CRL-1596 Search Results


ramos  (ATCC)
99
ATCC ramos
Expression of hsa-miR-127 after transient transfection with EBNA1 in <t>Ramos</t> and human memory B cells. ( a ) The percentage of cells expressing GFP was determined by FACS analysis in Ramos cells 48 h post transfection (Ramos+pmax GFP ) and compared with the control (Ramos mock). A transfection efficiency of 35% was achieved. ( b ) EBNA1 protein levels were evaluated by WB in the EBV-negative BL cell line (Ramos) after transient transfection of EBNA1 . EBNA1 was upregulated 48 h after transfection. Lane 1—Ramos mock; lane 2—Ramos transfected with the empty vector pcDNA3; lane 3—Ramos transfected with pcDNA3- EBNA1 ; lane 4—positive <t>control</t> <t>(Raji).</t> ( c ) Expression of hsa-miR-127 was evaluated by qRT-PCR in the EBV-negative BL cell line (Ramos) after transient transfection of EBNA1 . Hsa-miR-127 was upregulated 48 h after transfection in Ramos cells transfected with EBNA1 , in comparison with the controls (Ramos mock and Ramos+ empty vector). The graph shows the results of three independent experiments. Error bars represent s.d. between replicates. ( d ) The percentage of cells expressing GFP was determined by FACS analysis in memory B cells 24 h post transfection (memory B cells+pmax GFP ) and compared with the control (memory B cells mock). A transfection efficiency of 33% was achieved. ( e ) EBNA1 protein levels in memory B cells were evaluated by indirect immunofluorescence after transient transfection of EBNA1 . EBNA1 was upregulated 24 h after transfection. ( f ) Expression of hsa-miR-127 was evaluated by qRT-PCR in human memory B cells purified from the peripheral blood of normal donors after transient transfection of EBNA1 . Hsa-miR-127 was upregulated 24 h after transfection in memory B cells transfected with EBNA1 , in comparison with the controls (memory B cells mock and memory B cells+ empty vector). The graph shows the results of three independent experiments. Error bars represent s.d. between replicates.
Ramos, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/ramos/product/ATCC
Average 99 stars, based on 1 article reviews
ramos - by Bioz Stars, 2026-02
99/100 stars
  Buy from Supplier
crl-1596  (LGC Standards)
97
LGC Standards crl-1596
Expression of hsa-miR-127 after transient transfection with EBNA1 in <t>Ramos</t> and human memory B cells. ( a ) The percentage of cells expressing GFP was determined by FACS analysis in Ramos cells 48 h post transfection (Ramos+pmax GFP ) and compared with the control (Ramos mock). A transfection efficiency of 35% was achieved. ( b ) EBNA1 protein levels were evaluated by WB in the EBV-negative BL cell line (Ramos) after transient transfection of EBNA1 . EBNA1 was upregulated 48 h after transfection. Lane 1—Ramos mock; lane 2—Ramos transfected with the empty vector pcDNA3; lane 3—Ramos transfected with pcDNA3- EBNA1 ; lane 4—positive <t>control</t> <t>(Raji).</t> ( c ) Expression of hsa-miR-127 was evaluated by qRT-PCR in the EBV-negative BL cell line (Ramos) after transient transfection of EBNA1 . Hsa-miR-127 was upregulated 48 h after transfection in Ramos cells transfected with EBNA1 , in comparison with the controls (Ramos mock and Ramos+ empty vector). The graph shows the results of three independent experiments. Error bars represent s.d. between replicates. ( d ) The percentage of cells expressing GFP was determined by FACS analysis in memory B cells 24 h post transfection (memory B cells+pmax GFP ) and compared with the control (memory B cells mock). A transfection efficiency of 33% was achieved. ( e ) EBNA1 protein levels in memory B cells were evaluated by indirect immunofluorescence after transient transfection of EBNA1 . EBNA1 was upregulated 24 h after transfection. ( f ) Expression of hsa-miR-127 was evaluated by qRT-PCR in human memory B cells purified from the peripheral blood of normal donors after transient transfection of EBNA1 . Hsa-miR-127 was upregulated 24 h after transfection in memory B cells transfected with EBNA1 , in comparison with the controls (memory B cells mock and memory B cells+ empty vector). The graph shows the results of three independent experiments. Error bars represent s.d. between replicates.
Crl 1596, supplied by LGC Standards, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/crl-1596/product/LGC Standards
Average 97 stars, based on 1 article reviews
crl-1596 - by Bioz Stars, 2026-02
97/100 stars
  Buy from Supplier

