FAB1027J Search Results


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Bio-Techne corporation mouse anti cd276
Yield and enrichment of 80- and 641-amino acid linkers in a <t>CD276</t> system. Yeast displaying either pCT-80 or pCT-641 tethered ligands were mixed with nondisplaying yeast and sorted in parallel by adherent cell panning. (A) Enrichment ratio, (B) ligand-displaying yeast yield and (C) nondisplaying yeast yield were quantified. Ligand: AC2. Cell Line: MDA-MB-231. The dotted line (A) indicates the limit of functional enrichment. *P < 0.05 relative to the 80- amino acid linker
Mouse Anti Cd276, supplied by Bio-Techne corporation, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Yield and enrichment of 80- and 641-amino acid linkers in a CD276 system. Yeast displaying either pCT-80 or pCT-641 tethered ligands were mixed with nondisplaying yeast and sorted in parallel by adherent cell panning. (A) Enrichment ratio, (B) ligand-displaying yeast yield and (C) nondisplaying yeast yield were quantified. Ligand: AC2. Cell Line: MDA-MB-231. The dotted line (A) indicates the limit of functional enrichment. *P < 0.05 relative to the 80- amino acid linker

Journal: Protein Engineering, Design and Selection

Article Title: Extended yeast surface display linkers enhance the enrichment of ligands in direct mammalian cell selections

doi: 10.1093/protein/gzab004

Figure Lengend Snippet: Yield and enrichment of 80- and 641-amino acid linkers in a CD276 system. Yeast displaying either pCT-80 or pCT-641 tethered ligands were mixed with nondisplaying yeast and sorted in parallel by adherent cell panning. (A) Enrichment ratio, (B) ligand-displaying yeast yield and (C) nondisplaying yeast yield were quantified. Ligand: AC2. Cell Line: MDA-MB-231. The dotted line (A) indicates the limit of functional enrichment. *P < 0.05 relative to the 80- amino acid linker

Article Snippet: Beads and cells were separately labeled with either mouse anti-EGFR clone ab30 (4 μg/ml) (Abcam), mouse anti-CD276 clone 185504 (4 μg/ml) (Biotechne) or anti-c-Myc clone 9E10 (4 μg/mL) (BioLegend) for 30 min at 4°C.

Techniques: Functional Assay