CCL22 ELISA Kits Search Results


92
Bio-Techne corporation mouse ccl22/mdc quantikine elisa kit
Mouse Ccl22/Mdc Quantikine Elisa Kit, supplied by Bio-Techne corporation, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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R&D Systems quantikine human mdc ccl22 elisa kits
Quantikine Human Mdc Ccl22 Elisa Kits, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Cusabio chemokine elisas
Chemokine Elisas, supplied by Cusabio, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Boster Bio paired antibody elisa kits
Paired Antibody Elisa Kits, supplied by Boster Bio, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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R&D Systems human ccl22 mdc elisa kit
A) Illustration of the 3D OTC model supplemented with human skin-tropic (CLA + ) memory (CD45RA - ) CD4 + and CD8 + T cells (ratio 4:1) that were isolated by fluorescence-activated cell sorting (FACS) from hPBMCs of healthy donors. The dermal layer consists of fibrinogen, human fibroblasts, and human T cells. Human keratinocytes overexpressing oncogenes were placed on top to grow 3D OTCs under air-liquid interface conditions for 14 days. Data include experiments with hPBMCs of two independent donors. B) Representative immunohistochemistry stainings of control, G act , Y act , GY act 3D OTCs containing human T cells (anti-CD45, brown). Nuclei were counter-stained using hematoxylin (blue). Black arrows indicate epidermal T cells. E: Epidermis, D: Dermis. Scale bars 100 µm. C) Representative immunofluorescence stainings of control, G act , Y act , GY act 3D OTCs containing T cells (anti-CD45, FITC, green). Nuclei were stained using DAPI (blue). White arrows indicate epidermal T cells. The dotted line marks dermal-epidermal junction. E: Epidermis, D: Dermis. Scale bars 100 µm. D) Quantification of epidermal (left) and dermal (right) CD45 + T cells relative to the total number of T cells per analyzed skin area. N = 3. Statistics: One-way ANOVA. *p<0.05; **p<0.01; ***p<0.001; ****p<0.0001. E) ELISA of 3D OTC media for chemokine levels of <t>CCL22,</t> CCL27 and CXCL10. N = 3. Statistics: One-way ANOVA. *p<0.05; **p<0.01; ***p<0.001.
Human Ccl22 Mdc Elisa Kit, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Cusabio mdc ccl22 elisa kit
A) Illustration of the 3D OTC model supplemented with human skin-tropic (CLA + ) memory (CD45RA - ) CD4 + and CD8 + T cells (ratio 4:1) that were isolated by fluorescence-activated cell sorting (FACS) from hPBMCs of healthy donors. The dermal layer consists of fibrinogen, human fibroblasts, and human T cells. Human keratinocytes overexpressing oncogenes were placed on top to grow 3D OTCs under air-liquid interface conditions for 14 days. Data include experiments with hPBMCs of two independent donors. B) Representative immunohistochemistry stainings of control, G act , Y act , GY act 3D OTCs containing human T cells (anti-CD45, brown). Nuclei were counter-stained using hematoxylin (blue). Black arrows indicate epidermal T cells. E: Epidermis, D: Dermis. Scale bars 100 µm. C) Representative immunofluorescence stainings of control, G act , Y act , GY act 3D OTCs containing T cells (anti-CD45, FITC, green). Nuclei were stained using DAPI (blue). White arrows indicate epidermal T cells. The dotted line marks dermal-epidermal junction. E: Epidermis, D: Dermis. Scale bars 100 µm. D) Quantification of epidermal (left) and dermal (right) CD45 + T cells relative to the total number of T cells per analyzed skin area. N = 3. Statistics: One-way ANOVA. *p<0.05; **p<0.01; ***p<0.001; ****p<0.0001. E) ELISA of 3D OTC media for chemokine levels of <t>CCL22,</t> CCL27 and CXCL10. N = 3. Statistics: One-way ANOVA. *p<0.05; **p<0.01; ***p<0.001.
Mdc Ccl22 Elisa Kit, supplied by Cusabio, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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R&D Systems sensitivity quantikine elisa kits
A) Illustration of the 3D OTC model supplemented with human skin-tropic (CLA + ) memory (CD45RA - ) CD4 + and CD8 + T cells (ratio 4:1) that were isolated by fluorescence-activated cell sorting (FACS) from hPBMCs of healthy donors. The dermal layer consists of fibrinogen, human fibroblasts, and human T cells. Human keratinocytes overexpressing oncogenes were placed on top to grow 3D OTCs under air-liquid interface conditions for 14 days. Data include experiments with hPBMCs of two independent donors. B) Representative immunohistochemistry stainings of control, G act , Y act , GY act 3D OTCs containing human T cells (anti-CD45, brown). Nuclei were counter-stained using hematoxylin (blue). Black arrows indicate epidermal T cells. E: Epidermis, D: Dermis. Scale bars 100 µm. C) Representative immunofluorescence stainings of control, G act , Y act , GY act 3D OTCs containing T cells (anti-CD45, FITC, green). Nuclei were stained using DAPI (blue). White arrows indicate epidermal T cells. The dotted line marks dermal-epidermal junction. E: Epidermis, D: Dermis. Scale bars 100 µm. D) Quantification of epidermal (left) and dermal (right) CD45 + T cells relative to the total number of T cells per analyzed skin area. N = 3. Statistics: One-way ANOVA. *p<0.05; **p<0.01; ***p<0.001; ****p<0.0001. E) ELISA of 3D OTC media for chemokine levels of <t>CCL22,</t> CCL27 and CXCL10. N = 3. Statistics: One-way ANOVA. *p<0.05; **p<0.01; ***p<0.001.
Sensitivity Quantikine Elisa Kits, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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MultiSciences Biotech Co Ltd ccl22 elisa kit
