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    Alomone Labs trpv4
    <t>TRPV4</t> activation led to enhanced expression of TNF receptor 1 (TNFR1). A Immunoblotting analysis showing that pre-injection of GSK101 enhanced the expression of TNFR1 in the retina, compared with the control group. B Bar chart summarizing mean expression levels of TNFR1 under different conditions. n = 5, p
    Trpv4, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    TRPV4 activation led to enhanced expression of TNF receptor 1 (TNFR1). A Immunoblotting analysis showing that pre-injection of GSK101 enhanced the expression of TNFR1 in the retina, compared with the control group. B Bar chart summarizing mean expression levels of TNFR1 under different conditions. n = 5, p

    Journal: Journal of Neuroinflammation

    Article Title: TRPV4-induced Müller cell gliosis and TNF-α elevation-mediated retinal ganglion cell apoptosis in glaucomatous rats via JAK2/STAT3/NF-κB pathway

    doi: 10.1186/s12974-021-02315-8

    Figure Lengend Snippet: TRPV4 activation led to enhanced expression of TNF receptor 1 (TNFR1). A Immunoblotting analysis showing that pre-injection of GSK101 enhanced the expression of TNFR1 in the retina, compared with the control group. B Bar chart summarizing mean expression levels of TNFR1 under different conditions. n = 5, p

    Article Snippet: As negative controls, TRPV4 and GFAP antibodies were pre-adsorbed with TRPV4 blocking peptide (Alomone Labs) and GFAP blocking peptide (Bioss), respectively.

    Techniques: Activation Assay, Expressing, Injection

    TRPV4 activation led to increased phosphorylation of JAK2 and STAT3, thereby inducing NF-κB p65 translocation from the cytoplasm into the nucleus. A , C Immunoblotting analysis showing that GSK101 treatment increased the phosphorylation of STAT3 and JAK2, compared with the control group, but had no effect on the protein levels of JAK2 and STAT3 ( C ). B , D Bar charts summarizing mean expression levels of phosphorylated STAT3/STAT ( B ) and phosphorylated JAK2/JAK2 ( D ) under different conditions. n = 3 for all groups, * p

    Journal: Journal of Neuroinflammation

    Article Title: TRPV4-induced Müller cell gliosis and TNF-α elevation-mediated retinal ganglion cell apoptosis in glaucomatous rats via JAK2/STAT3/NF-κB pathway

    doi: 10.1186/s12974-021-02315-8

    Figure Lengend Snippet: TRPV4 activation led to increased phosphorylation of JAK2 and STAT3, thereby inducing NF-κB p65 translocation from the cytoplasm into the nucleus. A , C Immunoblotting analysis showing that GSK101 treatment increased the phosphorylation of STAT3 and JAK2, compared with the control group, but had no effect on the protein levels of JAK2 and STAT3 ( C ). B , D Bar charts summarizing mean expression levels of phosphorylated STAT3/STAT ( B ) and phosphorylated JAK2/JAK2 ( D ) under different conditions. n = 3 for all groups, * p

    Article Snippet: As negative controls, TRPV4 and GFAP antibodies were pre-adsorbed with TRPV4 blocking peptide (Alomone Labs) and GFAP blocking peptide (Bioss), respectively.

    Techniques: Activation Assay, Translocation Assay, Expressing

    Müller cells express functional TRPV4. A Immunofluorescence images show that cells with red autofluorescence are Müller cells. A1 Immunofluorescence images show cells with red autofluorescence in rat retinal vertical slices acquired from TdTomato transgenic mouse retina (Tomato). A2 Immunofluorescence images show glutamine synthetase (GS) protein staining (green) in the slices depicted in A1 . A3 Immunofluorescence images show DAPI (blue) staining in the slices depicted in A1 . A4 shows merged images. Scale bar: 50 µm. B Representative trace recorded from a Müller cell identified by spontaneous red fluorescence, showing that perfusion with GSK101 (10 μM) caused significant depolarization of Müller membrane potential. C Bar chart showing GSK101-induced depolarization of Müller cell membrane potential. n = 4. * p

