Journal: Scientific Reports
Article Title: Transcriptome and proteome analysis of innate immune responses to inactivated Leptospira and bivalent Leptospira vaccines in canine 030-D cells
Figure Lengend Snippet: Expression of proteins associated with innate immune responses in the supernatant of stimulated 030-D cells. 030-D cells were stimulated for 24 h with whole-inactivated L. interrogans serogroup Canicola, Icterohaemorrhagiae, bivalent vaccine B, its excipient, or bivalent vaccine D. The supernatants were assayed for the presence of ( a ) CXCL-10, ( b ) C3, ( c ) IL-1β, ( d ) SAA, ( e ) IL-8 and ( f ) IL-1α. Stimulations were performed in triplicate. Dots represent values of each replicate and bars represent the mean. Three independent experiments were performed with similar outcome. A representative experiment is shown. A one-way ANOVA combined with Dunnett’s multiple comparisons test was used to test for significance. *p
Article Snippet: ELISA kits for canine IL-1α, IL-1β, CXCL-10 and IL-8 (Kingfisher Biotech, Inc) were used in in-house developed multiplex cytokine assays using the Magpix system (Luminex XMAP) according to the manufacturer’s instructions.