C-110 Search Results


93
Alomone Labs cyppa
Endothelial cell barrier permeability following treatment with a) different concentrations of EtOH or b) the TRPM7 antagonists, NS8593, <t>CyPPA,</t> or SKA-31. *p < 0.05; **p < 0.01. a Mouse BMVECs at Passage 7 were seeded into Corning transwell inserts seated in 24-well plates to construct an in-vitro Blood-Brain Barrier (BBB) model. The cells were allowed to grow in the inserts for three days to form a barrier. The same number of wells without cells were used as background. The wells were then treated with 0 mM, 17.4 mM, 52.2 mM and 104.4 mM EtOH for 24 h, and to determine permeability, 50 ng/μl Evans Blue Dye (EBD) was added into the inserts at the same time and the model was incubated at 37 °C for 24 h. The permeability coefficient was then calculated. P-value was calculated using student’s t test. b Mouse BMVECs at Passage 5 were seeded into Corning transwell inserts seated in 24-well plates to construct an in-vitro Blood-Brain Barrier (BBB) model. The cells were allowed to grow in the inserts for three days to form a barrier (BBB). Wells were then treated with three <t>different</t> <t>Trpm</t> 7 Channel Blockers, NS 8593, CyPPA and SKA-31 at 50 μM for 24 h, and the permeability assay was then performed to determine the effect of TRPM7 blockers on the BBB. P-values were calculated using student’s t test
Cyppa, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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90
Kikkoman Biochemifa lumitester c-110
Endothelial cell barrier permeability following treatment with a) different concentrations of EtOH or b) the TRPM7 antagonists, NS8593, <t>CyPPA,</t> or SKA-31. *p < 0.05; **p < 0.01. a Mouse BMVECs at Passage 7 were seeded into Corning transwell inserts seated in 24-well plates to construct an in-vitro Blood-Brain Barrier (BBB) model. The cells were allowed to grow in the inserts for three days to form a barrier. The same number of wells without cells were used as background. The wells were then treated with 0 mM, 17.4 mM, 52.2 mM and 104.4 mM EtOH for 24 h, and to determine permeability, 50 ng/μl Evans Blue Dye (EBD) was added into the inserts at the same time and the model was incubated at 37 °C for 24 h. The permeability coefficient was then calculated. P-value was calculated using student’s t test. b Mouse BMVECs at Passage 5 were seeded into Corning transwell inserts seated in 24-well plates to construct an in-vitro Blood-Brain Barrier (BBB) model. The cells were allowed to grow in the inserts for three days to form a barrier (BBB). Wells were then treated with three <t>different</t> <t>Trpm</t> 7 Channel Blockers, NS 8593, CyPPA and SKA-31 at 50 μM for 24 h, and the permeability assay was then performed to determine the effect of TRPM7 blockers on the BBB. P-values were calculated using student’s t test
Lumitester C 110, supplied by Kikkoman Biochemifa, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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90
Kikkoman Corporation luminometer lumitester c-110
Endothelial cell barrier permeability following treatment with a) different concentrations of EtOH or b) the TRPM7 antagonists, NS8593, <t>CyPPA,</t> or SKA-31. *p < 0.05; **p < 0.01. a Mouse BMVECs at Passage 7 were seeded into Corning transwell inserts seated in 24-well plates to construct an in-vitro Blood-Brain Barrier (BBB) model. The cells were allowed to grow in the inserts for three days to form a barrier. The same number of wells without cells were used as background. The wells were then treated with 0 mM, 17.4 mM, 52.2 mM and 104.4 mM EtOH for 24 h, and to determine permeability, 50 ng/μl Evans Blue Dye (EBD) was added into the inserts at the same time and the model was incubated at 37 °C for 24 h. The permeability coefficient was then calculated. P-value was calculated using student’s t test. b Mouse BMVECs at Passage 5 were seeded into Corning transwell inserts seated in 24-well plates to construct an in-vitro Blood-Brain Barrier (BBB) model. The cells were allowed to grow in the inserts for three days to form a barrier (BBB). Wells were then treated with three <t>different</t> <t>Trpm</t> 7 Channel Blockers, NS 8593, CyPPA and SKA-31 at 50 μM for 24 h, and the permeability assay was then performed to determine the effect of TRPM7 blockers on the BBB. P-values were calculated using student’s t test
Luminometer Lumitester C 110, supplied by Kikkoman Corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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90
Applied Engineering Inc ionisation chamber c110 (0.6)
Endothelial cell barrier permeability following treatment with a) different concentrations of EtOH or b) the TRPM7 antagonists, NS8593, <t>CyPPA,</t> or SKA-31. *p < 0.05; **p < 0.01. a Mouse BMVECs at Passage 7 were seeded into Corning transwell inserts seated in 24-well plates to construct an in-vitro Blood-Brain Barrier (BBB) model. The cells were allowed to grow in the inserts for three days to form a barrier. The same number of wells without cells were used as background. The wells were then treated with 0 mM, 17.4 mM, 52.2 mM and 104.4 mM EtOH for 24 h, and to determine permeability, 50 ng/μl Evans Blue Dye (EBD) was added into the inserts at the same time and the model was incubated at 37 °C for 24 h. The permeability coefficient was then calculated. P-value was calculated using student’s t test. b Mouse BMVECs at Passage 5 were seeded into Corning transwell inserts seated in 24-well plates to construct an in-vitro Blood-Brain Barrier (BBB) model. The cells were allowed to grow in the inserts for three days to form a barrier (BBB). Wells were then treated with three <t>different</t> <t>Trpm</t> 7 Channel Blockers, NS 8593, CyPPA and SKA-31 at 50 μM for 24 h, and the permeability assay was then performed to determine the effect of TRPM7 blockers on the BBB. P-values were calculated using student’s t test
Ionisation Chamber C110 (0.6), supplied by Applied Engineering Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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90
Isogen Life Science kikkoman lumitester c-110
Endothelial cell barrier permeability following treatment with a) different concentrations of EtOH or b) the TRPM7 antagonists, NS8593, <t>CyPPA,</t> or SKA-31. *p < 0.05; **p < 0.01. a Mouse BMVECs at Passage 7 were seeded into Corning transwell inserts seated in 24-well plates to construct an in-vitro Blood-Brain Barrier (BBB) model. The cells were allowed to grow in the inserts for three days to form a barrier. The same number of wells without cells were used as background. The wells were then treated with 0 mM, 17.4 mM, 52.2 mM and 104.4 mM EtOH for 24 h, and to determine permeability, 50 ng/μl Evans Blue Dye (EBD) was added into the inserts at the same time and the model was incubated at 37 °C for 24 h. The permeability coefficient was then calculated. P-value was calculated using student’s t test. b Mouse BMVECs at Passage 5 were seeded into Corning transwell inserts seated in 24-well plates to construct an in-vitro Blood-Brain Barrier (BBB) model. The cells were allowed to grow in the inserts for three days to form a barrier (BBB). Wells were then treated with three <t>different</t> <t>Trpm</t> 7 Channel Blockers, NS 8593, CyPPA and SKA-31 at 50 μM for 24 h, and the permeability assay was then performed to determine the effect of TRPM7 blockers on the BBB. P-values were calculated using student’s t test
Kikkoman Lumitester C 110, supplied by Isogen Life Science, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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90
AGC Inc polycarbonate substrate carboglass (registered trademark) c-110
Endothelial cell barrier permeability following treatment with a) different concentrations of EtOH or b) the TRPM7 antagonists, NS8593, <t>CyPPA,</t> or SKA-31. *p < 0.05; **p < 0.01. a Mouse BMVECs at Passage 7 were seeded into Corning transwell inserts seated in 24-well plates to construct an in-vitro Blood-Brain Barrier (BBB) model. The cells were allowed to grow in the inserts for three days to form a barrier. The same number of wells without cells were used as background. The wells were then treated with 0 mM, 17.4 mM, 52.2 mM and 104.4 mM EtOH for 24 h, and to determine permeability, 50 ng/μl Evans Blue Dye (EBD) was added into the inserts at the same time and the model was incubated at 37 °C for 24 h. The permeability coefficient was then calculated. P-value was calculated using student’s t test. b Mouse BMVECs at Passage 5 were seeded into Corning transwell inserts seated in 24-well plates to construct an in-vitro Blood-Brain Barrier (BBB) model. The cells were allowed to grow in the inserts for three days to form a barrier (BBB). Wells were then treated with three <t>different</t> <t>Trpm</t> 7 Channel Blockers, NS 8593, CyPPA and SKA-31 at 50 μM for 24 h, and the permeability assay was then performed to determine the effect of TRPM7 blockers on the BBB. P-values were calculated using student’s t test
