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galanthus nivalis lectin gnl  (Vector Laboratories)
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Vector Laboratories galanthus nivalis lectin gnl
Cell wall integrity defect in the ΔMrSkn7 mutant. (A) The growth of the ΔMrSkn7 mutant (top row in each panel) was inhibited on PDA supplemented with 200 μg/ml calcofluor white (CFW) or 250 μg/ml Congo red (CR) for 2 days compared to the WT (bottom row in each panel). (B) Fluorescence staining of cell wall components with the <t>lectin</t> GSII (Griffonia simplicifolia) for chitin binding. (C) Staining with the lectin concanavalin A (ConA) to label the components of α-glucoside and α-mannose. (D) Staining with the lectin <t>GNL</t> <t>(Galanthus</t> nivalis lectin) to label α-mannose. Bars = 5 μm.
Galanthus Nivalis Lectin Gnl, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Cell wall integrity defect in the ΔMrSkn7 mutant. (A) The growth of the ΔMrSkn7 mutant (top row in each panel) was inhibited on PDA supplemented with 200 μg/ml calcofluor white (CFW) or 250 μg/ml Congo red (CR) for 2 days compared to the WT (bottom row in each panel). (B) Fluorescence staining of cell wall components with the lectin GSII (Griffonia simplicifolia) for chitin binding. (C) Staining with the lectin concanavalin A (ConA) to label the components of α-glucoside and α-mannose. (D) Staining with the lectin GNL (Galanthus nivalis lectin) to label α-mannose. Bars = 5 μm.

Journal: Eukaryotic Cell

Article Title: MrSkn7 Controls Sporulation, Cell Wall Integrity, Autolysis, and Virulence in Metarhizium robertsii

doi: 10.1128/EC.00266-14

Figure Lengend Snippet: Cell wall integrity defect in the ΔMrSkn7 mutant. (A) The growth of the ΔMrSkn7 mutant (top row in each panel) was inhibited on PDA supplemented with 200 μg/ml calcofluor white (CFW) or 250 μg/ml Congo red (CR) for 2 days compared to the WT (bottom row in each panel). (B) Fluorescence staining of cell wall components with the lectin GSII (Griffonia simplicifolia) for chitin binding. (C) Staining with the lectin concanavalin A (ConA) to label the components of α-glucoside and α-mannose. (D) Staining with the lectin GNL (Galanthus nivalis lectin) to label α-mannose. Bars = 5 μm.

Article Snippet: These fluorescent lectins include the Alexa Fluor 488-labeled concanavalin A (ConA) (Invitrogen) (60 μg/ml) for binding α-mannopyranosyl and α-glucopyranosyl residues, lectin GSII (from Griffonia simplicifolia ) (Invitrogen) (20 μg/ml) for binding α- or β-linked N -acetyl- d -glucosamine (GlcNAc), and fluorescein-labeled Galanthus nivalis lectin (GNL) (Vector Laboratories) (20 μg/ml) for binding α-1,3-mannose ( 27 ).

Techniques: Mutagenesis, Fluorescence, Staining, Binding Assay