Image Search Results


Expression of hsa-miR-127 after transient transfection with EBNA1 in Ramos and human memory B cells. ( a ) The percentage of cells expressing GFP was determined by FACS analysis in Ramos cells 48 h post transfection (Ramos+pmax GFP ) and compared with the control (Ramos mock). A transfection efficiency of 35% was achieved. ( b ) EBNA1 protein levels were evaluated by WB in the EBV-negative BL cell line (Ramos) after transient transfection of EBNA1 . EBNA1 was upregulated 48 h after transfection. Lane 1—Ramos mock; lane 2—Ramos transfected with the empty vector pcDNA3; lane 3—Ramos transfected with pcDNA3- EBNA1 ; lane 4—positive control (Raji). ( c ) Expression of hsa-miR-127 was evaluated by qRT-PCR in the EBV-negative BL cell line (Ramos) after transient transfection of EBNA1 . Hsa-miR-127 was upregulated 48 h after transfection in Ramos cells transfected with EBNA1 , in comparison with the controls (Ramos mock and Ramos+ empty vector). The graph shows the results of three independent experiments. Error bars represent s.d. between replicates. ( d ) The percentage of cells expressing GFP was determined by FACS analysis in memory B cells 24 h post transfection (memory B cells+pmax GFP ) and compared with the control (memory B cells mock). A transfection efficiency of 33% was achieved. ( e ) EBNA1 protein levels in memory B cells were evaluated by indirect immunofluorescence after transient transfection of EBNA1 . EBNA1 was upregulated 24 h after transfection. ( f ) Expression of hsa-miR-127 was evaluated by qRT-PCR in human memory B cells purified from the peripheral blood of normal donors after transient transfection of EBNA1 . Hsa-miR-127 was upregulated 24 h after transfection in memory B cells transfected with EBNA1 , in comparison with the controls (memory B cells mock and memory B cells+ empty vector). The graph shows the results of three independent experiments. Error bars represent s.d. between replicates.

Journal: Blood Cancer Journal

Article Title: Epstein-Barr nuclear antigen 1 induces expression of the cellular microRNA hsa-miR-127 and impairing B-cell differentiation in EBV-infected memory B cells. New insights into the pathogenesis of Burkitt lymphoma

doi: 10.1038/bcj.2012.29

Figure Lengend Snippet: Expression of hsa-miR-127 after transient transfection with EBNA1 in Ramos and human memory B cells. ( a ) The percentage of cells expressing GFP was determined by FACS analysis in Ramos cells 48 h post transfection (Ramos+pmax GFP ) and compared with the control (Ramos mock). A transfection efficiency of 35% was achieved. ( b ) EBNA1 protein levels were evaluated by WB in the EBV-negative BL cell line (Ramos) after transient transfection of EBNA1 . EBNA1 was upregulated 48 h after transfection. Lane 1—Ramos mock; lane 2—Ramos transfected with the empty vector pcDNA3; lane 3—Ramos transfected with pcDNA3- EBNA1 ; lane 4—positive control (Raji). ( c ) Expression of hsa-miR-127 was evaluated by qRT-PCR in the EBV-negative BL cell line (Ramos) after transient transfection of EBNA1 . Hsa-miR-127 was upregulated 48 h after transfection in Ramos cells transfected with EBNA1 , in comparison with the controls (Ramos mock and Ramos+ empty vector). The graph shows the results of three independent experiments. Error bars represent s.d. between replicates. ( d ) The percentage of cells expressing GFP was determined by FACS analysis in memory B cells 24 h post transfection (memory B cells+pmax GFP ) and compared with the control (memory B cells mock). A transfection efficiency of 33% was achieved. ( e ) EBNA1 protein levels in memory B cells were evaluated by indirect immunofluorescence after transient transfection of EBNA1 . EBNA1 was upregulated 24 h after transfection. ( f ) Expression of hsa-miR-127 was evaluated by qRT-PCR in human memory B cells purified from the peripheral blood of normal donors after transient transfection of EBNA1 . Hsa-miR-127 was upregulated 24 h after transfection in memory B cells transfected with EBNA1 , in comparison with the controls (memory B cells mock and memory B cells+ empty vector). The graph shows the results of three independent experiments. Error bars represent s.d. between replicates.

Article Snippet: Raji (EBV-positive BL-derived cell line), Ramos (EBV-negative BL-derived cell line), 293T (human embryonic kidney cell line) and HeLa (cervical cancer cell line) cells were obtained from the American Type Culture Collection (ATCC, Milan, Italy) (references: #CCL-86, #CRL-1596, #CCL-2, #CRL-11268).

Techniques: Expressing, Transfection, Control, Plasmid Preparation, Positive Control, Quantitative RT-PCR, Comparison, Immunofluorescence, Purification