A) Illustration of the 3D OTC model supplemented with human skin-tropic (CLA + ) memory (CD45RA - ) CD4 + and CD8 + T cells (ratio 4:1) that were isolated by fluorescence-activated cell sorting (FACS) from hPBMCs of healthy donors. The dermal layer consists of fibrinogen, human fibroblasts, and human T cells. Human keratinocytes overexpressing oncogenes were placed on top to grow 3D OTCs under air-liquid interface conditions for 14 days. Data include experiments with hPBMCs of two independent donors. B) Representative immunohistochemistry stainings of control, G act , Y act , GY act 3D OTCs containing human T cells (anti-CD45, brown). Nuclei were counter-stained using hematoxylin (blue). Black arrows indicate epidermal T cells. E: Epidermis, D: Dermis. Scale bars 100 µm. C) Representative immunofluorescence stainings of control, G act , Y act , GY act 3D OTCs containing T cells (anti-CD45, FITC, green). Nuclei were stained using DAPI (blue). White arrows indicate epidermal T cells. The dotted line marks dermal-epidermal junction. E: Epidermis, D: Dermis. Scale bars 100 µm. D) Quantification of epidermal (left) and dermal (right) CD45 + T cells relative to the total number of T cells per analyzed skin area. N = 3. Statistics: One-way ANOVA. *p<0.05; **p<0.01; ***p<0.001; ****p<0.0001. E) ELISA of 3D OTC media for chemokine levels of <t>CCL22,</t> CCL27 and CXCL10. N = 3. Statistics: One-way ANOVA. *p<0.05; **p<0.01; ***p<0.001.
Ccl22 Elisa Kit, supplied by MultiSciences Biotech Co Ltd, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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R&D Systems duoset detection kit
A) Illustration of the 3D OTC model supplemented with human skin-tropic (CLA + ) memory (CD45RA - ) CD4 + and CD8 + T cells (ratio 4:1) that were isolated by fluorescence-activated cell sorting (FACS) from hPBMCs of healthy donors. The dermal layer consists of fibrinogen, human fibroblasts, and human T cells. Human keratinocytes overexpressing oncogenes were placed on top to grow 3D OTCs under air-liquid interface conditions for 14 days. Data include experiments with hPBMCs of two independent donors. B) Representative immunohistochemistry stainings of control, G act , Y act , GY act 3D OTCs containing human T cells (anti-CD45, brown). Nuclei were counter-stained using hematoxylin (blue). Black arrows indicate epidermal T cells. E: Epidermis, D: Dermis. Scale bars 100 µm. C) Representative immunofluorescence stainings of control, G act , Y act , GY act 3D OTCs containing T cells (anti-CD45, FITC, green). Nuclei were stained using DAPI (blue). White arrows indicate epidermal T cells. The dotted line marks dermal-epidermal junction. E: Epidermis, D: Dermis. Scale bars 100 µm. D) Quantification of epidermal (left) and dermal (right) CD45 + T cells relative to the total number of T cells per analyzed skin area. N = 3. Statistics: One-way ANOVA. *p<0.05; **p<0.01; ***p<0.001; ****p<0.0001. E) ELISA of 3D OTC media for chemokine levels of <t>CCL22,</t> CCL27 and CXCL10. N = 3. Statistics: One-way ANOVA. *p<0.05; **p<0.01; ***p<0.001.
Duoset Detection Kit, supplied by R&D Systems, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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RayBiotech inc mouse ccl22/mdc1 elisa kit
A) Illustration of the 3D OTC model supplemented with human skin-tropic (CLA + ) memory (CD45RA - ) CD4 + and CD8 + T cells (ratio 4:1) that were isolated by fluorescence-activated cell sorting (FACS) from hPBMCs of healthy donors. The dermal layer consists of fibrinogen, human fibroblasts, and human T cells. Human keratinocytes overexpressing oncogenes were placed on top to grow 3D OTCs under air-liquid interface conditions for 14 days. Data include experiments with hPBMCs of two independent donors. B) Representative immunohistochemistry stainings of control, G act , Y act , GY act 3D OTCs containing human T cells (anti-CD45, brown). Nuclei were counter-stained using hematoxylin (blue). Black arrows indicate epidermal T cells. E: Epidermis, D: Dermis. Scale bars 100 µm. C) Representative immunofluorescence stainings of control, G act , Y act , GY act 3D OTCs containing T cells (anti-CD45, FITC, green). Nuclei were stained using DAPI (blue). White arrows indicate epidermal T cells. The dotted line marks dermal-epidermal junction. E: Epidermis, D: Dermis. Scale bars 100 µm. D) Quantification of epidermal (left) and dermal (right) CD45 + T cells relative to the total number of T cells per analyzed skin area. N = 3. Statistics: One-way ANOVA. *p<0.05; **p<0.01; ***p<0.001; ****p<0.0001. E) ELISA of 3D OTC media for chemokine levels of <t>CCL22,</t> CCL27 and CXCL10. N = 3. Statistics: One-way ANOVA. *p<0.05; **p<0.01; ***p<0.001.
Mouse Ccl22/Mdc1 Elisa Kit, supplied by RayBiotech inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Beyotime human ccl22 elisa kit
XLEP regulates the expression of chemotactic factors in A549, H1975, and PC9 cells. (a) The CCL2 level in the culture supernatant of A549, H1975, and PC9 cells was determined by ELISA. ∗∗∗ P < 0.001 vs. control group. ### P < 0.001 vs. Gef group. (b) The CCL3 level in the culture supernatant of A549, H1975, and PC9 cells was determined by ELISA. ∗∗∗ P < 0.001 vs. control group. ## P < 0.01 and ### P < 0.001 vs. Gef group. (c) The <t>CCL22</t> level in the culture supernatant of A549, H1975, and PC9 cells and the culture supernatant of weredetermined by ELISA. ∗∗ P < 0.01 and ∗∗∗ P < 0.001 vs. control group. ### P < 0.001 vs. Gef group. ΔΔ P < 0.01 vs. TCM group.
Human Ccl22 Elisa Kit, supplied by Beyotime, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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R&D Systems elisa kits assays
XLEP regulates the expression of chemotactic factors in A549, H1975, and PC9 cells. (a) The CCL2 level in the culture supernatant of A549, H1975, and PC9 cells was determined by ELISA. ∗∗∗ P < 0.001 vs. control group. ### P < 0.001 vs. Gef group. (b) The CCL3 level in the culture supernatant of A549, H1975, and PC9 cells was determined by ELISA. ∗∗∗ P < 0.001 vs. control group. ## P < 0.01 and ### P < 0.001 vs. Gef group. (c) The <t>CCL22</t> level in the culture supernatant of A549, H1975, and PC9 cells and the culture supernatant of weredetermined by ELISA. ∗∗ P < 0.01 and ∗∗∗ P < 0.001 vs. control group. ### P < 0.001 vs. Gef group. ΔΔ P < 0.01 vs. TCM group.
Elisa Kits Assays, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


