BAA-2237 Search Results


x003899  (ATCC)
94
ATCC x003899
a) We conducted a serial dilution experiment in which we mixed two Mtb DNA standards representing lineage 4.1.2.1 (strain <t>X003899)</t> and 4.9 (strain H37ra), such that the minority strain represented 0-100% of the total DNA and then diluted Mtb DNA in human DNA, to simulate clinical samples which are overwhelmingly human DNA. We approximated the proportion of Mtb DNA found in sputum with smear microscopy grades 1+, 2+, and 3+. 7 We used hybrid capture to enrich for Mtb DNA, conducted whole genome sequencing of captured libraries on an Illumina platform. b) We additionally prospectively sampled matched Mtb diagnostic cultures and unprocessed sputum submitted to ARUP diagnostic laboratories. We performed hybrid capture and Illumina whole genome sequencing on matched samples.
X003899, supplied by ATCC, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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x003899 - by Bioz Stars, 2026-02
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mycobacterium tuberculosis (zopf) lehmann and neumann  (ATCC)
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a) We conducted a serial dilution experiment in which we mixed two Mtb DNA standards representing lineage 4.1.2.1 (strain X003899) and 4.9 (strain H37ra), such that the minority strain represented 0-100% of the total DNA and then diluted Mtb DNA in human DNA, to simulate clinical samples which are overwhelmingly human DNA. We approximated the proportion of Mtb DNA found in sputum with smear microscopy grades 1+, 2+, and 3+. 7 We used hybrid capture to enrich for Mtb DNA, conducted whole genome sequencing of captured libraries on an Illumina platform. b) We additionally prospectively sampled matched Mtb diagnostic cultures and unprocessed sputum submitted to ARUP diagnostic laboratories. We performed hybrid capture and Illumina whole genome sequencing on matched samples.

Journal: medRxiv

Article Title: Optimizing culture-free approaches to recover high quality M. tuberculosis genomic variation

doi: 10.64898/2025.12.16.25342406

Figure Lengend Snippet: a) We conducted a serial dilution experiment in which we mixed two Mtb DNA standards representing lineage 4.1.2.1 (strain X003899) and 4.9 (strain H37ra), such that the minority strain represented 0-100% of the total DNA and then diluted Mtb DNA in human DNA, to simulate clinical samples which are overwhelmingly human DNA. We approximated the proportion of Mtb DNA found in sputum with smear microscopy grades 1+, 2+, and 3+. 7 We used hybrid capture to enrich for Mtb DNA, conducted whole genome sequencing of captured libraries on an Illumina platform. b) We additionally prospectively sampled matched Mtb diagnostic cultures and unprocessed sputum submitted to ARUP diagnostic laboratories. We performed hybrid capture and Illumina whole genome sequencing on matched samples.

Article Snippet: To measure the efficiency of hybrid capture and accuracy of variant identification in captured samples, we created mixtures of reference strains H37Ra (ATCC 25177) and X003899 (ATCC BAA-2237D-2) with well characterized genomes (representing Mtb lineages 4 and 2), in which the minor strain comprised 0, 1, 2, 5, 10, 20, and 50% of the input DNA ( ).

Techniques: Serial Dilution, Microscopy, Sequencing, Diagnostic Assay