Polycarbonate Substrate Carboglass (Registered Trademark) C 110, supplied by AGC Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
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90
Sairan Co cardiopulmonary monitoring device sairan model c110
Endothelial cell barrier permeability following treatment with a) different concentrations of EtOH or b) the TRPM7 antagonists, NS8593, <t>CyPPA,</t> or SKA-31. *p < 0.05; **p < 0.01. a Mouse BMVECs at Passage 7 were seeded into Corning transwell inserts seated in 24-well plates to construct an in-vitro Blood-Brain Barrier (BBB) model. The cells were allowed to grow in the inserts for three days to form a barrier. The same number of wells without cells were used as background. The wells were then treated with 0 mM, 17.4 mM, 52.2 mM and 104.4 mM EtOH for 24 h, and to determine permeability, 50 ng/μl Evans Blue Dye (EBD) was added into the inserts at the same time and the model was incubated at 37 °C for 24 h. The permeability coefficient was then calculated. P-value was calculated using student’s t test. b Mouse BMVECs at Passage 5 were seeded into Corning transwell inserts seated in 24-well plates to construct an in-vitro Blood-Brain Barrier (BBB) model. The cells were allowed to grow in the inserts for three days to form a barrier (BBB). Wells were then treated with three <t>different</t> <t>Trpm</t> 7 Channel Blockers, NS 8593, CyPPA and SKA-31 at 50 μM for 24 h, and the permeability assay was then performed to determine the effect of TRPM7 blockers on the BBB. P-values were calculated using student’s t test
Cardiopulmonary Monitoring Device Sairan Model C110, supplied by Sairan Co, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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90
JENOPTIK Inc climate chamber c110/200
Endothelial cell barrier permeability following treatment with a) different concentrations of EtOH or b) the TRPM7 antagonists, NS8593, <t>CyPPA,</t> or SKA-31. *p < 0.05; **p < 0.01. a Mouse BMVECs at Passage 7 were seeded into Corning transwell inserts seated in 24-well plates to construct an in-vitro Blood-Brain Barrier (BBB) model. The cells were allowed to grow in the inserts for three days to form a barrier. The same number of wells without cells were used as background. The wells were then treated with 0 mM, 17.4 mM, 52.2 mM and 104.4 mM EtOH for 24 h, and to determine permeability, 50 ng/μl Evans Blue Dye (EBD) was added into the inserts at the same time and the model was incubated at 37 °C for 24 h. The permeability coefficient was then calculated. P-value was calculated using student’s t test. b Mouse BMVECs at Passage 5 were seeded into Corning transwell inserts seated in 24-well plates to construct an in-vitro Blood-Brain Barrier (BBB) model. The cells were allowed to grow in the inserts for three days to form a barrier (BBB). Wells were then treated with three <t>different</t> <t>Trpm</t> 7 Channel Blockers, NS 8593, CyPPA and SKA-31 at 50 μM for 24 h, and the permeability assay was then performed to determine the effect of TRPM7 blockers on the BBB. P-values were calculated using student’s t test
Climate Chamber C110/200, supplied by JENOPTIK Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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90
Imerys Minerals ground calcium carbonate carbital® c110
Endothelial cell barrier permeability following treatment with a) different concentrations of EtOH or b) the TRPM7 antagonists, NS8593, <t>CyPPA,</t> or SKA-31. *p < 0.05; **p < 0.01. a Mouse BMVECs at Passage 7 were seeded into Corning transwell inserts seated in 24-well plates to construct an in-vitro Blood-Brain Barrier (BBB) model. The cells were allowed to grow in the inserts for three days to form a barrier. The same number of wells without cells were used as background. The wells were then treated with 0 mM, 17.4 mM, 52.2 mM and 104.4 mM EtOH for 24 h, and to determine permeability, 50 ng/μl Evans Blue Dye (EBD) was added into the inserts at the same time and the model was incubated at 37 °C for 24 h. The permeability coefficient was then calculated. P-value was calculated using student’s t test. b Mouse BMVECs at Passage 5 were seeded into Corning transwell inserts seated in 24-well plates to construct an in-vitro Blood-Brain Barrier (BBB) model. The cells were allowed to grow in the inserts for three days to form a barrier (BBB). Wells were then treated with three <t>different</t> <t>Trpm</t> 7 Channel Blockers, NS 8593, CyPPA and SKA-31 at 50 μM for 24 h, and the permeability assay was then performed to determine the effect of TRPM7 blockers on the BBB. P-values were calculated using student’s t test
Ground Calcium Carbonate Carbital® C110, supplied by Imerys Minerals, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Toho Tenax Europe carbon fibers ht c110
Endothelial cell barrier permeability following treatment with a) different concentrations of EtOH or b) the TRPM7 antagonists, NS8593, <t>CyPPA,</t> or SKA-31. *p < 0.05; **p < 0.01. a Mouse BMVECs at Passage 7 were seeded into Corning transwell inserts seated in 24-well plates to construct an in-vitro Blood-Brain Barrier (BBB) model. The cells were allowed to grow in the inserts for three days to form a barrier. The same number of wells without cells were used as background. The wells were then treated with 0 mM, 17.4 mM, 52.2 mM and 104.4 mM EtOH for 24 h, and to determine permeability, 50 ng/μl Evans Blue Dye (EBD) was added into the inserts at the same time and the model was incubated at 37 °C for 24 h. The permeability coefficient was then calculated. P-value was calculated using student’s t test. b Mouse BMVECs at Passage 5 were seeded into Corning transwell inserts seated in 24-well plates to construct an in-vitro Blood-Brain Barrier (BBB) model. The cells were allowed to grow in the inserts for three days to form a barrier (BBB). Wells were then treated with three <t>different</t> <t>Trpm</t> 7 Channel Blockers, NS 8593, CyPPA and SKA-31 at 50 μM for 24 h, and the permeability assay was then performed to determine the effect of TRPM7 blockers on the BBB. P-values were calculated using student’s t test
Carbon Fibers Ht C110, supplied by Toho Tenax Europe, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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RADWAG Balances & Scales thermob lance m c 110/np, radom, poland
Endothelial cell barrier permeability following treatment with a) different concentrations of EtOH or b) the TRPM7 antagonists, NS8593, <t>CyPPA,</t> or SKA-31. *p < 0.05; **p < 0.01. a Mouse BMVECs at Passage 7 were seeded into Corning transwell inserts seated in 24-well plates to construct an in-vitro Blood-Brain Barrier (BBB) model. The cells were allowed to grow in the inserts for three days to form a barrier. The same number of wells without cells were used as background. The wells were then treated with 0 mM, 17.4 mM, 52.2 mM and 104.4 mM EtOH for 24 h, and to determine permeability, 50 ng/μl Evans Blue Dye (EBD) was added into the inserts at the same time and the model was incubated at 37 °C for 24 h. The permeability coefficient was then calculated. P-value was calculated using student’s t test. b Mouse BMVECs at Passage 5 were seeded into Corning transwell inserts seated in 24-well plates to construct an in-vitro Blood-Brain Barrier (BBB) model. The cells were allowed to grow in the inserts for three days to form a barrier (BBB). Wells were then treated with three <t>different</t> <t>Trpm</t> 7 Channel Blockers, NS 8593, CyPPA and SKA-31 at 50 μM for 24 h, and the permeability assay was then performed to determine the effect of TRPM7 blockers on the BBB. P-values were calculated using student’s t test
Thermob Lance M C 110/Np, Radom, Poland, supplied by RADWAG Balances & Scales, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
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90
Advanced Photonix Inc pdb-c110
Endothelial cell barrier permeability following treatment with a) different concentrations of EtOH or b) the TRPM7 antagonists, NS8593, <t>CyPPA,</t> or SKA-31. *p < 0.05; **p < 0.01. a Mouse BMVECs at Passage 7 were seeded into Corning transwell inserts seated in 24-well plates to construct an in-vitro Blood-Brain Barrier (BBB) model. The cells were allowed to grow in the inserts for three days to form a barrier. The same number of wells without cells were used as background. The wells were then treated with 0 mM, 17.4 mM, 52.2 mM and 104.4 mM EtOH for 24 h, and to determine permeability, 50 ng/μl Evans Blue Dye (EBD) was added into the inserts at the same time and the model was incubated at 37 °C for 24 h. The permeability coefficient was then calculated. P-value was calculated using student’s t test. b Mouse BMVECs at Passage 5 were seeded into Corning transwell inserts seated in 24-well plates to construct an in-vitro Blood-Brain Barrier (BBB) model. The cells were allowed to grow in the inserts for three days to form a barrier (BBB). Wells were then treated with three <t>different</t> <t>Trpm</t> 7 Channel Blockers, NS 8593, CyPPA and SKA-31 at 50 μM for 24 h, and the permeability assay was then performed to determine the effect of TRPM7 blockers on the BBB. P-values were calculated using student’s t test
Pdb C110, supplied by Advanced Photonix Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Endothelial cell barrier permeability following treatment with a) different concentrations of EtOH or b) the TRPM7 antagonists, NS8593, CyPPA, or SKA-31. *p < 0.05; **p < 0.01. a Mouse BMVECs at Passage 7 were seeded into Corning transwell inserts seated in 24-well plates to construct an in-vitro Blood-Brain Barrier (BBB) model. The cells were allowed to grow in the inserts for three days to form a barrier. The same number of wells without cells were used as background. The wells were then treated with 0 mM, 17.4 mM, 52.2 mM and 104.4 mM EtOH for 24 h, and to determine permeability, 50 ng/μl Evans Blue Dye (EBD) was added into the inserts at the same time and the model was incubated at 37 °C for 24 h. The permeability coefficient was then calculated. P-value was calculated using student’s t test. b Mouse BMVECs at Passage 5 were seeded into Corning transwell inserts seated in 24-well plates to construct an in-vitro Blood-Brain Barrier (BBB) model. The cells were allowed to grow in the inserts for three days to form a barrier (BBB). Wells were then treated with three different Trpm 7 Channel Blockers, NS 8593, CyPPA and SKA-31 at 50 μM for 24 h, and the permeability assay was then performed to determine the effect of TRPM7 blockers on the BBB. P-values were calculated using student’s t test

Journal: Journal of neuroimmune pharmacology : the official journal of the Society on NeuroImmune Pharmacology

Article Title: Ethanol’s Effects on Transient Receptor Potential Channel Expression in Brain Microvascular Endothelial Cells

doi: 10.1007/s11481-018-9796-3

Figure Lengend Snippet: Endothelial cell barrier permeability following treatment with a) different concentrations of EtOH or b) the TRPM7 antagonists, NS8593, CyPPA, or SKA-31. *p < 0.05; **p < 0.01. a Mouse BMVECs at Passage 7 were seeded into Corning transwell inserts seated in 24-well plates to construct an in-vitro Blood-Brain Barrier (BBB) model. The cells were allowed to grow in the inserts for three days to form a barrier. The same number of wells without cells were used as background. The wells were then treated with 0 mM, 17.4 mM, 52.2 mM and 104.4 mM EtOH for 24 h, and to determine permeability, 50 ng/μl Evans Blue Dye (EBD) was added into the inserts at the same time and the model was incubated at 37 °C for 24 h. The permeability coefficient was then calculated. P-value was calculated using student’s t test. b Mouse BMVECs at Passage 5 were seeded into Corning transwell inserts seated in 24-well plates to construct an in-vitro Blood-Brain Barrier (BBB) model. The cells were allowed to grow in the inserts for three days to form a barrier (BBB). Wells were then treated with three different Trpm 7 Channel Blockers, NS 8593, CyPPA and SKA-31 at 50 μM for 24 h, and the permeability assay was then performed to determine the effect of TRPM7 blockers on the BBB. P-values were calculated using student’s t test

Article Snippet: For Trpm 7 inhibition, three different Trpm 7 Channel Blockers, NS 8593, CyPPA and SKA-31 (Alomone Labs, Jerusalem, Israel) were added to the inserts at 50 μM, together with a tracer Sodium Fluorescein (NaF) at 10 μg/mL, and incubated in a humidified cell culture incubator at 37 °C with 5% CO 2 for 24 h. One hundred microliter samples from both the upper insert and the lower chamber were transferred to a clear 96-well plate.

Techniques: Permeability, Construct, In Vitro, Incubation