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    Boster Bio mouse il 12 p70 picokine elisa kit
    Secretion of IFN-γ and <t>IL-12</t> from DCs under different stimulations Secretion of IFN-γ A. and IL-12 B. from DCs after incubation with ALA-PDT-treated PECA cells was quantified in cell culture supernatants using <t>ELISA</t> assay, with or without HSP70, HMGB1 or CRT neutralizing antibodies in the co-incubation medium. Unstimulated, immature DCs were used for negative control and DCs incubated with LPS were used for positive control. ALA-PDT-treated PECA cells induced the secretion of IFN-γ and IL-12 markedly higher than that induced by untreated PECA cells ( p
    Mouse Il 12 P70 Picokine Elisa Kit, supplied by Boster Bio, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    mouse il 12 p70 picokine elisa kit - by Bioz Stars, 2022-07
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    Secretion of IFN-γ and IL-12 from DCs under different stimulations Secretion of IFN-γ A. and IL-12 B. from DCs after incubation with ALA-PDT-treated PECA cells was quantified in cell culture supernatants using ELISA assay, with or without HSP70, HMGB1 or CRT neutralizing antibodies in the co-incubation medium. Unstimulated, immature DCs were used for negative control and DCs incubated with LPS were used for positive control. ALA-PDT-treated PECA cells induced the secretion of IFN-γ and IL-12 markedly higher than that induced by untreated PECA cells ( p

    Journal: Oncotarget

    Article Title: Stimulation of dendritic cells by DAMPs in ALA-PDT treated SCC tumor cells

    doi:

    Figure Lengend Snippet: Secretion of IFN-γ and IL-12 from DCs under different stimulations Secretion of IFN-γ A. and IL-12 B. from DCs after incubation with ALA-PDT-treated PECA cells was quantified in cell culture supernatants using ELISA assay, with or without HSP70, HMGB1 or CRT neutralizing antibodies in the co-incubation medium. Unstimulated, immature DCs were used for negative control and DCs incubated with LPS were used for positive control. ALA-PDT-treated PECA cells induced the secretion of IFN-γ and IL-12 markedly higher than that induced by untreated PECA cells ( p

    Article Snippet: In addition, we used mouse HSP70 ELISA Kit (ENEO, USA), mouse HMGB1 ELISA Kit (SHINO-TEST, Japan), mouse IFN-γ and IL-12 ELISA Kit (Boster, China), rabbit anti-Mouse CD80-PE, rabbit anti-Mouse CD86-PE, rabbit anti-Mouse MHC-II-PE, and rat IgG2a K Isotype Control PE (Biolegend, USA).

    Techniques: Incubation, Cell Culture, Enzyme-linked Immunosorbent Assay, Negative Control, Positive Control