F53573 Search Results


ret  (NSJ Bioreagents)
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NSJ Bioreagents ret
( A ) PLA of endogenous CacyBP and <t>RET</t> in human skin organoids generated <t>from</t> <t>MAB21L4</t> -ablated primary keratinocytes (sg MAB21L4 ) compared to control sgRNA. Quantitation of PLA index with two independent MAB21L4 sgRNAs. ( B ) Western blot analysis of SCCIC1 cells subjected to RET or IgG co-immunoprecipitation. Data shown is representative of n=3 technical replicates. ( C ) Dissociation curve of recombinant purified CacyBP and RET proteins in the presence or absence of MAB21L4 measured by MST. Data show technical triplicates and are representative of results obtained in two independent experiments. PLA of endogenous Siah1 and RET in CACYBP -ablated human skin organoids ( D ); endogenous ubiquitin and RET in CACYBP -ablated ( E ), MAB21L4 -ablated ( F ), and SIAH1 -ablated ( G ) human skin organoids. In each case, quantitation of PLA index with two independent sgRNAs is shown. All organoids were grown for three days on dermis and results are representative of at least two independent experiments. All scale bars, 100 μm. PLA index was calculated from three fields of view using the number of dots per nucleus. Data represent mean ± s.d.; statistical significance was determined by two-tailed t-test.
Ret, supplied by NSJ Bioreagents, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/ret/product/NSJ Bioreagents
Average 92 stars, based on 1 article reviews
ret - by Bioz Stars, 2026-02
92/100 stars
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human carbohydrate sulfotransferase 4/ chst4 biotinylated antibody  (R&D Systems)
N/A
The Human Carbohydrate Sulfotransferase 4/ CHST4 Biotinylated Antibody from R&D Systems is a Carbohydrate Sulfotransferase 4/CHST4 antibody to Carbohydrate Sulfotransferase 4/CHST4. This antibody reacts with Human. The Carbohydrate Sulfotransferase 4/CHST4 antibody has been validated for
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sox9 antibody  (NSJ Bioreagents)
N/A
The protein encoded by this gene recognizes the sequence CCTTGAG along with other members of the HMG-box class DNA-binding proteins. It acts during chondrocyte differentiation and, with steroidogenic factor 1, regulates transcription of the anti-Muellerian
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cd10 antibody  (NSJ Bioreagents)
N/A
Thermolysin-like specificity, but is almost confined on acting on polypeptides of up to 30 amino acids. Biologically important in the destruction of opioid peptides such as Met- and Leu-enkephalins by cleavage of a Gly-Phe bond.
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2-(fmoc-amino)-5-(cyclopropylmethoxy)benzoic acid  (Biosynth Carbosynth)
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rnf8 antibody  (NSJ Bioreagents)
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The protein encoded by this gene contains a RING finger motif and a FHA domain. This protein has been shown to interact with several class II ubiquitin-conjugating enzymes (E2), including UBE2E1/UBCH6, UBE2E2, and UBE2E3, and
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glutaryl chloride  (Chem Impex International)
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human carbohydrate sulfotransferase 4/chst4 antibody  (R&D Systems)
N/A
The Human Carbohydrate Sulfotransferase 4/CHST4 Antibody from R&D Systems is a Carbohydrate Sulfotransferase 4/CHST4 antibody to Carbohydrate Sulfotransferase 4/CHST4. This antibody reacts with Human. The Carbohydrate Sulfotransferase 4/CHST4 antibody has been validated for the following
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human carbohydrate sulfotransferase 4 chst4 antibody  (R&D Systems)
N/A
The Human Carbohydrate Sulfotransferase 4 CHST4 Antibody from R D Systems is a sheep polyclonal antibody to Carbohydrate Sulfotransferase 4 CHST4 This antibody reacts with human The Human Carbohydrate Sulfotransferase 4 CHST4 Antibody has been
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Image Search Results


( A ) PLA of endogenous CacyBP and RET in human skin organoids generated from MAB21L4 -ablated primary keratinocytes (sg MAB21L4 ) compared to control sgRNA. Quantitation of PLA index with two independent MAB21L4 sgRNAs. ( B ) Western blot analysis of SCCIC1 cells subjected to RET or IgG co-immunoprecipitation. Data shown is representative of n=3 technical replicates. ( C ) Dissociation curve of recombinant purified CacyBP and RET proteins in the presence or absence of MAB21L4 measured by MST. Data show technical triplicates and are representative of results obtained in two independent experiments. PLA of endogenous Siah1 and RET in CACYBP -ablated human skin organoids ( D ); endogenous ubiquitin and RET in CACYBP -ablated ( E ), MAB21L4 -ablated ( F ), and SIAH1 -ablated ( G ) human skin organoids. In each case, quantitation of PLA index with two independent sgRNAs is shown. All organoids were grown for three days on dermis and results are representative of at least two independent experiments. All scale bars, 100 μm. PLA index was calculated from three fields of view using the number of dots per nucleus. Data represent mean ± s.d.; statistical significance was determined by two-tailed t-test.

Journal: bioRxiv

Article Title: RET activation controlled by MAB21L4-CacyBP interaction drives squamous cell carcinoma

doi: 10.1101/2022.01.19.476979

Figure Lengend Snippet: ( A ) PLA of endogenous CacyBP and RET in human skin organoids generated from MAB21L4 -ablated primary keratinocytes (sg MAB21L4 ) compared to control sgRNA. Quantitation of PLA index with two independent MAB21L4 sgRNAs. ( B ) Western blot analysis of SCCIC1 cells subjected to RET or IgG co-immunoprecipitation. Data shown is representative of n=3 technical replicates. ( C ) Dissociation curve of recombinant purified CacyBP and RET proteins in the presence or absence of MAB21L4 measured by MST. Data show technical triplicates and are representative of results obtained in two independent experiments. PLA of endogenous Siah1 and RET in CACYBP -ablated human skin organoids ( D ); endogenous ubiquitin and RET in CACYBP -ablated ( E ), MAB21L4 -ablated ( F ), and SIAH1 -ablated ( G ) human skin organoids. In each case, quantitation of PLA index with two independent sgRNAs is shown. All organoids were grown for three days on dermis and results are representative of at least two independent experiments. All scale bars, 100 μm. PLA index was calculated from three fields of view using the number of dots per nucleus. Data represent mean ± s.d.; statistical significance was determined by two-tailed t-test.

Article Snippet: Antigen retrieval was performed in 0.01 M Na Citrate (pH 6) and the following primary antibodies were used: MAB21L4 (1:500, Biorbyt, orb159238), RET (1:200, NSJ Bioreagents, F53523), KRT1 (1:200, Biolegend, 905201), LOR (1:200, Biolegend, PRB-145P-100).

Techniques: Generated, Quantitation Assay, Western Blot, Immunoprecipitation, Recombinant, Purification, Two Tailed Test

( A ) Increased mRNA expression of RET as well as DUSP6 and SPRY4 , two biomarkers of RET activity, in cSCC compared to normal skin. P -values were calculated using DESeq2. ( B ) cSCC-associated RET mutations are plotted as a function of CADD score and compared to known oncogenic mutations. CLD, cadherin-like domain. Cys, cysteine-rich domain. TK, tyrosine kinase. ( C ) Differentiation gene mRNA induction in human skin organoids generated using two independent RET sgRNAs compared to control sgRNA and after three days of growth on dermis. ( D ) Relative expression of differentiation genes in human skin organoids grown for three days on dermis in the presence or absence of BLU-667 (15 nM). ( E ) Keratin 5 (red) and collagen VII (green) immunofluorescence of oncogenic Ras-transformed human skin organoids generated from RET -ablated human primary keratinocytes (sg RET ) or control sgRNA after 7 days of growth on dermis. Quantitation of invasion index using four fields of view from each of two independent RET sgRNAs. ( F ) Keratin 5 (red) and collagen VII (green) immunofluorescence of oncogenic Ras-transformed human skin organoids generated from MAB21L4 -ablated human primary keratinocytes (sg MAB21L4 ) or control sgRNA after 7 days of growth on dermis; tissue was regenerated in the presence or absence of BLU-667, a highly selective RET inhibitor. Quantitation of invasion index in using four fields of view from each of two independent MAB21L4 sgRNAs. ( G ) Keratin 5 (red) and collagen VII (green) immunofluorescence of oncogenic Ras-transformed human skin organoids after 7 or 14 days of growth on dermis; BLU-667 or DMSO was initiated on day 7. Quantitation of invasion index and invasion depth using at least three fields of view. Data in E , F , and G are shown as mean ± s.d. and are representative of at least two independent experiments. The invasion index is calculated from the integrated density of keratin 5 staining beneath the basement membrane divided by the length of the basement membrane. The invasion depth is measured as the depth of the tumor front below the basement membrane Bars in C - D represent the mean of three technical replicates ± s.d. and similar results were confirmed in two independent experiments. Statistical significance was determined by two-tailed t-test. All scale bars, 100 μm.

