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  • 99
    Millipore cyclohexamide
    Cyclohexamide, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    86
    Novoprotein cycloheximide chx
    Mitotic slippage is induced in <t>nocodazole-treated</t> cells after inhibition of transcription or translation. Characterization of NIH3T3 cells: control cells, nocodazole (noc)-treated cells (arrested in mitosis), noc-cells incubated with cycloheximide <t>(CHX;</t> 35.5 µM) or actinomycin D (ActD; 8 µM) for 4 h. A) Phase-contrast microscopy; magnification: 200×. B) Immunofluorescence microscopy; arrowheads indicate mitotic cells and arrows indicate cells that underwent mitotic slippage; DAPI stains the DNA (blue) and anti-α-tubulin antibody stains the microtubules (green). Scale bar = 25 µm. C) Mitotic and multinucleation indexes of noc-treated cells with or without CHX or ActD for 4 h. Data presented as mean ± S.D. from three independent experiments; ** p
    Cycloheximide Chx, supplied by Novoprotein, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    86
    Novoprotein chx
    Mitotic slippage is induced in <t>nocodazole-treated</t> cells after inhibition of transcription or translation. Characterization of NIH3T3 cells: control cells, nocodazole (noc)-treated cells (arrested in mitosis), noc-cells incubated with cycloheximide <t>(CHX;</t> 35.5 µM) or actinomycin D (ActD; 8 µM) for 4 h. A) Phase-contrast microscopy; magnification: 200×. B) Immunofluorescence microscopy; arrowheads indicate mitotic cells and arrows indicate cells that underwent mitotic slippage; DAPI stains the DNA (blue) and anti-α-tubulin antibody stains the microtubules (green). Scale bar = 25 µm. C) Mitotic and multinucleation indexes of noc-treated cells with or without CHX or ActD for 4 h. Data presented as mean ± S.D. from three independent experiments; ** p
    Chx, supplied by Novoprotein, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Mitotic slippage is induced in nocodazole-treated cells after inhibition of transcription or translation. Characterization of NIH3T3 cells: control cells, nocodazole (noc)-treated cells (arrested in mitosis), noc-cells incubated with cycloheximide (CHX; 35.5 µM) or actinomycin D (ActD; 8 µM) for 4 h. A) Phase-contrast microscopy; magnification: 200×. B) Immunofluorescence microscopy; arrowheads indicate mitotic cells and arrows indicate cells that underwent mitotic slippage; DAPI stains the DNA (blue) and anti-α-tubulin antibody stains the microtubules (green). Scale bar = 25 µm. C) Mitotic and multinucleation indexes of noc-treated cells with or without CHX or ActD for 4 h. Data presented as mean ± S.D. from three independent experiments; ** p

    Journal: PLoS ONE

    Article Title: Sustained Spindle-Assembly Checkpoint Response Requires De Novo Transcription and Translation of Cyclin B1

    doi: 10.1371/journal.pone.0013037

    Figure Lengend Snippet: Mitotic slippage is induced in nocodazole-treated cells after inhibition of transcription or translation. Characterization of NIH3T3 cells: control cells, nocodazole (noc)-treated cells (arrested in mitosis), noc-cells incubated with cycloheximide (CHX; 35.5 µM) or actinomycin D (ActD; 8 µM) for 4 h. A) Phase-contrast microscopy; magnification: 200×. B) Immunofluorescence microscopy; arrowheads indicate mitotic cells and arrows indicate cells that underwent mitotic slippage; DAPI stains the DNA (blue) and anti-α-tubulin antibody stains the microtubules (green). Scale bar = 25 µm. C) Mitotic and multinucleation indexes of noc-treated cells with or without CHX or ActD for 4 h. Data presented as mean ± S.D. from three independent experiments; ** p

    Article Snippet: NIH3T3 cells were incubated for 14 h with nocodazole and further treated for 4 h with either 35.5 µM of cycloheximide (CHX), a de novo protein synthesis inhibitor , or 8 µM of actinomycin D (ActD), an inhibitor of transcription .

    Techniques: Inhibition, Incubation, Microscopy, Immunofluorescence

    Overexpression of Cyclin B1 protein rescues the mitotic phenotype. HEK293 cells were transfected with the wild-type and the non-degradable forms of Cyclin B1 (CyclinB1-wt and CyclinB1-R42A, respectively); pCMS-EGFP was used as transfection control. A, B and C) Analysis of Cyclin B1 endogenous (indicated with one asterisk) and exogenous (indicated with double asterisk) protein levels by western blotting in cells transfected with pCMS-EGFP (A), CyclinB1-wt (B) and CyclinB1-R42A (C). After transfection, HEK293 cells were incubated with nocodazole (noc) for 14 h and treated with actinomycin D (ActD; 8 µM) or cycloheximide (CHX; 35.5 µM) for 6 h; β-actin was used as loading control. All lanes presented in C are from the same experiment. D) Mitotic index of nocodazole (Noc) treated cells with or without CHX or ActD for 6 h. Data presented as mean ± S.D. from three independent experiments; *** p