A) Illustration of the 3D OTC model supplemented with human skin-tropic (CLA + ) memory (CD45RA - ) CD4 + and CD8 + T cells (ratio 4:1) that were isolated by fluorescence-activated cell sorting (FACS) from hPBMCs of healthy donors. The dermal layer consists of fibrinogen, human fibroblasts, and human T cells. Human keratinocytes overexpressing oncogenes were placed on top to grow 3D OTCs under air-liquid interface conditions for 14 days. Data include experiments with hPBMCs of two independent donors. B) Representative immunohistochemistry stainings of control, G act , Y act , GY act 3D OTCs containing human T cells (anti-CD45, brown). Nuclei were counter-stained using hematoxylin (blue). Black arrows indicate epidermal T cells. E: Epidermis, D: Dermis. Scale bars 100 µm. C) Representative immunofluorescence stainings of control, G act , Y act , GY act 3D OTCs containing T cells (anti-CD45, FITC, green). Nuclei were stained using DAPI (blue). White arrows indicate epidermal T cells. The dotted line marks dermal-epidermal junction. E: Epidermis, D: Dermis. Scale bars 100 µm. D) Quantification of epidermal (left) and dermal (right) CD45 + T cells relative to the total number of T cells per analyzed skin area. N = 3. Statistics: One-way ANOVA. *p<0.05; **p<0.01; ***p<0.001; ****p<0.0001. E) ELISA of 3D OTC media for chemokine levels of CCL22, CCL27 and CXCL10. N = 3. Statistics: One-way ANOVA. *p<0.05; **p<0.01; ***p<0.001.

