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  • 94
    Millipore hdac3
    Intrahippocampal RGFP136 infusions increases histone acetylation. Images on left are 4×, and 20× magnifications of the regions boxed in white are on the right. Histograms depict quantification of optical density as a percentage of vehicle. A , <t>HDAC3</t> immunoreactivity is unaltered in area of infusion 2 h after RGFP136 treatment compared with vehicle. B , Representative images show HDAC2 immunoreactivity in dorsal hippocampus is also unchanged by drug treatment. C , However, HDAC4 nuclear immunoreactivity is decreased in the region of the RGFP136 infusion. * p
    Hdac3, supplied by Millipore, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hdac3/product/Millipore
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    94
    Millipore hdac inhibitors
    Histone deacetylases <t>(HDAC)</t> inhibitors regulate cytokine production. Expressions of IFN-γ and TNF-α were detected by quantitative real-time PCR and sandwich ELISA. (A,B) IFN-γ expression by γδ T cells stimulated with HDMAPP, treated with or without HDAC inhibitors sodium valproate <t>(VPA),</t> Trichostatin-A (TSA), and suberoylanilidehydroxamic acid (SAHA) at different concentrations at mRNA and protein levels, respectively. (C,D) Expression of TNF-α in the supernatants collected from HDMAPP stimulated γδ T cells in the presence or absence of HDAC inhibitors VPA, TSA, and SAHA at different concentrations at mRNA and protein levels, respectively. The expression of different m-RNA transcripts was normalized to 18S r-RNA. All the results indicated are mean ± SEM of three independent experiments, where * p
    Hdac Inhibitors, supplied by Millipore, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hdac inhibitors/product/Millipore
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
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    99
    BioVision hdac activity
    Histone deacetylases <t>(HDAC)</t> inhibitors regulate cytokine production. Expressions of IFN-γ and TNF-α were detected by quantitative real-time PCR and sandwich ELISA. (A,B) IFN-γ expression by γδ T cells stimulated with HDMAPP, treated with or without HDAC inhibitors sodium valproate <t>(VPA),</t> Trichostatin-A (TSA), and suberoylanilidehydroxamic acid (SAHA) at different concentrations at mRNA and protein levels, respectively. (C,D) Expression of TNF-α in the supernatants collected from HDMAPP stimulated γδ T cells in the presence or absence of HDAC inhibitors VPA, TSA, and SAHA at different concentrations at mRNA and protein levels, respectively. The expression of different m-RNA transcripts was normalized to 18S r-RNA. All the results indicated are mean ± SEM of three independent experiments, where * p
    Hdac Activity, supplied by BioVision, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hdac activity/product/BioVision
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    hdac activity - by Bioz Stars, 2021-12
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    86
    Selleck Chemicals hdac inhibitors
    Histone deacetylases <t>(HDAC)</t> inhibitors regulate cytokine production. Expressions of IFN-γ and TNF-α were detected by quantitative real-time PCR and sandwich ELISA. (A,B) IFN-γ expression by γδ T cells stimulated with HDMAPP, treated with or without HDAC inhibitors sodium valproate <t>(VPA),</t> Trichostatin-A (TSA), and suberoylanilidehydroxamic acid (SAHA) at different concentrations at mRNA and protein levels, respectively. (C,D) Expression of TNF-α in the supernatants collected from HDMAPP stimulated γδ T cells in the presence or absence of HDAC inhibitors VPA, TSA, and SAHA at different concentrations at mRNA and protein levels, respectively. The expression of different m-RNA transcripts was normalized to 18S r-RNA. All the results indicated are mean ± SEM of three independent experiments, where * p
    Hdac Inhibitors, supplied by Selleck Chemicals, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 86 stars, based on 1 article reviews
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    Image Search Results


    Intrahippocampal RGFP136 infusions increases histone acetylation. Images on left are 4×, and 20× magnifications of the regions boxed in white are on the right. Histograms depict quantification of optical density as a percentage of vehicle. A , HDAC3 immunoreactivity is unaltered in area of infusion 2 h after RGFP136 treatment compared with vehicle. B , Representative images show HDAC2 immunoreactivity in dorsal hippocampus is also unchanged by drug treatment. C , However, HDAC4 nuclear immunoreactivity is decreased in the region of the RGFP136 infusion. * p

    Journal: The Journal of neuroscience : the official journal of the Society for Neuroscience

    Article Title: HDAC3 Is a Critical Negative Regulator of Long-Term Memory Formation

    doi: 10.1523/JNEUROSCI.5052-10.2011

    Figure Lengend Snippet: Intrahippocampal RGFP136 infusions increases histone acetylation. Images on left are 4×, and 20× magnifications of the regions boxed in white are on the right. Histograms depict quantification of optical density as a percentage of vehicle. A , HDAC3 immunoreactivity is unaltered in area of infusion 2 h after RGFP136 treatment compared with vehicle. B , Representative images show HDAC2 immunoreactivity in dorsal hippocampus is also unchanged by drug treatment. C , However, HDAC4 nuclear immunoreactivity is decreased in the region of the RGFP136 infusion. * p

    Article Snippet: This distinct neural circuitry for the ORM and OLM tasks can reveal the specificity of our treatment. shows that, after subthreshold training (3 min), both HDAC3flox/flox ( n = 8) and HDAC3 +/+ ( n = 8) mice spent similar amounts of time with both the familiar and novel objects on test day ( t (14) = 0.40; p = 0.70).

    Techniques:

    Nr4a2 siRNA attenuates the long-term memory enhancement observed in HDAC3 flox/flox mice. A , At 48 h after infusions of Nr4a2 or RISC-free siRNA, HDAC3 flox/flox and HDAC3 +/+ mice received subthreshold training (3 min) in an environment with two identical objects and received a retention test 24 h later in which one object is moved to a new location. B , HDAC3 flox/flox mice infused with RISC free ( n = 10) exhibited significant memory for object location compared with HDAC3 +/+ mice ( †† p = 0.001), which was blocked by Nr4a2 siRNA treatment ( n = 9–10 per group; ** p

    Journal: The Journal of neuroscience : the official journal of the Society for Neuroscience

    Article Title: HDAC3 Is a Critical Negative Regulator of Long-Term Memory Formation

    doi: 10.1523/JNEUROSCI.5052-10.2011

    Figure Lengend Snippet: Nr4a2 siRNA attenuates the long-term memory enhancement observed in HDAC3 flox/flox mice. A , At 48 h after infusions of Nr4a2 or RISC-free siRNA, HDAC3 flox/flox and HDAC3 +/+ mice received subthreshold training (3 min) in an environment with two identical objects and received a retention test 24 h later in which one object is moved to a new location. B , HDAC3 flox/flox mice infused with RISC free ( n = 10) exhibited significant memory for object location compared with HDAC3 +/+ mice ( †† p = 0.001), which was blocked by Nr4a2 siRNA treatment ( n = 9–10 per group; ** p

    Article Snippet: This distinct neural circuitry for the ORM and OLM tasks can reveal the specificity of our treatment. shows that, after subthreshold training (3 min), both HDAC3flox/flox ( n = 8) and HDAC3 +/+ ( n = 8) mice spent similar amounts of time with both the familiar and novel objects on test day ( t (14) = 0.40; p = 0.70).

    Techniques: Mouse Assay

    c-fos and Nr4a2 expression are increased in the area of focal homozygous deletion of Hdac3 in HDAC3 flox/flox mice. A , Mice received subthreshold training (3 min) in an environment with two identical objects. B , Two hours after training, quantitative RT-PCR shows that c-fos expression is significantly increased in the dorsal hippocampus of HDAC3 flox/flox mice compared with wild-type littermates ( n = 3 per group; ** p

    Journal: The Journal of neuroscience : the official journal of the Society for Neuroscience

    Article Title: HDAC3 Is a Critical Negative Regulator of Long-Term Memory Formation

    doi: 10.1523/JNEUROSCI.5052-10.2011

    Figure Lengend Snippet: c-fos and Nr4a2 expression are increased in the area of focal homozygous deletion of Hdac3 in HDAC3 flox/flox mice. A , Mice received subthreshold training (3 min) in an environment with two identical objects. B , Two hours after training, quantitative RT-PCR shows that c-fos expression is significantly increased in the dorsal hippocampus of HDAC3 flox/flox mice compared with wild-type littermates ( n = 3 per group; ** p

    Article Snippet: This distinct neural circuitry for the ORM and OLM tasks can reveal the specificity of our treatment. shows that, after subthreshold training (3 min), both HDAC3flox/flox ( n = 8) and HDAC3 +/+ ( n = 8) mice spent similar amounts of time with both the familiar and novel objects on test day ( t (14) = 0.40; p = 0.70).

    Techniques: Expressing, Mouse Assay, Quantitative RT-PCR

    Focal homozygous gene deletion of Hdac3 in the dorsal hippocampus leads to enhanced memory for object location (OLM), which persists at least 7 d but not for object recognition (ORM). A , Mice received subthreshold training (3 min) in an environment with two identical objects and received a retention test 24 h or 7 d later in which one object is moved to a new location. Schematic describes methods for B and C . B , HDAC3 flox/flox mice exhibited significant long-term memory for object location 24 h after subthreshold training ( n = 8 per group; ** p

    Journal: The Journal of neuroscience : the official journal of the Society for Neuroscience

