Journal: Neural Regeneration Research

Article Title: Repairing whole facial nerve defects with xenogeneic acellular nerve grafts in rhesus monkeys

doi: 10.4103/1673-5374.324853

Figure Lengend Snippet: Morphological assessment at 8 months postoperatively . The Retrograde Labeling Test (original magnification, 400×; A, B) revealed FluoroGold-labeled neurons with multipolar dendrites in the facial nerve nuclei. Hematoxylin-eosin staining (original magnification, 200×; C, D) and S100 immunohistochemical staining (original magnification, 200×; E, F) showed the regenerated nerve fibers, and S100-positive Schwann cells and myelin sheaths were densely clustered, with different sizes, in the transverse section. Transmission electron micrographs (original magnification, 1200×; G, H) showed that most myelin sheaths were arranged in the mature and uniform lamellar pattern, and that the Schwann cells had perfect basement membranes. Synaptophysin immunohistochemical staining (original magnification, 200×; I, J) of the orbicularis oris muscle showed that some motor endplates were reinnervated in the muscle. (A, C, E, G, I) The autograft group; (B, D, F, H, J) the xenogeneic acellular nerve graft group.

Article Snippet: Immediately after electrophysiological monitoring at 8 months postoperatively, 15 μL of 5% FluoroGold solution (F4040, US EVERBRIGHT®INC., Suzhou, Jiangsu Province, China) was injected at multiple points into the buccal division of the facial nerve, distal to the nerve graft.

Techniques: Labeling, Staining, Immunohistochemical staining, Transmission Assay