Article Title: Fluorescent TAP as a Platform for Virus-Induced Degradation of the Antigenic Peptide Transporter
Figure Lengend Snippet: TAP1-GFP interacts with UL49.5 and the peptide-loading complex. TAP1-HN-GFP (T1-HN) was immunoprecipitated by GFP-Trap from lysates of MJS cells expressing TAP1-HN-GFP and UL49.5 or wt MJS with UL49.5 only. Co-precipitating proteins were analyzed by SDS-PAGE and immunoblotting using antibodies against GFP, TAP2, ERp57, MHC I HC, and UL49.5. Right panel: cell lysates were loaded on SDS-PAGE directly and analyzed by immunoblotting.
Article Snippet: Antibodies used for immunoblotting: mouse anti-TAP1 monoclonal antibody MAb 143.5 (kindly provided by R. Tampé, Institute of Biochemistry, The Johann Wolfgang Goethe University, Frankfurt, Germany); mouse anti-TAP2 MAb 435.3 (a kind gift from P. van Endert, INSERM U25, Institute Necker, Paris, France); rabbit anti-TAP1 (Enzo Life Sciences, Farmingdale, NY, USA); rat anti-GFP 3H9 (Chromotek, Planegg, Germany); mouse anti-myc tag 9B11 (Cell Signaling, Danvers, MA, USA); rabbit anti-β-actin (Novus Biologicals, Centennial, CO, USA); rabbit anti-β-catenin (Santa Cruz Biotechnology, Dallas, TX, USA); rabbit antibodies (H11) against a synthetic peptide derived from the N-terminal domain of BoHV-1 UL49.5 [ ] and mouse anti-OctA (FLAG) G-8 (Santa Cruz Biotechnology); and mouse anti-HC10 [ ] and rabbit anti-ERp57 H-220 (Santa Cruz Biotechnology).
Techniques: Immunoprecipitation, Expressing, SDS Page