    Article Title: HDAC3 Is a Critical Negative Regulator of Long-Term Memory Formation

    doi: 10.1523/JNEUROSCI.5052-10.2011

    Figure Lengend Snippet: Focal homozygous gene deletion of Hdac3 in the dorsal hippocampus leads to enhanced memory for object location (OLM), which persists at least 7 d but not for object recognition (ORM). A , Mice received subthreshold training (3 min) in an environment with two identical objects and received a retention test 24 h or 7 d later in which one object is moved to a new location. Schematic describes methods for B and C . B , HDAC3 flox/flox mice exhibited significant long-term memory for object location 24 h after subthreshold training ( n = 8 per group; ** p

    Article Snippet: This distinct neural circuitry for the ORM and OLM tasks can reveal the specificity of our treatment. shows that, after subthreshold training (3 min), both HDAC3flox/flox ( n = 8) and HDAC3 +/+ ( n = 8) mice spent similar amounts of time with both the familiar and novel objects on test day ( t (14) = 0.40; p = 0.70).

    Techniques: Mouse Assay

    Loss of HDAC3/NCoR interaction enhances long-term OLM and ORM formation but has no effect on short-term memory. A , Mice received subthreshold training (3 min) in an environment with two identical objects and received a retention test 90 min ( B ) or 24 h ( C ) later in which one object is moved to a new location. B , Subthreshold training did not result in significant short-term memory by either genotype when tested 90 min later ( n = 11–13 per group). C , However, DADm mice showed a significant preference for the novel object location 24 h after training compared with wild types ( n = 9–10 per group; ** p

    Journal: The Journal of neuroscience : the official journal of the Society for Neuroscience

    Article Title: HDAC3 Is a Critical Negative Regulator of Long-Term Memory Formation

    doi: 10.1523/JNEUROSCI.5052-10.2011

    Figure Lengend Snippet: Loss of HDAC3/NCoR interaction enhances long-term OLM and ORM formation but has no effect on short-term memory. A , Mice received subthreshold training (3 min) in an environment with two identical objects and received a retention test 90 min ( B ) or 24 h ( C ) later in which one object is moved to a new location. B , Subthreshold training did not result in significant short-term memory by either genotype when tested 90 min later ( n = 11–13 per group). C , However, DADm mice showed a significant preference for the novel object location 24 h after training compared with wild types ( n = 9–10 per group; ** p

    Article Snippet: This distinct neural circuitry for the ORM and OLM tasks can reveal the specificity of our treatment. shows that, after subthreshold training (3 min), both HDAC3flox/flox ( n = 8) and HDAC3 +/+ ( n = 8) mice spent similar amounts of time with both the familiar and novel objects on test day ( t (14) = 0.40; p = 0.70).

    Techniques: Mouse Assay

    Intrahippocampal AAV2/1–Cre infusion in HDAC3 flox/flox mice results in a complete, focal deletion of HDAC3 that correlates with increased histone acetylation. Images are 4×, except the right panels, which are 20× magnifications of the regions boxed in white. Histograms depict quantification of optical density as a percentage of wild type. A , Representative images showing DAPI labeling and HDAC3 immunoreactivity in hippocampi of AAV2/1–Cre infused HDAC3 +/+ and HDAC3 flox/flox mice. HDAC3 labeling is found throughout CA1, CA3, and the dentate gyrus, and no immunoreactivity is found in the AAV2/1–Cre infusion site of HDAC3 flox/flox mice. * p

    Journal: The Journal of neuroscience : the official journal of the Society for Neuroscience

    Article Title: HDAC3 Is a Critical Negative Regulator of Long-Term Memory Formation

    doi: 10.1523/JNEUROSCI.5052-10.2011

    Figure Lengend Snippet: Intrahippocampal AAV2/1–Cre infusion in HDAC3 flox/flox mice results in a complete, focal deletion of HDAC3 that correlates with increased histone acetylation. Images are 4×, except the right panels, which are 20× magnifications of the regions boxed in white. Histograms depict quantification of optical density as a percentage of wild type. A , Representative images showing DAPI labeling and HDAC3 immunoreactivity in hippocampi of AAV2/1–Cre infused HDAC3 +/+ and HDAC3 flox/flox mice. HDAC3 labeling is found throughout CA1, CA3, and the dentate gyrus, and no immunoreactivity is found in the AAV2/1–Cre infusion site of HDAC3 flox/flox mice. * p

    Article Snippet: This distinct neural circuitry for the ORM and OLM tasks can reveal the specificity of our treatment. shows that, after subthreshold training (3 min), both HDAC3flox/flox ( n = 8) and HDAC3 +/+ ( n = 8) mice spent similar amounts of time with both the familiar and novel objects on test day ( t (14) = 0.40; p = 0.70).

    Techniques: Mouse Assay, Labeling

    Histone deacetylases (HDAC) inhibitors regulate cytokine production. Expressions of IFN-γ and TNF-α were detected by quantitative real-time PCR and sandwich ELISA. (A,B) IFN-γ expression by γδ T cells stimulated with HDMAPP, treated with or without HDAC inhibitors sodium valproate (VPA), Trichostatin-A (TSA), and suberoylanilidehydroxamic acid (SAHA) at different concentrations at mRNA and protein levels, respectively. (C,D) Expression of TNF-α in the supernatants collected from HDMAPP stimulated γδ T cells in the presence or absence of HDAC inhibitors VPA, TSA, and SAHA at different concentrations at mRNA and protein levels, respectively. The expression of different m-RNA transcripts was normalized to 18S r-RNA. All the results indicated are mean ± SEM of three independent experiments, where * p

    Journal: Frontiers in Immunology

    Article Title: Checkpoint Blockade Rescues the Repressive Effect of Histone Deacetylases Inhibitors on γδ T Cell Function

    doi: 10.3389/fimmu.2018.01615

    Figure Lengend Snippet: Histone deacetylases (HDAC) inhibitors regulate cytokine production. Expressions of IFN-γ and TNF-α were detected by quantitative real-time PCR and sandwich ELISA. (A,B) IFN-γ expression by γδ T cells stimulated with HDMAPP, treated with or without HDAC inhibitors sodium valproate (VPA), Trichostatin-A (TSA), and suberoylanilidehydroxamic acid (SAHA) at different concentrations at mRNA and protein levels, respectively. (C,D) Expression of TNF-α in the supernatants collected from HDMAPP stimulated γδ T cells in the presence or absence of HDAC inhibitors VPA, TSA, and SAHA at different concentrations at mRNA and protein levels, respectively. The expression of different m-RNA transcripts was normalized to 18S r-RNA. All the results indicated are mean ± SEM of three independent experiments, where * p

    Article Snippet: Briefly, 0.1 × 106 γδ T cells, seeded in 96-well flat bottom plates (Nunc), were treated with the following HDAC inhibitors for the given concentration range: VPA (4–0.25 mM; Sigma-Aldrich), TSA (200–25 nM; Sigma-Aldrich), and SAHA (4–0.25 µM; Sigma-Aldrich) along with HDMAPP (1 nM; Echelon) and rIL-2 (50 IU/ml; Peprotech) for 72 h. γδ T cells treated only with HDMAPP (1 nM) and rIL-2 (50 IU/ml) were used as control.

    Techniques: Real-time Polymerase Chain Reaction, Sandwich ELISA, Expressing

    Histone deacetylases (HDAC) inhibitors increase the expression of cell cycle checkpoint proteins p53 and p21. Protein expression of p53 and p21 by γδ T cells upon treatment with (A) sodium valproate (VPA), (B) Trichostatin-A (TSA), and (C) suberoylanilidehydroxamic acid (SAHA) as detected by western blotting. Cell lysates of γδ T cells, stimulated with HDMAPP after treatment with HDAC inhibitors at different concentrations for 72 h were prepared and p53, p21 proteins were detected. β-actin was used as loading control. Densitometry quantification of p53 (D–F) and p21 (G–I) expression in γδ T cells upon treatment with VPA, TSA, and SAHA, relative to β-actin.

    Journal: Frontiers in Immunology

    Article Title: Checkpoint Blockade Rescues the Repressive Effect of Histone Deacetylases Inhibitors on γδ T Cell Function

    doi: 10.3389/fimmu.2018.01615

    Figure Lengend Snippet: Histone deacetylases (HDAC) inhibitors increase the expression of cell cycle checkpoint proteins p53 and p21. Protein expression of p53 and p21 by γδ T cells upon treatment with (A) sodium valproate (VPA), (B) Trichostatin-A (TSA), and (C) suberoylanilidehydroxamic acid (SAHA) as detected by western blotting. Cell lysates of γδ T cells, stimulated with HDMAPP after treatment with HDAC inhibitors at different concentrations for 72 h were prepared and p53, p21 proteins were detected. β-actin was used as loading control. Densitometry quantification of p53 (D–F) and p21 (G–I) expression in γδ T cells upon treatment with VPA, TSA, and SAHA, relative to β-actin.

    Article Snippet: Briefly, 0.1 × 106 γδ T cells, seeded in 96-well flat bottom plates (Nunc), were treated with the following HDAC inhibitors for the given concentration range: VPA (4–0.25 mM; Sigma-Aldrich), TSA (200–25 nM; Sigma-Aldrich), and SAHA (4–0.25 µM; Sigma-Aldrich) along with HDMAPP (1 nM; Echelon) and rIL-2 (50 IU/ml; Peprotech) for 72 h. γδ T cells treated only with HDMAPP (1 nM) and rIL-2 (50 IU/ml) were used as control.

    Techniques: Expressing, Western Blot