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Image Search Results
Journal: Cancer Science
Article Title: Systemic NK4 gene therapy inhibits tumor growth and metastasis of melanoma and lung carcinoma in syngeneic mouse tumor models
doi: 10.1111/j.1349-7006.2009.01184.x
Figure Lengend Snippet: Inhibitory effects of NK4 on invasion of B16F10 and Lewis lung carcinoma (LLC) cells. Typical appearances of (a) B16F10 and (c) LLC cells that invaded through the Matrigel and a membrane. Change in the number of invasive cells: (b) B16F10 or (d) LLC cells were cultured for 36 h. Each value represents the means ± SEM (n = 3 in each group). HGF, hepatocyte growth factor.
Article Snippet: Recombinant HGF and NK4 were purified from the conditioned medium of CHO cells transfected with cDNA for
Techniques: Membrane, Cell Culture
Journal: Cancer Science
Article Title: Systemic NK4 gene therapy inhibits tumor growth and metastasis of melanoma and lung carcinoma in syngeneic mouse tumor models
doi: 10.1111/j.1349-7006.2009.01184.x
Figure Lengend Snippet: Adenovirus‐mediated NK4 gene expression in vitro. (a) Expression of NK4 protein. Cell lysates from HEK293 cells were analyzed by immunoprecipitation and western blotting using antihuman hepatocyte growth factor (HGF) antibody to detect NK4 (67 kDa). The α‐chain of HGF (69 kDa), also detected by the same antibody, was used as a control. (b) Inhibitory effect of adenovirally expressed NK4 on invasion of cancer cells. SUIT2 cells were infected with Ad.NK4 or Ad.LacZ and subjected to Matrigel invasion assay either with or without HGF (110 pM). (c) Inefficient adenovirus‐mediated NK4 induction in B16F10 and Lewis lung carcinoma (LLC) cells. SUIT2, B16F10, and LLC cells were infected with either Ad.NK4 or Ad.LacZ, and culture media were harvested for measurement of NK4. Each value represents the means ± SEM (n = 3 in each group).
Article Snippet: Recombinant HGF and NK4 were purified from the conditioned medium of CHO cells transfected with cDNA for
Techniques: Expressing, In Vitro, Immunoprecipitation, Western Blot, Infection, Invasion Assay
Journal: Cancer Science
Article Title: Systemic NK4 gene therapy inhibits tumor growth and metastasis of melanoma and lung carcinoma in syngeneic mouse tumor models
doi: 10.1111/j.1349-7006.2009.01184.x
Figure Lengend Snippet: Inhibitory effects of intravenous Ad.NK4 administration on primary tumor growth. Growth curves of subcutaneous tumor growth of (a) Lewis lung carcinoma (LLC) or (b) B16F10, and (c) NK4 and (d) hepatocyte growth factor (HGF) levels in tumor tissues on day 19. LLC or B16F10 cells were subcutaneously implanted in the right flank of mice on day 0. Vehicle, Ad.NK4, or Ad.LacZ was administered intravenously once on day 1. Each value represents the mean ± SEM (n = 8 in each group).
Article Snippet: Recombinant HGF and NK4 were purified from the conditioned medium of CHO cells transfected with cDNA for
Techniques: