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Image Search Results
Journal: bioRxiv
Article Title: Hedgehog-Hippo pathway interactions promote T cell exclusion from the tumor microenvironment in basal cell carcinoma
doi: 10.1101/2024.07.07.602398
Figure Lengend Snippet: A) Illustration of the 3D OTC model supplemented with human skin-tropic (CLA + ) memory (CD45RA - ) CD4 + and CD8 + T cells (ratio 4:1) that were isolated by fluorescence-activated cell sorting (FACS) from hPBMCs of healthy donors. The dermal layer consists of fibrinogen, human fibroblasts, and human T cells. Human keratinocytes overexpressing oncogenes were placed on top to grow 3D OTCs under air-liquid interface conditions for 14 days. Data include experiments with hPBMCs of two independent donors. B) Representative immunohistochemistry stainings of control, G act , Y act , GY act 3D OTCs containing human T cells (anti-CD45, brown). Nuclei were counter-stained using hematoxylin (blue). Black arrows indicate epidermal T cells. E: Epidermis, D: Dermis. Scale bars 100 µm. C) Representative immunofluorescence stainings of control, G act , Y act , GY act 3D OTCs containing T cells (anti-CD45, FITC, green). Nuclei were stained using DAPI (blue). White arrows indicate epidermal T cells. The dotted line marks dermal-epidermal junction. E: Epidermis, D: Dermis. Scale bars 100 µm. D) Quantification of epidermal (left) and dermal (right) CD45 + T cells relative to the total number of T cells per analyzed skin area. N = 3. Statistics: One-way ANOVA. *p<0.05; **p<0.01; ***p<0.001; ****p<0.0001. E) ELISA of 3D OTC media for chemokine levels of CCL22, CCL27 and CXCL10. N = 3. Statistics: One-way ANOVA. *p<0.05; **p<0.01; ***p<0.001.
Article Snippet: The
Techniques: Isolation, Fluorescence, FACS, Immunohistochemistry, Control, Staining, Immunofluorescence, Enzyme-linked Immunosorbent Assay
Journal: Evidence-based Complementary and Alternative Medicine : eCAM
Article Title: Mechanism of Xu Li's Experiential Prescription for the Treatment of EGFR-Positive NSCLC
doi: 10.1155/2020/8787153
Figure Lengend Snippet: XLEP regulates the expression of chemotactic factors in A549, H1975, and PC9 cells. (a) The CCL2 level in the culture supernatant of A549, H1975, and PC9 cells was determined by ELISA. ∗∗∗ P < 0.001 vs. control group. ### P < 0.001 vs. Gef group. (b) The CCL3 level in the culture supernatant of A549, H1975, and PC9 cells was determined by ELISA. ∗∗∗ P < 0.001 vs. control group. ## P < 0.01 and ### P < 0.001 vs. Gef group. (c) The CCL22 level in the culture supernatant of A549, H1975, and PC9 cells and the culture supernatant of weredetermined by ELISA. ∗∗ P < 0.01 and ∗∗∗ P < 0.001 vs. control group. ### P < 0.001 vs. Gef group. ΔΔ P < 0.01 vs. TCM group.
Article Snippet: The levels of CCL2, CCL3, and CCL22 in the culture supernatant of A549, H1975, and PC9 cells were, respectively, determined by ELISA using the human CCL2 ELISA kit, human CCL3 ELISA kit, and
Techniques: Expressing, Enzyme-linked Immunosorbent Assay, Control