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Image Search Results
Journal: Cell Reports Medicine
Article Title: Follicular lymphoma B cells exhibit heterogeneous transcriptional states with associated somatic alterations and tumor microenvironments
doi: 10.1016/j.xcrm.2024.101443
Figure Lengend Snippet: FL B cell states shape their local immune profile (A) Uniform manifold approximation and projection of immune metaclusters from individually profiled FL patient CyTOF samples (n = 60). Clusters identified using Phenograph and are colored by manual annotation. (B) Heatmap of mean surface protein expression in each identified cluster mentioned in (A). Cluster identities listed next to their coordinating colors from (A). (C) Boxplot of immune content as percentage of live cells in each sample by their B cell group. (D) Correlation plot between the difference in CMI vs. INFM state coefficients (positive = higher CMI, negative = higher INFM) and the non-B immune cell percentage (% of live cells). A standard linear regression line is displayed with 95% CIs, along with ( r ) Pearson correlation, and representative p value. (E) (Left) Heatmap of correlation matrix between cell cluster abundances in all of the samples, depicting cellular communities (black boxes). Cell values from Pearson correlation. Dendrogram from correlation distance. (Right) Heatmap of Pearson correlation between cell abundance and sample B cell group state. Pearson p values are printed where p < 0.1. (F) Heatmap of Spearman correlation between single-sample gene set scoring, using SingScore of published GC stromal gene sets, and B cell group states (n = 54). Pearson p values are printed in cells where p < 0.1. (G) CODEX multiplexed immunofluorescence analysis of CD21 (white), CD20 (blue), Ki-67 (yellow), CD4 (green), and CD8 (magenta) on 1 sample from each B cell group. (Left) Baseline cell type abundance in each sample (% of live) from CyTOF. (Right) First 3 panels, stitched images equal to 10× magnification with 500-μm scale. White box delineating the magnified view in panel 4 of follicle and interfollicular region, with 200-μm scale.
Article Snippet:
Techniques: Expressing, Immunofluorescence
Journal: Cell Reports Medicine
Article Title: Follicular lymphoma B cells exhibit heterogeneous transcriptional states with associated somatic alterations and tumor microenvironments
doi: 10.1016/j.xcrm.2024.101443
Figure Lengend Snippet:
Article Snippet:
Techniques: Cell Isolation, Software, Multiplex Assay