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    • cufi 1 cell line  (ATCC)
    93
    ATCC cufi 1 cell line
    ER Stress and UPR activation in CF HBECs. (A) mRNA relative expression of ER stress and UPR markers BiP, IRE1α, ATF6, PERK, XBP1s, XBP1u, ATF4, CHOP, GADD34, and IL-6 in BEAS-2B, <t>CuFi-1,</t> and CuFi-4 cell lines. (B–D) Single cells were gated and used to measure the mean fluorescent intensity of each cell line though flow cytometry; IRE1α (B) and phosphorylated IRE1α (C) protein expression was measured with monoclonal conjugated antibodies (PE, PerCP) in BEAS-2B, CuFi-1, and CuFi-4 cell lines (D) . All antibodies were normalized with their respective isotype controls, in each cell line. (E–G) XBP1s and IL-6 were measured in response to LPS (100 ng/ml) and Tg (300 nM) for 4 h measuring XBP1s mRNA (E), IL-6 mRNA (F) and IL-6 cytokine levels (G) ; when referred, the IRE1α inhibitor, 4 μ8c (50 μM), was used 30 min before each stimulation. All data is presented as mean ± SEM and mRNA data represented by logarithmic scale base 10. Statistical significance was determined using 2way-ANOVA, Dunnett's test (A) , unpaired (D) or paired (E–G) independent student's t -test. * p < 0.05, ** p < 0.01, *** p < 0.001. n = 4 biological replicates for all cell lines.
    Cufi 1 Cell Line, supplied by ATCC, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    ER Stress and UPR activation in CF HBECs. (A) mRNA relative expression of ER stress and UPR markers BiP, IRE1α, ATF6, PERK, XBP1s, XBP1u, ATF4, CHOP, GADD34, and IL-6 in BEAS-2B, CuFi-1, and CuFi-4 cell lines. (B–D) Single cells were gated and used to measure the mean fluorescent intensity of each cell line though flow cytometry; IRE1α (B) and phosphorylated IRE1α (C) protein expression was measured with monoclonal conjugated antibodies (PE, PerCP) in BEAS-2B, CuFi-1, and CuFi-4 cell lines (D) . All antibodies were normalized with their respective isotype controls, in each cell line. (E–G) XBP1s and IL-6 were measured in response to LPS (100 ng/ml) and Tg (300 nM) for 4 h measuring XBP1s mRNA (E), IL-6 mRNA (F) and IL-6 cytokine levels (G) ; when referred, the IRE1α inhibitor, 4 μ8c (50 μM), was used 30 min before each stimulation. All data is presented as mean ± SEM and mRNA data represented by logarithmic scale base 10. Statistical significance was determined using 2way-ANOVA, Dunnett's test (A) , unpaired (D) or paired (E–G) independent student's t -test. * p < 0.05, ** p < 0.01, *** p < 0.001. n = 4 biological replicates for all cell lines.

    Journal: Frontiers in Immunology

    Article Title: Metabolic Reprograming of Cystic Fibrosis Macrophages via the IRE1α Arm of the Unfolded Protein Response Results in Exacerbated Inflammation

    doi: 10.3389/fimmu.2019.01789

    Figure Lengend Snippet: ER Stress and UPR activation in CF HBECs. (A) mRNA relative expression of ER stress and UPR markers BiP, IRE1α, ATF6, PERK, XBP1s, XBP1u, ATF4, CHOP, GADD34, and IL-6 in BEAS-2B, CuFi-1, and CuFi-4 cell lines. (B–D) Single cells were gated and used to measure the mean fluorescent intensity of each cell line though flow cytometry; IRE1α (B) and phosphorylated IRE1α (C) protein expression was measured with monoclonal conjugated antibodies (PE, PerCP) in BEAS-2B, CuFi-1, and CuFi-4 cell lines (D) . All antibodies were normalized with their respective isotype controls, in each cell line. (E–G) XBP1s and IL-6 were measured in response to LPS (100 ng/ml) and Tg (300 nM) for 4 h measuring XBP1s mRNA (E), IL-6 mRNA (F) and IL-6 cytokine levels (G) ; when referred, the IRE1α inhibitor, 4 μ8c (50 μM), was used 30 min before each stimulation. All data is presented as mean ± SEM and mRNA data represented by logarithmic scale base 10. Statistical significance was determined using 2way-ANOVA, Dunnett's test (A) , unpaired (D) or paired (E–G) independent student's t -test. * p < 0.05, ** p < 0.01, *** p < 0.001. n = 4 biological replicates for all cell lines.

    Article Snippet: CuFi-1 cell line , ATCC , ATCC CRL-4013.

    Techniques: Activation Assay, Expressing, Flow Cytometry

    Table of Reagents

    Journal: Frontiers in Immunology

    Article Title: Metabolic Reprograming of Cystic Fibrosis Macrophages via the IRE1α Arm of the Unfolded Protein Response Results in Exacerbated Inflammation

    doi: 10.3389/fimmu.2019.01789

    Figure Lengend Snippet: Table of Reagents

    Article Snippet: CuFi-1 cell line , ATCC , ATCC CRL-4013.

    Techniques: Purification, Recombinant, Isolation, SYBR Green Assay, Protease Inhibitor, Western Blot, Enzyme-linked Immunosorbent Assay, Conjugation Assay, Bicinchoninic Acid Protein Assay, Software