Chicken Search Results


90
Bioss anti chicken igg
Generation and coinfection of rTS-H9 and rTS-gB in vitro . (A) Schematic diagram depicting the rNDV cDNA clones rTS-H9 and rTS-gB. (B, C) Viral growth kinetics in BHK-21 (B) and CEF (C) cells. The cells were coinfected or individually infected with rTS-H9/rTS-gB at an MOI of 0.01. Viral titers in culture supernatants collected at 12-hour intervals were determined by IFA on the indicated cell lines. For rTS-H9 + rTS-gB: the proliferation of rTS-H9 was detected using an anti-HA primary antibody. For rTS-gB + rTS-H9: the proliferation of rTS-gB was assessed via an anti-gB primary antibody. (D, E) Foreign protein expression analysis by IFA in BHK-21 (D) and CEF (E) cells. The cells were coinfected or individually infected with rTS-H9 and/or the rTS-gB mixture at an MOI of 0.01. At 48 hpi, the cells were fixed and stained with chicken anti-HA (H9N2) and rabbit anti-gB (ILTV) antibodies, followed <t>by</t> <t>anti-chicken</t> IgG (FITC, green) and rabbit anti-IgG (Cy3, red) antibodies. The nuclei were counterstained with DAPI (blue). Scale bar = 20 μm. (F, G) BHK-21 or CEF cells were coinfected or individually infected with rTS-H9/rTS-gB at an MOI of 0.1. Western blot analysis of BHK-21 (F) and CEF (G) cell lysates harvested at 48 hpi. Proteins were detected using specific antibodies against HA (H9N2 AIV), gB (ILTV), and NDV nucleoprotein (NP). The mouse anti-actin polyclonal antibody served as a loading control.
Anti Chicken Igg, supplied by Bioss, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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92
R&D Systems recombinant chicken
Generation and coinfection of rTS-H9 and rTS-gB in vitro . (A) Schematic diagram depicting the rNDV cDNA clones rTS-H9 and rTS-gB. (B, C) Viral growth kinetics in BHK-21 (B) and CEF (C) cells. The cells were coinfected or individually infected with rTS-H9/rTS-gB at an MOI of 0.01. Viral titers in culture supernatants collected at 12-hour intervals were determined by IFA on the indicated cell lines. For rTS-H9 + rTS-gB: the proliferation of rTS-H9 was detected using an anti-HA primary antibody. For rTS-gB + rTS-H9: the proliferation of rTS-gB was assessed via an anti-gB primary antibody. (D, E) Foreign protein expression analysis by IFA in BHK-21 (D) and CEF (E) cells. The cells were coinfected or individually infected with rTS-H9 and/or the rTS-gB mixture at an MOI of 0.01. At 48 hpi, the cells were fixed and stained with chicken anti-HA (H9N2) and rabbit anti-gB (ILTV) antibodies, followed <t>by</t> <t>anti-chicken</t> IgG (FITC, green) and rabbit anti-IgG (Cy3, red) antibodies. The nuclei were counterstained with DAPI (blue). Scale bar = 20 μm. (F, G) BHK-21 or CEF cells were coinfected or individually infected with rTS-H9/rTS-gB at an MOI of 0.1. Western blot analysis of BHK-21 (F) and CEF (G) cell lysates harvested at 48 hpi. Proteins were detected using specific antibodies against HA (H9N2 AIV), gB (ILTV), and NDV nucleoprotein (NP). The mouse anti-actin polyclonal antibody served as a loading control.
Recombinant Chicken, supplied by R&D Systems, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 92 stars, based on 1 article reviews
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99
R&D Systems myostatin positive sc mstn pax7
Generation and coinfection of rTS-H9 and rTS-gB in vitro . (A) Schematic diagram depicting the rNDV cDNA clones rTS-H9 and rTS-gB. (B, C) Viral growth kinetics in BHK-21 (B) and CEF (C) cells. The cells were coinfected or individually infected with rTS-H9/rTS-gB at an MOI of 0.01. Viral titers in culture supernatants collected at 12-hour intervals were determined by IFA on the indicated cell lines. For rTS-H9 + rTS-gB: the proliferation of rTS-H9 was detected using an anti-HA primary antibody. For rTS-gB + rTS-H9: the proliferation of rTS-gB was assessed via an anti-gB primary antibody. (D, E) Foreign protein expression analysis by IFA in BHK-21 (D) and CEF (E) cells. The cells were coinfected or individually infected with rTS-H9 and/or the rTS-gB mixture at an MOI of 0.01. At 48 hpi, the cells were fixed and stained with chicken anti-HA (H9N2) and rabbit anti-gB (ILTV) antibodies, followed <t>by</t> <t>anti-chicken</t> IgG (FITC, green) and rabbit anti-IgG (Cy3, red) antibodies. The nuclei were counterstained with DAPI (blue). Scale bar = 20 μm. (F, G) BHK-21 or CEF cells were coinfected or individually infected with rTS-H9/rTS-gB at an MOI of 0.1. Western blot analysis of BHK-21 (F) and CEF (G) cell lysates harvested at 48 hpi. Proteins were detected using specific antibodies against HA (H9N2 AIV), gB (ILTV), and NDV nucleoprotein (NP). The mouse anti-actin polyclonal antibody served as a loading control.
Myostatin Positive Sc Mstn Pax7, supplied by R&D Systems, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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92
Novus Biologicals goat anti chicken igg
Generation and coinfection of rTS-H9 and rTS-gB in vitro . (A) Schematic diagram depicting the rNDV cDNA clones rTS-H9 and rTS-gB. (B, C) Viral growth kinetics in BHK-21 (B) and CEF (C) cells. The cells were coinfected or individually infected with rTS-H9/rTS-gB at an MOI of 0.01. Viral titers in culture supernatants collected at 12-hour intervals were determined by IFA on the indicated cell lines. For rTS-H9 + rTS-gB: the proliferation of rTS-H9 was detected using an anti-HA primary antibody. For rTS-gB + rTS-H9: the proliferation of rTS-gB was assessed via an anti-gB primary antibody. (D, E) Foreign protein expression analysis by IFA in BHK-21 (D) and CEF (E) cells. The cells were coinfected or individually infected with rTS-H9 and/or the rTS-gB mixture at an MOI of 0.01. At 48 hpi, the cells were fixed and stained with chicken anti-HA (H9N2) and rabbit anti-gB (ILTV) antibodies, followed <t>by</t> <t>anti-chicken</t> IgG (FITC, green) and rabbit anti-IgG (Cy3, red) antibodies. The nuclei were counterstained with DAPI (blue). Scale bar = 20 μm. (F, G) BHK-21 or CEF cells were coinfected or individually infected with rTS-H9/rTS-gB at an MOI of 0.1. Western blot analysis of BHK-21 (F) and CEF (G) cell lysates harvested at 48 hpi. Proteins were detected using specific antibodies against HA (H9N2 AIV), gB (ILTV), and NDV nucleoprotein (NP). The mouse anti-actin polyclonal antibody served as a loading control.
Goat Anti Chicken Igg, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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99
R&D Systems o4 mab 1326 r d systems
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O4 Mab 1326 R D Systems, supplied by R&D Systems, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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92
R&D Systems igy isotype control
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Igy Isotype Control, supplied by R&D Systems, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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96
Bio-Rad antigen antibody complexes
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Antigen Antibody Complexes, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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94
Jackson Immuno polyclonal dylight 405 affinipure goat anti chicken igy igg
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Polyclonal Dylight 405 Affinipure Goat Anti Chicken Igy Igg, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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96
Jackson Immuno anti chicken 647 jackson immunoresearch 703 605 155 ab 2340379 donkey
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Anti Chicken 647 Jackson Immunoresearch 703 605 155 Ab 2340379 Donkey, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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90
Jackson Immuno chicken serum
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Chicken Serum, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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93
Jackson Immuno aminomethylcoumarin acetate conjugated donkey anti chicken igy
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Aminomethylcoumarin Acetate Conjugated Donkey Anti Chicken Igy, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Jackson Immuno anti chicken alexa 488
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Anti Chicken Alexa 488, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Generation and coinfection of rTS-H9 and rTS-gB in vitro . (A) Schematic diagram depicting the rNDV cDNA clones rTS-H9 and rTS-gB. (B, C) Viral growth kinetics in BHK-21 (B) and CEF (C) cells. The cells were coinfected or individually infected with rTS-H9/rTS-gB at an MOI of 0.01. Viral titers in culture supernatants collected at 12-hour intervals were determined by IFA on the indicated cell lines. For rTS-H9 + rTS-gB: the proliferation of rTS-H9 was detected using an anti-HA primary antibody. For rTS-gB + rTS-H9: the proliferation of rTS-gB was assessed via an anti-gB primary antibody. (D, E) Foreign protein expression analysis by IFA in BHK-21 (D) and CEF (E) cells. The cells were coinfected or individually infected with rTS-H9 and/or the rTS-gB mixture at an MOI of 0.01. At 48 hpi, the cells were fixed and stained with chicken anti-HA (H9N2) and rabbit anti-gB (ILTV) antibodies, followed by anti-chicken IgG (FITC, green) and rabbit anti-IgG (Cy3, red) antibodies. The nuclei were counterstained with DAPI (blue). Scale bar = 20 μm. (F, G) BHK-21 or CEF cells were coinfected or individually infected with rTS-H9/rTS-gB at an MOI of 0.1. Western blot analysis of BHK-21 (F) and CEF (G) cell lysates harvested at 48 hpi. Proteins were detected using specific antibodies against HA (H9N2 AIV), gB (ILTV), and NDV nucleoprotein (NP). The mouse anti-actin polyclonal antibody served as a loading control.

