental group (n = 6–7 mice/group) and ten glomeruli per slide were analyzed. Red squares represent the mean. Comparisons between groups were performed by 1-way ANOVA with Bonferroni’s post hoc test.*P < 0.01, statistically different from control at same time.
Article Snippet: Kidney cryostatic sections (5 μm) were then incubated with primary antibodies directed to mouse fibrinogen/fibrin (Abcam, ab34269), tissue factor (Abcam, ab151748), and
Techniques: Immunohistochemistry, Expressing, Immunohistochemical staining, Activation Assay, Fluorescence, Staining