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trpc1 detection  (Alomone Labs)
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Alomone Labs trpc1 detection
( A ) qRT-PCR analysis of KCa3.1 mRNA expression in MCF-7 cells transfected with scrambled siRNA (siCTL), siRNA directed against KCa3.1 (siKCa3.1), siRNA directed against <t>TRPC1</t> (siTRPC1). The graph shows KCa3.1 mRNA expression normalized to β-actin mRNA expression ( n = 4). ( B ) qRT-PCR analysis of TRPC1 expression level in MCF-7 cells transfected with siCTL, siKCa3.1 or siTRPC1. The graph shows TRPC1 mRNA expression normalized to b-actin mRNA expression ( n = 4). ( C ) Representative western blot showing the effect of siRNAs directed against KCa3.1 and TRPC1 on the protein level of KCa3.1. ( D ) Representative western blot showing the effect of siRNAs directed against KCa3.1 and TRPC1 on the protein level of TRPC1. ( E ) Analysis of MCF-7 cell proliferation transfected with siCTL, siKCa3.1, siTRPC1 or both siKCa3.1 and siTRPC1. Cell proliferation is measured 72 h post-transfection. Values are reported as mean ± SEM normalized to the control ( n = 4). ** p < 0.01, *** p < 0.001, n.s.: not significant.
Trpc1 Detection, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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( A ) qRT-PCR analysis of KCa3.1 mRNA expression in MCF-7 cells transfected with scrambled siRNA (siCTL), siRNA directed against KCa3.1 (siKCa3.1), siRNA directed against TRPC1 (siTRPC1). The graph shows KCa3.1 mRNA expression normalized to β-actin mRNA expression ( n = 4). ( B ) qRT-PCR analysis of TRPC1 expression level in MCF-7 cells transfected with siCTL, siKCa3.1 or siTRPC1. The graph shows TRPC1 mRNA expression normalized to b-actin mRNA expression ( n = 4). ( C ) Representative western blot showing the effect of siRNAs directed against KCa3.1 and TRPC1 on the protein level of KCa3.1. ( D ) Representative western blot showing the effect of siRNAs directed against KCa3.1 and TRPC1 on the protein level of TRPC1. ( E ) Analysis of MCF-7 cell proliferation transfected with siCTL, siKCa3.1, siTRPC1 or both siKCa3.1 and siTRPC1. Cell proliferation is measured 72 h post-transfection. Values are reported as mean ± SEM normalized to the control ( n = 4). ** p < 0.01, *** p < 0.001, n.s.: not significant.

Journal: Oncotarget

Article Title: Functional cooperation between KCa3.1 and TRPC1 channels in human breast cancer: Role in cell proliferation and patient prognosis

doi: 10.18632/oncotarget.9261

Figure Lengend Snippet: ( A ) qRT-PCR analysis of KCa3.1 mRNA expression in MCF-7 cells transfected with scrambled siRNA (siCTL), siRNA directed against KCa3.1 (siKCa3.1), siRNA directed against TRPC1 (siTRPC1). The graph shows KCa3.1 mRNA expression normalized to β-actin mRNA expression ( n = 4). ( B ) qRT-PCR analysis of TRPC1 expression level in MCF-7 cells transfected with siCTL, siKCa3.1 or siTRPC1. The graph shows TRPC1 mRNA expression normalized to b-actin mRNA expression ( n = 4). ( C ) Representative western blot showing the effect of siRNAs directed against KCa3.1 and TRPC1 on the protein level of KCa3.1. ( D ) Representative western blot showing the effect of siRNAs directed against KCa3.1 and TRPC1 on the protein level of TRPC1. ( E ) Analysis of MCF-7 cell proliferation transfected with siCTL, siKCa3.1, siTRPC1 or both siKCa3.1 and siTRPC1. Cell proliferation is measured 72 h post-transfection. Values are reported as mean ± SEM normalized to the control ( n = 4). ** p < 0.01, *** p < 0.001, n.s.: not significant.

Article Snippet: Anti-TRPC1 anti-body (ACC-010, Alomone, Jeruzalem, Israel, at 1:200) was used for TRPC1 detection.

Techniques: Quantitative RT-PCR, Expressing, Transfection, Western Blot

MCF-7 cells were transfected using Amaxa with either control siRNA (siCTL), KCa3.1 siRNA (siKCa3.1), TRPC1 siRNA (siTRPC1) or both TRPC1/KCa3.1 siRNAs (siTRPC1/siKCa3.1), and then cultured in EMEM medium with 5% FBS for 72 h. After staining with propidium iodide, cell cycle distribution (G0/G1, S and G2/M phases) was examined by flow cytometry. The graph represents the percentage of cells in different phases under control condition or KCa3.1 or TRPC1 knockdown conditions ( n = 3). Insets show raw data from the FACS acquisition software. Values are reported as mean ± SEM. **, p < 0.01, n.s.: not significant.

Journal: Oncotarget

Article Title: Functional cooperation between KCa3.1 and TRPC1 channels in human breast cancer: Role in cell proliferation and patient prognosis

doi: 10.18632/oncotarget.9261

Figure Lengend Snippet: MCF-7 cells were transfected using Amaxa with either control siRNA (siCTL), KCa3.1 siRNA (siKCa3.1), TRPC1 siRNA (siTRPC1) or both TRPC1/KCa3.1 siRNAs (siTRPC1/siKCa3.1), and then cultured in EMEM medium with 5% FBS for 72 h. After staining with propidium iodide, cell cycle distribution (G0/G1, S and G2/M phases) was examined by flow cytometry. The graph represents the percentage of cells in different phases under control condition or KCa3.1 or TRPC1 knockdown conditions ( n = 3). Insets show raw data from the FACS acquisition software. Values are reported as mean ± SEM. **, p < 0.01, n.s.: not significant.

Article Snippet: Anti-TRPC1 anti-body (ACC-010, Alomone, Jeruzalem, Israel, at 1:200) was used for TRPC1 detection.

Techniques: Transfection, Cell Culture, Staining, Flow Cytometry, Software

( A ) qRT-PCR analysis of KCa3.1 mRNA expression level in synchronized cells. ( B ) qRT-PCR analysis of TRPC1 mRNA expression level in synchronized cells. ( C ) Representative western blot showing the expression level of KCa3.1 in synchronized cells. ( D ) Representative western blot showing the expression level of TRPC1 in synchronized cells. Cell synchronization was achieved by 24 h treatment of cells by either serum- and phenol red-free medium (mid-G1) or by complete medium supplemented with 2 mM thymidine (end-G1). Values are reported as mean ± SEM normalized to control. *** p < 0.001.

Journal: Oncotarget

Article Title: Functional cooperation between KCa3.1 and TRPC1 channels in human breast cancer: Role in cell proliferation and patient prognosis

doi: 10.18632/oncotarget.9261

Figure Lengend Snippet: ( A ) qRT-PCR analysis of KCa3.1 mRNA expression level in synchronized cells. ( B ) qRT-PCR analysis of TRPC1 mRNA expression level in synchronized cells. ( C ) Representative western blot showing the expression level of KCa3.1 in synchronized cells. ( D ) Representative western blot showing the expression level of TRPC1 in synchronized cells. Cell synchronization was achieved by 24 h treatment of cells by either serum- and phenol red-free medium (mid-G1) or by complete medium supplemented with 2 mM thymidine (end-G1). Values are reported as mean ± SEM normalized to control. *** p < 0.001.

Article Snippet: Anti-TRPC1 anti-body (ACC-010, Alomone, Jeruzalem, Israel, at 1:200) was used for TRPC1 detection.

Techniques: Quantitative RT-PCR, Expressing, Western Blot

( A ) MCF-7 cell lysates were subjected to immunoprecipitation with anti-KCa3.1. KCa3.1 and TRPC1 were identified by Western blot analysis using anti-KCa3.1 and anti-TRPC1 antibodies. Cells were treated or not treated with methyl-β-cyclodextrin. ( B ) Quantification of the KCa3.1 and TRPC1 interaction in MCF-7 cells treated or not treated with methyl-β-cyclodextrin. *** p < 0.001.

Journal: Oncotarget

Article Title: Functional cooperation between KCa3.1 and TRPC1 channels in human breast cancer: Role in cell proliferation and patient prognosis

doi: 10.18632/oncotarget.9261

Figure Lengend Snippet: ( A ) MCF-7 cell lysates were subjected to immunoprecipitation with anti-KCa3.1. KCa3.1 and TRPC1 were identified by Western blot analysis using anti-KCa3.1 and anti-TRPC1 antibodies. Cells were treated or not treated with methyl-β-cyclodextrin. ( B ) Quantification of the KCa3.1 and TRPC1 interaction in MCF-7 cells treated or not treated with methyl-β-cyclodextrin. *** p < 0.001.

Article Snippet: Anti-TRPC1 anti-body (ACC-010, Alomone, Jeruzalem, Israel, at 1:200) was used for TRPC1 detection.

Techniques: Immunoprecipitation, Western Blot