Journal: Poultry Science

Article Title: Co-immunization with two recombinant Newcastle disease viruses expressing ILTV gB and H9N2 AIV HA confers protective efficacy against three avian pathogens

doi: 10.1016/j.psj.2026.106661

Figure Lengend Snippet: Generation and coinfection of rTS-H9 and rTS-gB in vitro . (A) Schematic diagram depicting the rNDV cDNA clones rTS-H9 and rTS-gB. (B, C) Viral growth kinetics in BHK-21 (B) and CEF (C) cells. The cells were coinfected or individually infected with rTS-H9/rTS-gB at an MOI of 0.01. Viral titers in culture supernatants collected at 12-hour intervals were determined by IFA on the indicated cell lines. For rTS-H9 + rTS-gB: the proliferation of rTS-H9 was detected using an anti-HA primary antibody. For rTS-gB + rTS-H9: the proliferation of rTS-gB was assessed via an anti-gB primary antibody. (D, E) Foreign protein expression analysis by IFA in BHK-21 (D) and CEF (E) cells. The cells were coinfected or individually infected with rTS-H9 and/or the rTS-gB mixture at an MOI of 0.01. At 48 hpi, the cells were fixed and stained with chicken anti-HA (H9N2) and rabbit anti-gB (ILTV) antibodies, followed by anti-chicken IgG (FITC, green) and rabbit anti-IgG (Cy3, red) antibodies. The nuclei were counterstained with DAPI (blue). Scale bar = 20 μm. (F, G) BHK-21 or CEF cells were coinfected or individually infected with rTS-H9/rTS-gB at an MOI of 0.1. Western blot analysis of BHK-21 (F) and CEF (G) cell lysates harvested at 48 hpi. Proteins were detected using specific antibodies against HA (H9N2 AIV), gB (ILTV), and NDV nucleoprotein (NP). The mouse anti-actin polyclonal antibody served as a loading control.

Article Snippet: Subsequently, fluorescein isothiocyanate (FITC)-conjugated anti-chicken IgG (1:200. bs0310R-FITC, Bioss) and Cy3-labeled anti-rabbit IgG (1:500.

Techniques: In Vitro, Clone Assay, Infection, Expressing, Staining, Western Blot, Control

KEY RESOURCES TABLE

Journal: Cell reports

Article Title: Variation of Human Neural Stem Cells Generating Organizer States In Vitro before Committing to Cortical Excitatory or Inhibitory Neuronal Fates

doi: 10.1016/j.celrep.2020.107599

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: The following primary antibodies were used at the concentration indicated by manufacturer’s protocol: CaM Kinase II alpha (6G9) (NB100–1983), LMX1A (NBP1–81303) Novusbio; SYNAPSIN (106 001), HOMER (160 003) Synaptic System; EGFR (Ab231), FGFR1 phosphoY654 (Ab59194), TBR1 (Ab31940), REELIN (Ab18570), CYCLIN D1 (Ab10540), FGFR2 (Ab10648), BMPR1A (Ab38560) Abcam; HES1 (11988), p-SMAD1/5 (9516), CYCLIN D1 (2926), pERK1/2 (4370), FGFR1 (9740) Cell Signaling Technology; PAX6 (PRB-278P) BioLegend; NESTIN (MAB1259), OTX2 (AF1979), PDGFR alpha (AF1062; AF307), SOX2 (AF2018; MAB2018), SOX21 (AF3538), TuJ1 (MAB1195), EGFR (AF1280), O4 (MAB 1326) R&D Systems; GFAP (Z 0334) DAKO; HES5 (sc-13859), CUX1 (sc-13024), TLE4 (sc-9125), FGFR3 (sc-9007), LHX2 (sc-19344) Santa Cruz Biotechnology; FOXP2 (ABE73), TBR1 (AB2261), REELIN (MAB5366) Millipore; FOXG1 (M227) Takara/Clontech; anti GAD65/67 was kindly gifted by Dr. Christian Geis, Hans Berger Department of Neurology, Jena University Hospital, Germany ( ).

Techniques: Virus, Plasmid Preparation, Recombinant, Transfection, Antibody Labeling, In Vitro, Microarray, Gene Expression, Derivative Assay, Software