Journal: Molecular Medicine

Article Title: Chemerin alleviates the placental oxidative stress and improves fetal overgrowth of gestational diabetes mellitus mice induced by high fat diet

doi: 10.1186/s10020-024-01007-2

Figure Lengend Snippet: The effects of different concentrations of chemerin on ROS level, T-AOC and glucose uptake in the high glucose cell model. ( A ) The relative fluorescence intensity of ROS. ( B ) The detection of total antioxidant capacity. ( C ) The intake of glucose by cells. HG: high glucose; ROS: reactive oxygen species; T-AOC: total antioxidant capacity. Data were shown as mean ± SD ( n ≥ 3). * P < 0.05; ** P < 0.01

Article Snippet: Fasting insulin levels were measured using a mouse insulin ELISA kit (P01325, RayBiotech Life, USA) and the content of chemerin was assayed by the mouse chemerin ELISA kit (EK1330, Boster Biological Technology, China), assays were both according to the manufacturer’s protocols.

Techniques: Fluorescence

Journal: Molecular Medicine

Article Title: Chemerin alleviates the placental oxidative stress and improves fetal overgrowth of gestational diabetes mellitus mice induced by high fat diet

doi: 10.1186/s10020-024-01007-2

Figure Lengend Snippet: The effects of chemerin on the glucose tolerance, weight of fetal mice and placentas, fasting insulin levels, lipid metabolism and liver function. ( A ) The blood glucose of pregnant mice detected by IPGTT on D16.5 (* represents the comparison between the chow groups and the HFD groups; # represents the comparison between the HFD groups and the treated groups). ( B ) The body weight of fetal mice on D18.5. ( C ) The weight of mice placentas on D18.5. ( D ) The sizes of fetal mice and placentas on D18.5 after chemerin treatment. ( E ) The serum level of chemerin on D18.5. ( F ) The serum level of fasting insulin on D18.5. ( G ) The serum level of ALT on D18.5. ( H ) The serum level of AST on D18.5. ( I ) The serum level of HDL on D18.5. ( J ) The serum level of LDL on D18.5. ( K ) The serum level of TC on D18.5. ( L ) The serum level of TG on D18.5. HFD: high fat diet; FINS: fasting insulin; ALT: alanine aminotransferase; AST: aspartate transaminase; HDL: high-density lipoprotein; LDL: low-density lipoprotein; TC: total cholesterol; TG: triglyceride. Data were shown as mean ± SD ( n ≥ 3). # P < 0.05; * P < 0.05; ** P < 0.01; *** P < 0.001

Article Snippet: Fasting insulin levels were measured using a mouse insulin ELISA kit (P01325, RayBiotech Life, USA) and the content of chemerin was assayed by the mouse chemerin ELISA kit (EK1330, Boster Biological Technology, China), assays were both according to the manufacturer’s protocols.

Techniques: Comparison

Journal: Molecular Medicine

Article Title: Chemerin alleviates the placental oxidative stress and improves fetal overgrowth of gestational diabetes mellitus mice induced by high fat diet

doi: 10.1186/s10020-024-01007-2

Figure Lengend Snippet: The localization and expressions of chemerin and DsbA-L in mice placenta of the HFD groups and the chow groups. ( A ) Staining of chemerin and DsbA-L by immunohistochemistry (scale bar = 100 μm). ( B ) Western blotting images of mice placentas. ( C ) Protein quantification of chemerin relative to β-actin. ( D ) AOD of chemerin in mice placenta. ( E ) AOD of DsbA-L in mice placenta. DsbA-L: disulfide-bond A oxidoreductase-like protein; HFD: high fat diet; LA: labyrinth; JZ: junctional zone; AOD: average optical density. Data were shown as mean ± SD ( n ≥ 3). ** P < 0.01, **** P < 0.0001

Article Snippet: Fasting insulin levels were measured using a mouse insulin ELISA kit (P01325, RayBiotech Life, USA) and the content of chemerin was assayed by the mouse chemerin ELISA kit (EK1330, Boster Biological Technology, China), assays were both according to the manufacturer’s protocols.

Techniques: Staining, Immunohistochemistry, Western Blot

Journal: Molecular Medicine

Article Title: Chemerin alleviates the placental oxidative stress and improves fetal overgrowth of gestational diabetes mellitus mice induced by high fat diet

doi: 10.1186/s10020-024-01007-2

Figure Lengend Snippet: The roles and mechanisms of chemerin in the placental oxidative stress and insulin resistance in HFD pregnant mice. ( A ) Western blotting images of mice placentas of three groups. ( B ) Western blotting images of mice placentas between the chow groups and the HFD groups. ( C ) Western blotting images of mice placentas between the HFD groups and the treated groups. The HFD placenta samples presented in ( B ) and ( C ) were from the same several mice. ( D ) Protein quantification of AKT relative to β-actin. ( E ) Protein quantification of IRS1 relative to β-actin. ( F ) Protein quantification of STING relative to β-actin. ( G ) Protein quantification of DsbA-L relative to β-actin. ( H ) The activity of SOD in mice placenta. ( I ) The activity of GSH-Px in mice placenta. ( J ) The activity of CAT in mice placenta. ( K ) The content of MDA in mice placenta. HFD: high fat diet; AKT/PKB: protein kinase B; IRS1: insulin receptor substrate 1; STING: stimulator of interferon genes; DsbA-L: disulfide-bond A oxidoreductase-like protein; SOD: superoxide dismutase; GSH-Px: glutathione peroxidase; CAT: catalase; MDA: malondialdehyde. Data were shown as mean ± SD ( n ≥ 3). * P < 0.05; ** P < 0.01

Article Snippet: Fasting insulin levels were measured using a mouse insulin ELISA kit (P01325, RayBiotech Life, USA) and the content of chemerin was assayed by the mouse chemerin ELISA kit (EK1330, Boster Biological Technology, China), assays were both according to the manufacturer’s protocols.