    Journal: PLoS ONE

    Article Title: Sustained Spindle-Assembly Checkpoint Response Requires De Novo Transcription and Translation of Cyclin B1

    doi: 10.1371/journal.pone.0013037

    Figure Lengend Snippet: Overexpression of Cyclin B1 protein rescues the mitotic phenotype. HEK293 cells were transfected with the wild-type and the non-degradable forms of Cyclin B1 (CyclinB1-wt and CyclinB1-R42A, respectively); pCMS-EGFP was used as transfection control. A, B and C) Analysis of Cyclin B1 endogenous (indicated with one asterisk) and exogenous (indicated with double asterisk) protein levels by western blotting in cells transfected with pCMS-EGFP (A), CyclinB1-wt (B) and CyclinB1-R42A (C). After transfection, HEK293 cells were incubated with nocodazole (noc) for 14 h and treated with actinomycin D (ActD; 8 µM) or cycloheximide (CHX; 35.5 µM) for 6 h; β-actin was used as loading control. All lanes presented in C are from the same experiment. D) Mitotic index of nocodazole (Noc) treated cells with or without CHX or ActD for 6 h. Data presented as mean ± S.D. from three independent experiments; *** p

    Article Snippet: NIH3T3 cells were incubated for 14 h with nocodazole and further treated for 4 h with either 35.5 µM of cycloheximide (CHX), a de novo protein synthesis inhibitor , or 8 µM of actinomycin D (ActD), an inhibitor of transcription .

    Techniques: Over Expression, Transfection, Western Blot, Incubation

    Cyclin B1 and Securin are degraded during mitotic slippage induced by inhibition of transcription and translation. A) Analysis of Cyclin B1, Securin and CDK1 levels by western blotting. NIH3T3 cells were incubated with nocodazole (noc) for 14 h and treated with actinomycin D (ActD; 8 µM) or cycloheximide (CHX; 35.5 µM) with or without the inhibitor of the proteasome MG132 (25 µM), for 4 h; β-actin was used as loading control. B) Quantification of Cyclin B1 and Securin proteins in noc-, noc-ActD-, noc-ActD-MG, noc-CHX and noc-CHX-MG-cells. Normalization of Cyclin B1 and Securin was calculated on the ratio of these proteins per β-actin protein present in each condition. Data presented as mean ± S.D. from three independent experiments. C and D) Immunofluorescence microscopy: asynchronous population (control) and noc-cells treated with ActD for 4 h; DAPI stains the DNA (blue) and Cyclin B1 (C) and Securin (D) are stained in green. Arrowheads point mitotic cells and arrows point cell that undergone mitotic slippage. The adapted cells shown are representative of both ActD and CHX induced mitotic slippage. Scale bar = 25 µm.

    Journal: PLoS ONE

    Article Title: Sustained Spindle-Assembly Checkpoint Response Requires De Novo Transcription and Translation of Cyclin B1

    doi: 10.1371/journal.pone.0013037

    Figure Lengend Snippet: Cyclin B1 and Securin are degraded during mitotic slippage induced by inhibition of transcription and translation. A) Analysis of Cyclin B1, Securin and CDK1 levels by western blotting. NIH3T3 cells were incubated with nocodazole (noc) for 14 h and treated with actinomycin D (ActD; 8 µM) or cycloheximide (CHX; 35.5 µM) with or without the inhibitor of the proteasome MG132 (25 µM), for 4 h; β-actin was used as loading control. B) Quantification of Cyclin B1 and Securin proteins in noc-, noc-ActD-, noc-ActD-MG, noc-CHX and noc-CHX-MG-cells. Normalization of Cyclin B1 and Securin was calculated on the ratio of these proteins per β-actin protein present in each condition. Data presented as mean ± S.D. from three independent experiments. C and D) Immunofluorescence microscopy: asynchronous population (control) and noc-cells treated with ActD for 4 h; DAPI stains the DNA (blue) and Cyclin B1 (C) and Securin (D) are stained in green. Arrowheads point mitotic cells and arrows point cell that undergone mitotic slippage. The adapted cells shown are representative of both ActD and CHX induced mitotic slippage. Scale bar = 25 µm.

    Article Snippet: NIH3T3 cells were incubated for 14 h with nocodazole and further treated for 4 h with either 35.5 µM of cycloheximide (CHX), a de novo protein synthesis inhibitor , or 8 µM of actinomycin D (ActD), an inhibitor of transcription .

    Techniques: Inhibition, Western Blot, Incubation, Immunofluorescence, Microscopy, Staining

    Inhibition of transcription or translation induces mitotic slippage in nocodazole treated HEK293 cells. Characterization of HEK293 cells: control cells, nocodazole (noc)-treated cells (arrested in mitosis), noc-cells incubated with cycloheximide (CHX; 35.5 µM) or actinomycin D (ActD; 8 µM) for 6 h. A) Phase-contrast microscopy; magnification: 200×. B) Mitotic index of noc-treated cells with or without CHX or ActD for 6 h. Data presented as mean ± S.D. from three independent experiments; ** p

    Journal: PLoS ONE

    Article Title: Sustained Spindle-Assembly Checkpoint Response Requires De Novo Transcription and Translation of Cyclin B1

    doi: 10.1371/journal.pone.0013037

    Figure Lengend Snippet: Inhibition of transcription or translation induces mitotic slippage in nocodazole treated HEK293 cells. Characterization of HEK293 cells: control cells, nocodazole (noc)-treated cells (arrested in mitosis), noc-cells incubated with cycloheximide (CHX; 35.5 µM) or actinomycin D (ActD; 8 µM) for 6 h. A) Phase-contrast microscopy; magnification: 200×. B) Mitotic index of noc-treated cells with or without CHX or ActD for 6 h. Data presented as mean ± S.D. from three independent experiments; ** p

    Article Snippet: NIH3T3 cells were incubated for 14 h with nocodazole and further treated for 4 h with either 35.5 µM of cycloheximide (CHX), a de novo protein synthesis inhibitor , or 8 µM of actinomycin D (ActD), an inhibitor of transcription .

    Techniques: Inhibition, Incubation, Microscopy