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Rockland Immunochemicals
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Addgene inc
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Quantum Detectors Ltd
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Alomone Labs
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Revvity
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Rockland Immunochemicals
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Zymo Research
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Gold Biotechnology Inc
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European Directorate for the Quality of Medicines and HealthCare
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Larodan
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Image Search Results
Journal: Nature communications
Article Title: Trim33 masks a non-transcriptional function of E2f4 in replication fork progression.
doi: 10.1038/s41467-023-40847-0
Figure Lengend Snippet: Fig. 1 | Deletion of Trim33 abates replicative stress. a Immunoblotting analysis of p19/Nras Trim33-WT and Trim33-KO cell lines; n = 3. b Analysis of the RNA-seq data for two Trim33-KO cell lines; n = 3. Venn diagram shows the number of commonly deregulated genes (p adj <0.01, any log2FC) for the two knockout cell lines com- pared to Trim33-WT cells. Top three enriched KEGG pathways are shown (analyzed by Enrichr70). c DNA fiber assays in Trim33-WT and Trim33-KO cells expressing Myc or a Ctrl vector; n = 2. 100 fibers were measured per sample; scale bar = 1 μm. Sig- nificance was determined using Kruskal–Wallis test and Dunn’s multiple compar- isons. d Immunoblotting analysis in Trim33-WT and Trim33-KO cells expressing Myc or Ctrl vector; n = 3. e DNA fiber assays in Trim33-WT and Trim33-KO cells, unchallenged (Ctrl) or released from a 4-h hydroxyurea (HU) treatment (HU-rel); n = 3. 100 fibers were measured per sample; significance was determined as in c). Scale bar = 1 μm. f EdU incorporation analysis in Ctrl and HU-treated Trim33-WT
Article Snippet:
Techniques: Western Blot, RNA Sequencing, Knock-Out, Expressing, Plasmid Preparation
Journal: Nature communications
Article Title: Trim33 masks a non-transcriptional function of E2f4 in replication fork progression.
doi: 10.1038/s41467-023-40847-0
Figure Lengend Snippet: Fig. 3 | Deletion of Trim33 promotes E2f4 interactions with chromatin and with the Recql helicase. a Genome browser tracks of E2f4 ChIP-seq in p19/Nras Trim33- WT and Trim33-KO cells; n = 2. b Quantification of ChIP-seq tag coverage at E2f4 peaks in p19/Nras Trim33-WT and Trim33-KO cells. c Relationship between changes (log2FC) in E2f4 binding (normalized E2f4 ChIP-seq tags) and mRNA expression (log2FC) of the encoded gene for p19/Nras Trim33-KO vs Trim33-WT cells. Each dot is a bin of 5 genes sorted by the change in mRNA levels. Trendlines are derived from linear regression analysis. d LC-MS/MS analysis of E2f4 interactome in p19/Nras Trim33-WT and Trim33-KO cells. Known interaction partners are highlighted in blue. e PLA analysis of Recql-E2f4 association in p19/Nras Trim33-WT and Trim33- KO cells stably expressing HA-tagged E2f4 or vector Ctrl; from left, n = 49,49,50,50
Article Snippet:
Techniques: ChIP-sequencing, Binding Assay, Expressing, Derivative Assay, Liquid Chromatography with Mass Spectroscopy, Stable Transfection, Plasmid Preparation