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Image Search Results
Journal: Scientific Reports
Article Title: HMGB1 induction of clusterin creates a chemoresistant niche in human prostate tumor cells
doi: 10.1038/srep15085
Figure Lengend Snippet: ( A ) DU145 prostate tumor cells, incubated with recombinant human HMGB1 (rhHMGB1) for 24–48 h, were lysed and analyzed by western blot for presence of clusterin, and for β-actin for equal loading. ( B ) DU145 tumor cells were treated with docetaxel (DTX) for 1–4 days and their supernatants were evaluated for HMGB1 by ELISA. ( C ) DU145 tumor cells were treated with the indicated chemotherapeutic agents for 24 h and the supernatants analyzed for HMGB1.
Article Snippet: DU145 cells or PC3 cells were treated with docetaxel (DTX) for 1–4 days or were treated with the indicated chemotherapeutic agents for 24 h. Supernatants were collected and analyzed for the presence of human HMGB1 by an
Techniques: Incubation, Recombinant, Western Blot, Enzyme-linked Immunosorbent Assay