    Journal: Journal of Neuroinflammation

    Article Title: TRPV4-induced Müller cell gliosis and TNF-α elevation-mediated retinal ganglion cell apoptosis in glaucomatous rats via JAK2/STAT3/NF-κB pathway

    doi: 10.1186/s12974-021-02315-8

    Figure Lengend Snippet: Müller cells express functional TRPV4. A Immunofluorescence images show that cells with red autofluorescence are Müller cells. A1 Immunofluorescence images show cells with red autofluorescence in rat retinal vertical slices acquired from TdTomato transgenic mouse retina (Tomato). A2 Immunofluorescence images show glutamine synthetase (GS) protein staining (green) in the slices depicted in A1 . A3 Immunofluorescence images show DAPI (blue) staining in the slices depicted in A1 . A4 shows merged images. Scale bar: 50 µm. B Representative trace recorded from a Müller cell identified by spontaneous red fluorescence, showing that perfusion with GSK101 (10 μM) caused significant depolarization of Müller membrane potential. C Bar chart showing GSK101-induced depolarization of Müller cell membrane potential. n = 4. * p

    Article Snippet: As negative controls, TRPV4 and GFAP antibodies were pre-adsorbed with TRPV4 blocking peptide (Alomone Labs) and GFAP blocking peptide (Bioss), respectively.

    Techniques: Functional Assay, Immunofluorescence, Transgenic Assay, Staining, Fluorescence

    TRPV4 activation enhances TNF-α production in retinal tissues. A Cumulative changes in TNF-α mRNA levels in saline-injected retinas (control) and retinas with GSK101 injection at 1 week. n = 5, * p

    Journal: Journal of Neuroinflammation

    Article Title: TRPV4-induced Müller cell gliosis and TNF-α elevation-mediated retinal ganglion cell apoptosis in glaucomatous rats via JAK2/STAT3/NF-κB pathway

    doi: 10.1186/s12974-021-02315-8

    Figure Lengend Snippet: TRPV4 activation enhances TNF-α production in retinal tissues. A Cumulative changes in TNF-α mRNA levels in saline-injected retinas (control) and retinas with GSK101 injection at 1 week. n = 5, * p

    Article Snippet: As negative controls, TRPV4 and GFAP antibodies were pre-adsorbed with TRPV4 blocking peptide (Alomone Labs) and GFAP blocking peptide (Bioss), respectively.

    Techniques: Activation Assay, Injection

    TRPV4 activation enhances the expression of GFAP. A Immunofluorescence images show GFAP protein expression profiles in rat retinal vertical slices acquired from sham-operated retinas (saline-injected; control) ( A1 ), 1 µM GSK101-injected retinas ( A2 ), and 10 µM GSK101-injected retinas ( A3 ). Retinas that received no GFAP antibody served as negative controls ( A4 ). Double immunofluorescence staining showing GFAP expression when the GFAP antibody was pre-adsorbed with its blocking peptide (BP) ( A5 ). Scale bar: 20 µm. B Representative immunoblots showing changes in GFAP protein levels in control and 10 µM GSK101-injected retinas. C Bar chart summarizing mean expression levels of GFAP under different conditions. n = 6. * p

    Journal: Journal of Neuroinflammation

    Article Title: TRPV4-induced Müller cell gliosis and TNF-α elevation-mediated retinal ganglion cell apoptosis in glaucomatous rats via JAK2/STAT3/NF-κB pathway

    doi: 10.1186/s12974-021-02315-8

    Figure Lengend Snippet: TRPV4 activation enhances the expression of GFAP. A Immunofluorescence images show GFAP protein expression profiles in rat retinal vertical slices acquired from sham-operated retinas (saline-injected; control) ( A1 ), 1 µM GSK101-injected retinas ( A2 ), and 10 µM GSK101-injected retinas ( A3 ). Retinas that received no GFAP antibody served as negative controls ( A4 ). Double immunofluorescence staining showing GFAP expression when the GFAP antibody was pre-adsorbed with its blocking peptide (BP) ( A5 ). Scale bar: 20 µm. B Representative immunoblots showing changes in GFAP protein levels in control and 10 µM GSK101-injected retinas. C Bar chart summarizing mean expression levels of GFAP under different conditions. n = 6. * p

    Article Snippet: As negative controls, TRPV4 and GFAP antibodies were pre-adsorbed with TRPV4 blocking peptide (Alomone Labs) and GFAP blocking peptide (Bioss), respectively.

    Techniques: Activation Assay, Expressing, Immunofluorescence, Injection, Double Immunofluorescence Staining, Blocking Assay, Western Blot

    TNF-α inhibition reduces TRPV4-mediated retinal cell apoptosis. A1 – A2 DAPI staining in GSK101-injected ( A1 ) and R7050 with GSK101-injected (R7050 + GSK101) ( A2 ) whole flat-mounted retinas at 7 days after injections in the regions at angle 0°. A3 – A4 Counterstained images with TUNEL staining detection of cell apoptosis (green). A5 – A6 Merged images of corresponding TUNEL and DAPI images. Scale bar, 50 µm (for all images). B Bar chart summarizing mean numbers of TUNEL-positive cells in each retina under different conditions. R7050 (1 µM, 2 μl) was pre-injected 1 day before the GSK101 injection. n = 5. ** p

    Journal: Journal of Neuroinflammation

    Article Title: TRPV4-induced Müller cell gliosis and TNF-α elevation-mediated retinal ganglion cell apoptosis in glaucomatous rats via JAK2/STAT3/NF-κB pathway

    doi: 10.1186/s12974-021-02315-8

    Figure Lengend Snippet: TNF-α inhibition reduces TRPV4-mediated retinal cell apoptosis. A1 – A2 DAPI staining in GSK101-injected ( A1 ) and R7050 with GSK101-injected (R7050 + GSK101) ( A2 ) whole flat-mounted retinas at 7 days after injections in the regions at angle 0°. A3 – A4 Counterstained images with TUNEL staining detection of cell apoptosis (green). A5 – A6 Merged images of corresponding TUNEL and DAPI images. Scale bar, 50 µm (for all images). B Bar chart summarizing mean numbers of TUNEL-positive cells in each retina under different conditions. R7050 (1 µM, 2 μl) was pre-injected 1 day before the GSK101 injection. n = 5. ** p

    Article Snippet: As negative controls, TRPV4 and GFAP antibodies were pre-adsorbed with TRPV4 blocking peptide (Alomone Labs) and GFAP blocking peptide (Bioss), respectively.

    Techniques: Inhibition, Staining, Injection, TUNEL Assay

    TRPV4 activation enhances TNF-α production in cultured Müller cells. A Morphology of cultured Müller cells. Scale bar: 50 µm. B GSK101 treatment enhanced GFAP protein levels in cultured Müller cells. n = 4. ** p

    Journal: Journal of Neuroinflammation

    Article Title: TRPV4-induced Müller cell gliosis and TNF-α elevation-mediated retinal ganglion cell apoptosis in glaucomatous rats via JAK2/STAT3/NF-κB pathway

    doi: 10.1186/s12974-021-02315-8

    Figure Lengend Snippet: TRPV4 activation enhances TNF-α production in cultured Müller cells. A Morphology of cultured Müller cells. Scale bar: 50 µm. B GSK101 treatment enhanced GFAP protein levels in cultured Müller cells. n = 4. ** p

    Article Snippet: As negative controls, TRPV4 and GFAP antibodies were pre-adsorbed with TRPV4 blocking peptide (Alomone Labs) and GFAP blocking peptide (Bioss), respectively.

    Techniques: Activation Assay, Cell Culture

    Changes in TRPV4 protein levels in retinas of rats with COH. A Representative immunoblots showing changes in TRPV4 protein levels in control and COH retinal extracts at different postoperative times (G1w, G2w, and G3w). B Bar chart summarizing mean expression levels of TRPV4 at different postoperative times. Data are presented as means ± standard errors of the mean. n = 7, 4, 6, and 5, respectively. ** p

    Journal: Journal of Neuroinflammation

    Article Title: TRPV4-induced Müller cell gliosis and TNF-α elevation-mediated retinal ganglion cell apoptosis in glaucomatous rats via JAK2/STAT3/NF-κB pathway

    doi: 10.1186/s12974-021-02315-8

    Figure Lengend Snippet: Changes in TRPV4 protein levels in retinas of rats with COH. A Representative immunoblots showing changes in TRPV4 protein levels in control and COH retinal extracts at different postoperative times (G1w, G2w, and G3w). B Bar chart summarizing mean expression levels of TRPV4 at different postoperative times. Data are presented as means ± standard errors of the mean. n = 7, 4, 6, and 5, respectively. ** p

    Article Snippet: As negative controls, TRPV4 and GFAP antibodies were pre-adsorbed with TRPV4 blocking peptide (Alomone Labs) and GFAP blocking peptide (Bioss), respectively.