Journal: bioRxiv

Article Title: Hedgehog-Hippo pathway interactions promote T cell exclusion from the tumor microenvironment in basal cell carcinoma

doi: 10.1101/2024.07.07.602398

Figure Lengend Snippet: A) Illustration of the 3D OTC model supplemented with human skin-tropic (CLA + ) memory (CD45RA - ) CD4 + and CD8 + T cells (ratio 4:1) that were isolated by fluorescence-activated cell sorting (FACS) from hPBMCs of healthy donors. The dermal layer consists of fibrinogen, human fibroblasts, and human T cells. Human keratinocytes overexpressing oncogenes were placed on top to grow 3D OTCs under air-liquid interface conditions for 14 days. Data include experiments with hPBMCs of two independent donors. B) Representative immunohistochemistry stainings of control, G act , Y act , GY act 3D OTCs containing human T cells (anti-CD45, brown). Nuclei were counter-stained using hematoxylin (blue). Black arrows indicate epidermal T cells. E: Epidermis, D: Dermis. Scale bars 100 µm. C) Representative immunofluorescence stainings of control, G act , Y act , GY act 3D OTCs containing T cells (anti-CD45, FITC, green). Nuclei were stained using DAPI (blue). White arrows indicate epidermal T cells. The dotted line marks dermal-epidermal junction. E: Epidermis, D: Dermis. Scale bars 100 µm. D) Quantification of epidermal (left) and dermal (right) CD45 + T cells relative to the total number of T cells per analyzed skin area. N = 3. Statistics: One-way ANOVA. *p<0.05; **p<0.01; ***p<0.001; ****p<0.0001. E) ELISA of 3D OTC media for chemokine levels of CCL22, CCL27 and CXCL10. N = 3. Statistics: One-way ANOVA. *p<0.05; **p<0.01; ***p<0.001.

Article Snippet: The human CCL22/MDC ELISA kit was purchased from R&D Systems, Biotechne.

Techniques: Isolation, Fluorescence, FACS, Immunohistochemistry, Control, Staining, Immunofluorescence, Enzyme-linked Immunosorbent Assay

XLEP regulates the expression of chemotactic factors in A549, H1975, and PC9 cells. (a) The CCL2 level in the culture supernatant of A549, H1975, and PC9 cells was determined by ELISA. ∗∗∗ P < 0.001 vs. control group. ### P < 0.001 vs. Gef group. (b) The CCL3 level in the culture supernatant of A549, H1975, and PC9 cells was determined by ELISA. ∗∗∗ P < 0.001 vs. control group. ## P < 0.01 and ### P < 0.001 vs. Gef group. (c) The CCL22 level in the culture supernatant of A549, H1975, and PC9 cells and the culture supernatant of weredetermined by ELISA. ∗∗ P < 0.01 and ∗∗∗ P < 0.001 vs. control group. ### P < 0.001 vs. Gef group. ΔΔ P < 0.01 vs. TCM group.

Journal: Evidence-based Complementary and Alternative Medicine : eCAM

Article Title: Mechanism of Xu Li's Experiential Prescription for the Treatment of EGFR-Positive NSCLC

doi: 10.1155/2020/8787153

Figure Lengend Snippet: XLEP regulates the expression of chemotactic factors in A549, H1975, and PC9 cells. (a) The CCL2 level in the culture supernatant of A549, H1975, and PC9 cells was determined by ELISA. ∗∗∗ P < 0.001 vs. control group. ### P < 0.001 vs. Gef group. (b) The CCL3 level in the culture supernatant of A549, H1975, and PC9 cells was determined by ELISA. ∗∗∗ P < 0.001 vs. control group. ## P < 0.01 and ### P < 0.001 vs. Gef group. (c) The CCL22 level in the culture supernatant of A549, H1975, and PC9 cells and the culture supernatant of weredetermined by ELISA. ∗∗ P < 0.01 and ∗∗∗ P < 0.001 vs. control group. ### P < 0.001 vs. Gef group. ΔΔ P < 0.01 vs. TCM group.

Article Snippet: The levels of CCL2, CCL3, and CCL22 in the culture supernatant of A549, H1975, and PC9 cells were, respectively, determined by ELISA using the human CCL2 ELISA kit, human CCL3 ELISA kit, and human CCL22 ELISA kit (Beyotime Biotechnology Co., Ltd., Shanghai, China).

Techniques: Expressing, Enzyme-linked Immunosorbent Assay, Control