CRL-1424 Search Results


malignant melanoma cell line g361  (ATCC)
96
ATCC malignant melanoma cell line g361
Cytotoxic effects of CA on <t>G361</t> cells. ( A ) Chemical structure of caffeic acid. ( B ) G361 and SK-MEL-24 cells were treated with various concentrations of CA (0, 80, 160, 240, and 320 μM) for 24, 48, and 72 h. Cell viability was measured using the MTT assay. IC 50 values for G361 cells were calculated for each time point. * p < 0.05 versus control. ( C ) Intracellular ATP levels were measured after treatment with CA (0, 20, 40, and 80 μM) for 24 h. ( D ) Phase contrast microscopy images of G361 cells treated with CA (0, 20, 40, and 80 μM) for 24 h. Scale bar: 50 μm. ( E ) Nuclear morphology was assessed using DAPI staining after 24 h of CA treatment. Apoptotic nuclear condensation and fragmentation were observed in a dose-dependent manner. Scale bar: 25 μm. ( F ) Quantification of apoptotic cells using the Annexin V-PE binding assay and Muse Cell Analyzer after 24 h of CA treatment.
Malignant Melanoma Cell Line G361, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/malignant melanoma cell line g361/product/ATCC
Average 96 stars, based on 1 article reviews
malignant melanoma cell line g361 - by Bioz Stars, 2026-02
96/100 stars
  Buy from Supplier
crl-1424  (LGC Standards)
96
LGC Standards crl-1424
Cytotoxic effects of CA on <t>G361</t> cells. ( A ) Chemical structure of caffeic acid. ( B ) G361 and SK-MEL-24 cells were treated with various concentrations of CA (0, 80, 160, 240, and 320 μM) for 24, 48, and 72 h. Cell viability was measured using the MTT assay. IC 50 values for G361 cells were calculated for each time point. * p < 0.05 versus control. ( C ) Intracellular ATP levels were measured after treatment with CA (0, 20, 40, and 80 μM) for 24 h. ( D ) Phase contrast microscopy images of G361 cells treated with CA (0, 20, 40, and 80 μM) for 24 h. Scale bar: 50 μm. ( E ) Nuclear morphology was assessed using DAPI staining after 24 h of CA treatment. Apoptotic nuclear condensation and fragmentation were observed in a dose-dependent manner. Scale bar: 25 μm. ( F ) Quantification of apoptotic cells using the Annexin V-PE binding assay and Muse Cell Analyzer after 24 h of CA treatment.
Crl 1424, supplied by LGC Standards, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/crl-1424/product/LGC Standards
Average 96 stars, based on 1 article reviews
crl-1424 - by Bioz Stars, 2026-02
96/100 stars
  Buy from Supplier

Image Search Results


Cytotoxic effects of CA on G361 cells. ( A ) Chemical structure of caffeic acid. ( B ) G361 and SK-MEL-24 cells were treated with various concentrations of CA (0, 80, 160, 240, and 320 μM) for 24, 48, and 72 h. Cell viability was measured using the MTT assay. IC 50 values for G361 cells were calculated for each time point. * p < 0.05 versus control. ( C ) Intracellular ATP levels were measured after treatment with CA (0, 20, 40, and 80 μM) for 24 h. ( D ) Phase contrast microscopy images of G361 cells treated with CA (0, 20, 40, and 80 μM) for 24 h. Scale bar: 50 μm. ( E ) Nuclear morphology was assessed using DAPI staining after 24 h of CA treatment. Apoptotic nuclear condensation and fragmentation were observed in a dose-dependent manner. Scale bar: 25 μm. ( F ) Quantification of apoptotic cells using the Annexin V-PE binding assay and Muse Cell Analyzer after 24 h of CA treatment.

Journal: Biomedicines

Article Title: Apoptosis, Cell Growth, and Glycogen Synthase Kinase 3β Phosphorylation in Caffeic Acid-Treated Human Malignant Melanoma Cells

doi: 10.3390/biomedicines13102389

Figure Lengend Snippet: Cytotoxic effects of CA on G361 cells. ( A ) Chemical structure of caffeic acid. ( B ) G361 and SK-MEL-24 cells were treated with various concentrations of CA (0, 80, 160, 240, and 320 μM) for 24, 48, and 72 h. Cell viability was measured using the MTT assay. IC 50 values for G361 cells were calculated for each time point. * p < 0.05 versus control. ( C ) Intracellular ATP levels were measured after treatment with CA (0, 20, 40, and 80 μM) for 24 h. ( D ) Phase contrast microscopy images of G361 cells treated with CA (0, 20, 40, and 80 μM) for 24 h. Scale bar: 50 μm. ( E ) Nuclear morphology was assessed using DAPI staining after 24 h of CA treatment. Apoptotic nuclear condensation and fragmentation were observed in a dose-dependent manner. Scale bar: 25 μm. ( F ) Quantification of apoptotic cells using the Annexin V-PE binding assay and Muse Cell Analyzer after 24 h of CA treatment.

Article Snippet: The human malignant melanoma cell line G361 and SK-MEL-24 was obtained from the American Type Culture Collection (Manassas, VA, USA).

Techniques: MTT Assay, Control, Microscopy, Staining, Binding Assay