    Techniques: Western Blot, Expressing

    Schematic diagram showing the signaling pathway involved in TRPV4 activation-mediated TNF-α production in Müller cells and RGC apoptosis in COH retinas. NF-κB nuclear factor-kappa B, TNF-α tumor necrosis factor-α, TNFR1 TNF receptor 1

    Journal: Journal of Neuroinflammation

    Article Title: TRPV4-induced Müller cell gliosis and TNF-α elevation-mediated retinal ganglion cell apoptosis in glaucomatous rats via JAK2/STAT3/NF-κB pathway

    doi: 10.1186/s12974-021-02315-8

    Figure Lengend Snippet: Schematic diagram showing the signaling pathway involved in TRPV4 activation-mediated TNF-α production in Müller cells and RGC apoptosis in COH retinas. NF-κB nuclear factor-kappa B, TNF-α tumor necrosis factor-α, TNFR1 TNF receptor 1

    Article Snippet: As negative controls, TRPV4 and GFAP antibodies were pre-adsorbed with TRPV4 blocking peptide (Alomone Labs) and GFAP blocking peptide (Bioss), respectively.

    Techniques: Activation Assay

    Effects of GSK101 on retinal ganglion cell (RGC) apoptosis and survival. A TRPV4 activation leads to enhanced RGC apoptosis. A1 – A3 4′,6-diamidino-2-phenylindole (DAPI) staining in saline-injected (control) ( A1 ), GSK101-injected (GSK101) ( A2 ), and HC-067 with GSK101 (HC) ( A3 ) retinas at 1 week after injection in the regions at angle 0°. Images were acquired from whole flat-mounted retinal preparations. A4 – A6 , Counterstained images with TUNEL staining detection of RGC apoptosis (green). A7 – A9 , Merged images of corresponding TUNEL and DAPI images. Scale bar: 50 µm. B Bar chart summarizing mean numbers of TUNEL-positive cells in each retina under different conditions. n = 5 for all groups. C Pre-application of HC-067 reduces RGC apoptosis in COH retinas. C1 – C3 DAPI staining in sham-operated (control) ( C1 ), COH ( C2 ), and HC-067 with COH (HC + COH) ( C3 ) retinas at G2w. C4 – C6 Counterstained images with TUNEL staining detection of RGC apoptosis (green). C7 – C9 Merged images of corresponding TUNEL and DAPI images. Scale bar, 50 μm. D Bar chart summarizing mean numbers of TUNEL-positive cells in each retina under different conditions. n = 6 for all groups. * p

    Journal: Journal of Neuroinflammation

    Article Title: TRPV4-induced Müller cell gliosis and TNF-α elevation-mediated retinal ganglion cell apoptosis in glaucomatous rats via JAK2/STAT3/NF-κB pathway

    doi: 10.1186/s12974-021-02315-8

    Figure Lengend Snippet: Effects of GSK101 on retinal ganglion cell (RGC) apoptosis and survival. A TRPV4 activation leads to enhanced RGC apoptosis. A1 – A3 4′,6-diamidino-2-phenylindole (DAPI) staining in saline-injected (control) ( A1 ), GSK101-injected (GSK101) ( A2 ), and HC-067 with GSK101 (HC) ( A3 ) retinas at 1 week after injection in the regions at angle 0°. Images were acquired from whole flat-mounted retinal preparations. A4 – A6 , Counterstained images with TUNEL staining detection of RGC apoptosis (green). A7 – A9 , Merged images of corresponding TUNEL and DAPI images. Scale bar: 50 µm. B Bar chart summarizing mean numbers of TUNEL-positive cells in each retina under different conditions. n = 5 for all groups. C Pre-application of HC-067 reduces RGC apoptosis in COH retinas. C1 – C3 DAPI staining in sham-operated (control) ( C1 ), COH ( C2 ), and HC-067 with COH (HC + COH) ( C3 ) retinas at G2w. C4 – C6 Counterstained images with TUNEL staining detection of RGC apoptosis (green). C7 – C9 Merged images of corresponding TUNEL and DAPI images. Scale bar, 50 μm. D Bar chart summarizing mean numbers of TUNEL-positive cells in each retina under different conditions. n = 6 for all groups. * p

    Article Snippet: As negative controls, TRPV4 and GFAP antibodies were pre-adsorbed with TRPV4 blocking peptide (Alomone Labs) and GFAP blocking peptide (Bioss), respectively.

    Techniques: Activation Assay, Staining, Injection, TUNEL Assay