Techniques: Western Blot, Activity Assay

Journal: Molecular Medicine

Article Title: Chemerin alleviates the placental oxidative stress and improves fetal overgrowth of gestational diabetes mellitus mice induced by high fat diet

doi: 10.1186/s10020-024-01007-2

Figure Lengend Snippet: The long-term impacts of chemerin on blood glucose levels and body weight of maternal and fetal mice. ( A ) The postpartum blood glucose levels of the maternal mice at 8 weeks by IPGTT. ( B ) The blood glucose levels of the mice offspring at 8 weeks old by IPGTT. ( C ) The serum levels of chemerin in the maternal mice at 8 weeks after delivery. ( D ) The serum levels of chemerin in the mice offspring at 8 weeks old. ( E ) The changes of body weight of the mice offspring from 1 to 8 weeks of age. HFD: high fat diet. Data were shown as mean ± SD ( n ≥ 3). * P < 0.05; ** P < 0.01; *** P < 0.001

Article Snippet: Fasting insulin levels were measured using a mouse insulin ELISA kit (P01325, RayBiotech Life, USA) and the content of chemerin was assayed by the mouse chemerin ELISA kit (EK1330, Boster Biological Technology, China), assays were both according to the manufacturer’s protocols.

Techniques:

Journal: Molecular Medicine Reports

Article Title: Oleic acid induces A7r5 cell proliferation and migration associated with increased expression of HGF and p-p38

doi: 10.3892/mmr.2021.12123

Figure Lengend Snippet: Effects of OA on the expression of HGF in A7r5 cells. (A) ELISA results at 24 h identified that low concentrations of OA (50 µmol/l) upregulated the level of HGF, but HGF was downregulated at high concentrations (800 µmol/l) of OA. (B) Western blot analysis at 24 h demonstrated that low concentrations of OA (50 µmol/l) increased the expression of HGF, but high concentrations of OA (800 µmol/l) downregulated this expression. (C) Western blot analysis showed that the increased HGF expression at low concentrations of OA (50 µmol/l) was mitigated by PHA665752 (0.1 µmol/l). (D) Reverse transcription-quantitative PCR analysis at 24 h indicated that the increased expression of HGF at low concentrations of OA (50 µmol/l) was mitigated by PHA665752 (0.1 µmol/l). Data are presented as the mean ± SD (n=3). **P<0.01 and ***P<0.001 vs. 0 µM OA group; ## P<0.01 and ### P<0.001 vs. 50 µmol/l OA group. OA, oleic acid; HGF, hepatocyte growth factor.

Article Snippet: Commercial HGF ELISA kit (cat. no. EK1301) was purchased from Boster Biological Technology.

Techniques: Expressing, Enzyme-linked Immunosorbent Assay, Western Blot, Reverse Transcription, Real-time Polymerase Chain Reaction

Journal: BMC emergency medicine

Article Title: Endothelial glycocalyx degradation is associated with early organ impairment in polytrauma patients.

doi: 10.1186/s12873-021-00446-y

Figure Lengend Snippet: Fig. 1 SDC1 and HS were significantly higher in trauma group compared with control group (69.39 [54.18–130.80] vs. 24.15 [13.89–32.36]; 38.92 [30.47–67.96] vs. 15.55 [11.89–23.24], P < 0.001 respectively)

Article Snippet: Double antibody sandwich ELISA tests were conducted by Enzyme calibration equipment (Thermo scientific Inc., Waltham, MA, USA), to detect SDC1 (EK1339, BOSTER Biological Tech., Wuhan, China), HS (E-EL-H2364c, Elabscience Biotechnology Co., Ltd., Wuhan, China), interleukin-6 (IL-6) (KE00139, Proteintech Group Inc., Rosemont, IL, USA), and tumor necrosis factor-α (TNF-α) (KE00068, Proteintech Group Inc., Rosemont, IL, USA), respectively.

Techniques: Control

Journal: Scientific reports

Article Title: Proteomic analysis of serum extracellular vesicles from biliary tract infection patients to identify novel biomarkers.

doi: 10.1038/s41598-024-56036-y

Figure Lengend Snippet: Figure 5. Validation of CEACAM1 and CRB3 expression in serum EVs. (A) CEACAM1 and CRB3 were detectable by Western blot (WB) analysis (N total = 8). (B) Densitometric quantification of CEACAM1. (C) Densitometric quantification of CRB3. (D) CRB3 was detectable by ELISA analysis. (E) CEACAM1 was detectable by ELISA analysis.* p < 0.05.

Article Snippet: Protein levels were determined using ELISA kits according to the manufacturer’s instructions: Human CEACAM1 ELISA Kit (EK1361, BOSTER, China) and Human Protein Crumbs Homolog 3 (CRB3) ELISA Kit (abx386665, abbexa, UK).

Techniques: Biomarker Discovery, Expressing, Western Blot, Enzyme-linked Immunosorbent Assay