Journal: The Journal of Experimental Medicine

Article Title: Integrin α M β 2 -Mediated Cell Migration to Fibrinogen and Its Recognition Peptides

doi:

Figure Lengend Snippet: Migration of α M β 2 WT transfectants but not mock transfectants to Fg. Human 293 cells (5 × 10 5 /well) expressing α M β 2 (WT, black bars) or mock-transfected cells containing only the neomycin resistance plasmid (white bars) were assessed for their ability to migrate to Fg (50 μg/ml, 150 nM), Fn (50 μg/ml), or medium alone placed in the lower wells of transwell plates. Some cells were pretreated with 20 μg/ml anti-β 1 blocking mAb F4611 (A) or 100 nM NIF; M1/70 and 44a, 20 μg/ml α M -blocking mAb; IB4, 20 μg/ml β 2 -blocking mAb; or LM2/1, 20 μg/ml α M -nonblocking mAb (B) for 30 min before addition to the upper wells. Migration was assessed for 22 h at 37°C and migrated cells were fixed, stained, and counted. Migration data are expressed as mean cells/HPF ± SD for five random fields per well (A) or as a percentage of the migration of the WT cells to Fg (B) with duplicate wells in each experiment from three or more experiments. The x-axis indicates the addition to the upper wells containing cells over the addition to the lower wells. ***Medium alone.

Article Snippet: The anti-β 1 mAb F4611 and anti-α V β 3 mAb LM609 were purchased from Chemicon.

Techniques: Migration, Expressing, Transfection, Plasmid Preparation, Blocking Assay, Staining