Journal: Scientific Reports

Article Title: HMGB1 induction of clusterin creates a chemoresistant niche in human prostate tumor cells

doi: 10.1038/srep15085

Figure Lengend Snippet: ( A ) DU145 prostate tumor cells, incubated with recombinant human HMGB1 (rhHMGB1) for 24–48 h, were lysed and analyzed by western blot for presence of clusterin, and for β-actin for equal loading. ( B ) DU145 tumor cells were treated with docetaxel (DTX) for 1–4 days and their supernatants were evaluated for HMGB1 by ELISA. ( C ) DU145 tumor cells were treated with the indicated chemotherapeutic agents for 24 h and the supernatants analyzed for HMGB1.

Article Snippet: DU145 cells or PC3 cells were treated with docetaxel (DTX) for 1–4 days or were treated with the indicated chemotherapeutic agents for 24 h. Supernatants were collected and analyzed for the presence of human HMGB1 by an ELISA kit (LS-F4038) from LifeSpan Biosciences.

Techniques: Incubation, Recombinant, Western Blot, Enzyme-linked Immunosorbent Assay

Journal: Polymers

Article Title: Reinforced Universal Adhesive by Ribose Crosslinker: A Novel Strategy in Adhesive Dentistry

doi: 10.3390/polym13050704

Figure Lengend Snippet: Mean ± SD of micro-tensile bond strength (μTBS) in MPa in different groups after 24 h. In the rows, groups identified by different symbols are statistically different ( p < 0.05). μTBS: micro-tensile bond strength. Mean ± SD for inhibition of MMP-2 and cathepsin-K activities 7 and 14 days. Comparison of MMP-2 and cathepsin-K activities (ng/mL) obtained with Human MMP-2 and cathepsin-K ELISA Kit system. Groups identified by different superscripts letter are statistically significantly different within each column. Mean ± SD of contact angle in different groups after treating with different concentrations of RB. Groups identified by different superscripts capital letters for different groups (shows differences between variables) are statistically different within each column.

Article Snippet: ELISA (Human MMP-2 ELISA Kit, or Human CTSK/cathepsin-K ELISA Kit; Lifespan Biosciences, Seattle, WA, USA) was used to quantify the concentrations of MMP-2 and cathepsin-K in collected supernatants derived from the four groups at 7 and 14 days.

Techniques: Inhibition, Enzyme-linked Immunosorbent Assay

Journal: BMC Cancer

Article Title: Translational control of Bcl-2 promotes apoptosis of gastric carcinoma cells

doi: 10.1186/s12885-020-07711-6

Figure Lengend Snippet: Altered miR-383 and Bcl-2 inversely correlates in GC specimens. RT-qPCR on miR-383 and ELISA for Bcl-2 were performed on paired GC and the adjacent non-tumor gastric tissue (NT) from 50 patients. a-b Bcl-2 levels, shown by individual values ( a ) and by mean ± SD ( b ). c-d miR-383 levels, shown by individual values ( c ) and by mean ± SD ( d ). For b and d , values were normalized to NT (=1). e ELISA for Bcl-2 in GC with or without LN metastasis. f RT-qPCR for miR-383 in GC with or without LN metastasis. For E and F, values were normalized to GC without LN metastasis (=1). g A Correlation test between relative Bcl-2 and miR-383 levels. Mean level was set up as 50. h A Correlation test between relative Bcl-2 and cleavage Casp3 levels. Mean level was set up as 50. i Kaplan-Meier curve for overall survival. * p < 0.05. ** p < 0.01. N = 50

Article Snippet: The ELISA for Bcl-2 and cleavage caspase 3 (Casp3) were performed using a human Bcl-2 ELISA kit (LS-F4134, LSbio Lifespan Biosciences Inc., Seattle, WA, USA) or a human cleavage Casp3 ELISA kit (KM300, R&D Biosystem, Beijing, China).

Techniques: Quantitative RT-PCR, Enzyme-linked Immunosorbent Assay

Journal: BMC Cancer

Article Title: Translational control of Bcl-2 promotes apoptosis of gastric carcinoma cells

doi: 10.1186/s12885-020-07711-6

Figure Lengend Snippet: MiR-383 inhibits translation of Bcl-2 mRNA via 3′-UTR binding. a Bioinformatic prediction. b-c Overexpression or depletion of miR-383 in 2 human GC cell lines, AGS ( b ) and SNU-5 ( c ), by transfection with a miR-383-mimic, or with a plasmid carrying miR-383 antisense. Transfection with a null plasmid was used as a control. RT-qPCR was performed to assess miR-383 levels. For B and C, values were normalized to null-transfected cells (=1). (D-E) MiR-383-modified AGS ( d ) or SNU-5 ( e ) cells were transfected with wildtype or mutant Bcl-2-3’UTR for a luciferase reporter assay. For D and E, values were normalized to null only -transfected cells (=1). * p < 0.05. N = 5 (each experimental group contains 5 repeats)

Article Snippet: The ELISA for Bcl-2 and cleavage caspase 3 (Casp3) were performed using a human Bcl-2 ELISA kit (LS-F4134, LSbio Lifespan Biosciences Inc., Seattle, WA, USA) or a human cleavage Casp3 ELISA kit (KM300, R&D Biosystem, Beijing, China).

Techniques: Binding Assay, Over Expression, Transfection, Plasmid Preparation, Quantitative RT-PCR, Modification, Mutagenesis, Luciferase, Reporter Assay

Journal: BMC Cancer

Article Title: Translational control of Bcl-2 promotes apoptosis of gastric carcinoma cells

doi: 10.1186/s12885-020-07711-6

Figure Lengend Snippet: Post-transcriptional control of miR-383 on Bcl-2. a-b RT-qPCR for assessing Bcl-2 mRNA levels in miR-383-modified AGS cells ( a ) and miR-383-modified SNU-5 cells ( b ). c-d ELISA for Bcl-2 protein levels in miR-383-modified AGS cells ( c ) and miR-383-modified SNU-5 cells ( d ). Values were normalized to null-transfected cells (=1). NS: non-significant. *p < 0.05. N = 5 (each experimental group contains 5 repeats)

Article Snippet: The ELISA for Bcl-2 and cleavage caspase 3 (Casp3) were performed using a human Bcl-2 ELISA kit (LS-F4134, LSbio Lifespan Biosciences Inc., Seattle, WA, USA) or a human cleavage Casp3 ELISA kit (KM300, R&D Biosystem, Beijing, China).

Techniques: Quantitative RT-PCR, Modification, Enzyme-linked Immunosorbent Assay, Transfection

Journal: BMC Cancer

Article Title: Translational control of Bcl-2 promotes apoptosis of gastric carcinoma cells

doi: 10.1186/s12885-020-07711-6

Figure Lengend Snippet: Bioinformatics analyses confirm the regulatory axis of miR-383/Bcl-2 in GC. a-b Volcano image ( a ) and survival curve for miR-383 ( b ) for GEO database GSE107754, GPL4133, GPL570 and GPL5639 to compare gene profiling of GC cells to NT cells. Data were analyzed by R language. *p < 0.05. N = 463

Article Snippet: The ELISA for Bcl-2 and cleavage caspase 3 (Casp3) were performed using a human Bcl-2 ELISA kit (LS-F4134, LSbio Lifespan Biosciences Inc., Seattle, WA, USA) or a human cleavage Casp3 ELISA kit (KM300, R&D Biosystem, Beijing, China).

Techniques: