F53765 Search Results


mouse osteocalcin-elisa kit  (Absolute Biotech Inc)
90
Absolute Biotech Inc mouse osteocalcin-elisa kit
R-spondin-2 null mesenchymal progenitor cells from Footless mutant mice show reduced osteoblastogenesis. a Alizarin red S staining of representative wells from in vitro osteogenesis assay after 10 days. b Overall quantification of Alizarin Red S staining of osteogenesis at 10 days, as shown in a . c Quantification of osteogenesis at 15 days with addition of recombinant hRSPO2 (10 nmol·L -1 ). d Alkaline Phosphatase (ALP) staining of similar osteogenesis assay at 5 days. e Relative Runx2 expression after 5 days of osteogenesis in vitro. f Relative Osterix expression after 5 days of osteogenesis in vitro. g Relative <t>Osteocalcin</t> expression after 10 days of osteogenesis in vitro. h Relative bone sialoprotein (BSP) expression after 5 days of osteogenesis in vitro. ( n = 3 cell lines from individual animals, assay performed in triplicate for each cell line (sex of pre-natal pups not determined), * indicates P < 0.05. MM maintenance media, OPM osteopermissive media with β-glycerophosphate and ascorbic acid-2-phosphate, OGM osteogenic media with OPM and 2.5 nmol·L -1 rhBMP-6
Mouse Osteocalcin Elisa Kit, supplied by Absolute Biotech Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/mouse osteocalcin-elisa kit/product/Absolute Biotech Inc
Average 90 stars, based on 1 article reviews
mouse osteocalcin-elisa kit - by Bioz Stars, 2026-03
90/100 stars
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runx2 antibody  (NSJ Bioreagents)
N/A
Runx2 is a member of the RUNX family of transcription factors. It is a nuclear protein with an Runt DNA-binding domain. This protein is essential for osteoblastic differentiation and skeletal morphogenesis and acts as a
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mouse plexin c1 biotinylated antibody  (R&D Systems)
N/A
The Mouse Plexin C1 Biotinylated Antibody from R&D Systems is a Plexin C1 antibody to Plexin C1. This antibody reacts with Mouse. The Plexin C1 antibody has been validated for the following applications: Western Blot.
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tert-butyl (6s)-6-hydroxy-1,4-oxazepane-4-carboxylate  (Aladdin Scientific Corporation)
N/A
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aurkc antibody  (NSJ Bioreagents)
N/A
Chromosomal segregation during mitosis as well as meiosis is regulated by kinases and phosphatases. The Aurora kinases, members of the Ser/Thr protein kinase family, associate with microtubules during chromosome movement and segregation. Aurora kinase C
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mouse cd55/daf biotinylated antibody  (R&D Systems)
N/A
The Mouse CD55/DAF Biotinylated Antibody from R&D Systems is a CD55/DAF antibody to CD55/DAF. This antibody reacts with Mouse. The CD55/DAF antibody has been validated for the following applications: Western Blot.
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mouse plexin c1 antibody  (R&D Systems)
N/A
The Mouse Plexin C1 Antibody from R&D Systems is a Plexin C1 antibody to Plexin C1. This antibody reacts with Mouse. The Plexin C1 antibody has been validated for the following applications: Western Blot, Flow
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bmpr2 antibody  (NSJ Bioreagents)
N/A
BMPR2 is a member of the bone morphogenetic protein (BMP) receptor family of transmembrane serine/threonine kinases. The ligands of this receptor are BMPs, which are members of the TGF-beta superfamily. BMPs are involved in endochondral
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phospho-tsc2 antibody  (NSJ Bioreagents)
N/A
Mutations in TSC2 lead to tuberous sclerosis complex. The protein is believed to be a tumor suppressor and is able to specifically stimulate the intrinsic GTPase activity of the Ras-related protein RAP1A and RAB5. The
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mouse cd55 daf antibody  (R&D Systems)
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The Mouse CD55 DAF Antibody from R D Systems is a sheep polyclonal antibody to CD55 DAF This antibody reacts with mouse The Mouse CD55 DAF Antibody has been validated for the following applications Western
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irak4 antibody  (NSJ Bioreagents)
N/A
Protein kinases are enzymes that transfer a phosphate group from a phosphate donor, generally the g phosphate of ATP, onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate
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Image Search Results


R-spondin-2 null mesenchymal progenitor cells from Footless mutant mice show reduced osteoblastogenesis. a Alizarin red S staining of representative wells from in vitro osteogenesis assay after 10 days. b Overall quantification of Alizarin Red S staining of osteogenesis at 10 days, as shown in a . c Quantification of osteogenesis at 15 days with addition of recombinant hRSPO2 (10 nmol·L -1 ). d Alkaline Phosphatase (ALP) staining of similar osteogenesis assay at 5 days. e Relative Runx2 expression after 5 days of osteogenesis in vitro. f Relative Osterix expression after 5 days of osteogenesis in vitro. g Relative Osteocalcin expression after 10 days of osteogenesis in vitro. h Relative bone sialoprotein (BSP) expression after 5 days of osteogenesis in vitro. ( n = 3 cell lines from individual animals, assay performed in triplicate for each cell line (sex of pre-natal pups not determined), * indicates P < 0.05. MM maintenance media, OPM osteopermissive media with β-glycerophosphate and ascorbic acid-2-phosphate, OGM osteogenic media with OPM and 2.5 nmol·L -1 rhBMP-6

Journal: Bone Research

Article Title: R-spondin-2 is a Wnt agonist that regulates osteoblast activity and bone mass

doi: 10.1038/s41413-018-0026-7

Figure Lengend Snippet: R-spondin-2 null mesenchymal progenitor cells from Footless mutant mice show reduced osteoblastogenesis. a Alizarin red S staining of representative wells from in vitro osteogenesis assay after 10 days. b Overall quantification of Alizarin Red S staining of osteogenesis at 10 days, as shown in a . c Quantification of osteogenesis at 15 days with addition of recombinant hRSPO2 (10 nmol·L -1 ). d Alkaline Phosphatase (ALP) staining of similar osteogenesis assay at 5 days. e Relative Runx2 expression after 5 days of osteogenesis in vitro. f Relative Osterix expression after 5 days of osteogenesis in vitro. g Relative Osteocalcin expression after 10 days of osteogenesis in vitro. h Relative bone sialoprotein (BSP) expression after 5 days of osteogenesis in vitro. ( n = 3 cell lines from individual animals, assay performed in triplicate for each cell line (sex of pre-natal pups not determined), * indicates P < 0.05. MM maintenance media, OPM osteopermissive media with β-glycerophosphate and ascorbic acid-2-phosphate, OGM osteogenic media with OPM and 2.5 nmol·L -1 rhBMP-6

Article Snippet: Osteocalcin (Mouse osteocalcin-ELISA kit Cat# LS-F5375 LSBio, USA) and TRAP5b (MouseTRAP™ (TRAcP 5b) ELISA Cat# SB-TR103 IDS, immunodiagnosticsystems, USA) in serum of WT and Ocn-Cre + Rspo2 f/f 3- and 6-month-old mice were quantified using commercially available kits.

Techniques: Mutagenesis, Staining, In Vitro, Recombinant, Expressing