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  • 86
    Roche atp bioluminescence assay kit cls ii
    Os affects mitochondrial function and morphology. ( a ) HCT116 cells were treated with Os (50 μ M) for 16 h, fixed and viewed under electron microscope (magnification × 25 000) White arrows pointing to mitochondria. ( b ) Relative <t>ATP</t> level was determined in HCT116 cells using the ATP Bioluminescence Assay Kit <t>CLS</t> II (Roche), as described in Materials and Methods. ( c ) A total of 6 × 10 5 HCT116 cells were incubated with 50 μ M of Os for 4 h and ETC-II activity was determined using the Human Complex activity Microplate Assay Kit (Mitosciences). ( d ) Os-treated cells were incubated with the potential-sensitive probe DiOC 6 , and ΔΨ m was analyzed by flow cytometry as described in Materials and Methods. Representative bar graphs were plotted with the G-mean values to determine the fold difference in ΔΨ m between control and Os-treated groups. ( e ) Os-treated cells were loaded with N-nonyl-acridine-orange, and cardiolipin oxidation was analyzed by flow cytometry as described in Materials and Methods. Quantification of G-mean values have been reported on the corresponding graphs. • P
    Atp Bioluminescence Assay Kit Cls Ii, supplied by Roche, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/atp bioluminescence assay kit cls ii/product/Roche
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    atp bioluminescence assay kit cls ii - by Bioz Stars, 2021-05
    86/100 stars
      Buy from Supplier

    97
    PerkinElmer operetta high content imaging system
    Os affects mitochondrial function and morphology. ( a ) HCT116 cells were treated with Os (50 μ M) for 16 h, fixed and viewed under electron microscope (magnification × 25 000) White arrows pointing to mitochondria. ( b ) Relative <t>ATP</t> level was determined in HCT116 cells using the ATP Bioluminescence Assay Kit <t>CLS</t> II (Roche), as described in Materials and Methods. ( c ) A total of 6 × 10 5 HCT116 cells were incubated with 50 μ M of Os for 4 h and ETC-II activity was determined using the Human Complex activity Microplate Assay Kit (Mitosciences). ( d ) Os-treated cells were incubated with the potential-sensitive probe DiOC 6 , and ΔΨ m was analyzed by flow cytometry as described in Materials and Methods. Representative bar graphs were plotted with the G-mean values to determine the fold difference in ΔΨ m between control and Os-treated groups. ( e ) Os-treated cells were loaded with N-nonyl-acridine-orange, and cardiolipin oxidation was analyzed by flow cytometry as described in Materials and Methods. Quantification of G-mean values have been reported on the corresponding graphs. • P
    Operetta High Content Imaging System, supplied by PerkinElmer, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/operetta high content imaging system/product/PerkinElmer
    Average 97 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    operetta high content imaging system - by Bioz Stars, 2021-05
    97/100 stars
      Buy from Supplier

    N/A
    in vitro established from a primary skin sarcoma
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    N/A
    In vitro established from a primary sarcoma of the skin
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    N/A
    established from the primary bladder carcinoma of a 61 year old male 1998
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    N/A
    In vitro etablished from the primary amelanotic melanoma
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    Image Search Results


    Os affects mitochondrial function and morphology. ( a ) HCT116 cells were treated with Os (50 μ M) for 16 h, fixed and viewed under electron microscope (magnification × 25 000) White arrows pointing to mitochondria. ( b ) Relative ATP level was determined in HCT116 cells using the ATP Bioluminescence Assay Kit CLS II (Roche), as described in Materials and Methods. ( c ) A total of 6 × 10 5 HCT116 cells were incubated with 50 μ M of Os for 4 h and ETC-II activity was determined using the Human Complex activity Microplate Assay Kit (Mitosciences). ( d ) Os-treated cells were incubated with the potential-sensitive probe DiOC 6 , and ΔΨ m was analyzed by flow cytometry as described in Materials and Methods. Representative bar graphs were plotted with the G-mean values to determine the fold difference in ΔΨ m between control and Os-treated groups. ( e ) Os-treated cells were loaded with N-nonyl-acridine-orange, and cardiolipin oxidation was analyzed by flow cytometry as described in Materials and Methods. Quantification of G-mean values have been reported on the corresponding graphs. • P

    Journal: Cell Death & Disease

    Article Title: A novel Osmium-based compound targets the mitochondria and triggers ROS-dependent apoptosis in colon carcinoma

    doi: 10.1038/cddis.2013.185

    Figure Lengend Snippet: Os affects mitochondrial function and morphology. ( a ) HCT116 cells were treated with Os (50 μ M) for 16 h, fixed and viewed under electron microscope (magnification × 25 000) White arrows pointing to mitochondria. ( b ) Relative ATP level was determined in HCT116 cells using the ATP Bioluminescence Assay Kit CLS II (Roche), as described in Materials and Methods. ( c ) A total of 6 × 10 5 HCT116 cells were incubated with 50 μ M of Os for 4 h and ETC-II activity was determined using the Human Complex activity Microplate Assay Kit (Mitosciences). ( d ) Os-treated cells were incubated with the potential-sensitive probe DiOC 6 , and ΔΨ m was analyzed by flow cytometry as described in Materials and Methods. Representative bar graphs were plotted with the G-mean values to determine the fold difference in ΔΨ m between control and Os-treated groups. ( e ) Os-treated cells were loaded with N-nonyl-acridine-orange, and cardiolipin oxidation was analyzed by flow cytometry as described in Materials and Methods. Quantification of G-mean values have been reported on the corresponding graphs. • P

    Article Snippet: ATP level was determined using luciferase reagent using the ATP Bioluminescence Assay Kit CLS II (Roche, Penzberg, Germany) according to the manufacturer's instructions.

    Techniques: Microscopy, ATP Bioluminescent Assay, Incubation, Activity Assay, Flow Cytometry, Cytometry