Journal: bioRxiv

Article Title: RET activation controlled by MAB21L4-CacyBP interaction drives squamous cell carcinoma

doi: 10.1101/2022.01.19.476979

Figure Lengend Snippet: ( A ) Increased mRNA expression of RET as well as DUSP6 and SPRY4 , two biomarkers of RET activity, in cSCC compared to normal skin. P -values were calculated using DESeq2. ( B ) cSCC-associated RET mutations are plotted as a function of CADD score and compared to known oncogenic mutations. CLD, cadherin-like domain. Cys, cysteine-rich domain. TK, tyrosine kinase. ( C ) Differentiation gene mRNA induction in human skin organoids generated using two independent RET sgRNAs compared to control sgRNA and after three days of growth on dermis. ( D ) Relative expression of differentiation genes in human skin organoids grown for three days on dermis in the presence or absence of BLU-667 (15 nM). ( E ) Keratin 5 (red) and collagen VII (green) immunofluorescence of oncogenic Ras-transformed human skin organoids generated from RET -ablated human primary keratinocytes (sg RET ) or control sgRNA after 7 days of growth on dermis. Quantitation of invasion index using four fields of view from each of two independent RET sgRNAs. ( F ) Keratin 5 (red) and collagen VII (green) immunofluorescence of oncogenic Ras-transformed human skin organoids generated from MAB21L4 -ablated human primary keratinocytes (sg MAB21L4 ) or control sgRNA after 7 days of growth on dermis; tissue was regenerated in the presence or absence of BLU-667, a highly selective RET inhibitor. Quantitation of invasion index in using four fields of view from each of two independent MAB21L4 sgRNAs. ( G ) Keratin 5 (red) and collagen VII (green) immunofluorescence of oncogenic Ras-transformed human skin organoids after 7 or 14 days of growth on dermis; BLU-667 or DMSO was initiated on day 7. Quantitation of invasion index and invasion depth using at least three fields of view. Data in E , F , and G are shown as mean ± s.d. and are representative of at least two independent experiments. The invasion index is calculated from the integrated density of keratin 5 staining beneath the basement membrane divided by the length of the basement membrane. The invasion depth is measured as the depth of the tumor front below the basement membrane Bars in C - D represent the mean of three technical replicates ± s.d. and similar results were confirmed in two independent experiments. Statistical significance was determined by two-tailed t-test. All scale bars, 100 μm.

Article Snippet: Antigen retrieval was performed in 0.01 M Na Citrate (pH 6) and the following primary antibodies were used: MAB21L4 (1:500, Biorbyt, orb159238), RET (1:200, NSJ Bioreagents, F53523), KRT1 (1:200, Biolegend, 905201), LOR (1:200, Biolegend, PRB-145P-100).

Techniques: Expressing, Activity Assay, Generated, Immunofluorescence, Transformation Assay, Quantitation Assay, Staining, Two Tailed Test

Proposed model: Loss of MAB21L4-CacyBP interaction in cSCC enables sustained RET activation and disrupts the balance between proliferation and differentiation.

Journal: bioRxiv

Article Title: RET activation controlled by MAB21L4-CacyBP interaction drives squamous cell carcinoma

doi: 10.1101/2022.01.19.476979

Figure Lengend Snippet: Proposed model: Loss of MAB21L4-CacyBP interaction in cSCC enables sustained RET activation and disrupts the balance between proliferation and differentiation.

Article Snippet: Antigen retrieval was performed in 0.01 M Na Citrate (pH 6) and the following primary antibodies were used: MAB21L4 (1:500, Biorbyt, orb159238), RET (1:200, NSJ Bioreagents, F53523), KRT1 (1:200, Biolegend, 905201), LOR (1:200, Biolegend, PRB-145P-100).

Techniques: Activation Assay