BAA-1742 Search Results


c pyruviciproducens  (ATCC)
95
ATCC c pyruviciproducens
Figure 1. Corynebacterium <t>pyruviciproducens-peptidoglycan</t> moderates the increased MyD88 expression triggered by MRSA in primary peritoneal macrophages. Primary peritoneal macrophages obtained from BALB/c mice were stimulated with CP-PGN alone (50 μg/mL, 24h), inactivated MRSA alone (100 μg/mL, 24h), or MRSA followed by CP-PGN. MyD88 protein was detected by western blot analysis (a–b). TNF-α(c) and IL-10(d) in the supernatant were detected by ELISA. All data are expressed as mean ± s.d. in triplicate determinations. Statistical evaluation was performed by one-sided, two-sample with equal variance t-tests. *p < 0.05, **p < 0.01, ***p < 0.001.
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Figure 1. Corynebacterium pyruviciproducens-peptidoglycan moderates the increased MyD88 expression triggered by MRSA in primary peritoneal macrophages. Primary peritoneal macrophages obtained from BALB/c mice were stimulated with CP-PGN alone (50 μg/mL, 24h), inactivated MRSA alone (100 μg/mL, 24h), or MRSA followed by CP-PGN. MyD88 protein was detected by western blot analysis (a–b). TNF-α(c) and IL-10(d) in the supernatant were detected by ELISA. All data are expressed as mean ± s.d. in triplicate determinations. Statistical evaluation was performed by one-sided, two-sample with equal variance t-tests. *p < 0.05, **p < 0.01, ***p < 0.001.

Journal: European Journal of Inflammation

Article Title: Corynebacterium pyruviciproducens-peptidoglycan: A novel bacterial peptidoglycan inhibiting overexpression of MyD88 in macrophages

doi: 10.1177/1721727x221095378

Figure Lengend Snippet: Figure 1. Corynebacterium pyruviciproducens-peptidoglycan moderates the increased MyD88 expression triggered by MRSA in primary peritoneal macrophages. Primary peritoneal macrophages obtained from BALB/c mice were stimulated with CP-PGN alone (50 μg/mL, 24h), inactivated MRSA alone (100 μg/mL, 24h), or MRSA followed by CP-PGN. MyD88 protein was detected by western blot analysis (a–b). TNF-α(c) and IL-10(d) in the supernatant were detected by ELISA. All data are expressed as mean ± s.d. in triplicate determinations. Statistical evaluation was performed by one-sided, two-sample with equal variance t-tests. *p < 0.05, **p < 0.01, ***p < 0.001.

Article Snippet: C. pyruviciproducens (CCUG 57046, ATCC BAA1742 obtained from Olive View Medicine Center, University of California, Los Angeles, CA, USA) and methicillin-resistant Staphylococcus aureus (MRSA) were cultured and collected to be inactivated by heat.

Techniques: Expressing, Western Blot, Enzyme-linked Immunosorbent Assay

Figure 2. Corynebacterium pyruviciproducens-peptidoglycan inhibits the increased MyD88 expression by MRSA in primary peritoneal macrophages, instead of Pam3CSK4 of a common TLR2 agonist ligand. (a) Pam3CSK4 (10 μg/mL) or CP-PGN (50 μg/mL) alone treated primary peritoneal macrophages for 24h, detecting MyD88 by western blot. (b) Primary peritoneal macrophages were stimulated by 100 μg/mL whole MRSA cells for 24 h, followed by Pam3CSK4 (10 μg/mL) or CP-PGN (50 μg/mL) for another 24h, then MyD88 detected by western blot. Western blot shown is a representative image of triplicate experiment.

Journal: European Journal of Inflammation

Article Title: Corynebacterium pyruviciproducens-peptidoglycan: A novel bacterial peptidoglycan inhibiting overexpression of MyD88 in macrophages

doi: 10.1177/1721727x221095378

Figure Lengend Snippet: Figure 2. Corynebacterium pyruviciproducens-peptidoglycan inhibits the increased MyD88 expression by MRSA in primary peritoneal macrophages, instead of Pam3CSK4 of a common TLR2 agonist ligand. (a) Pam3CSK4 (10 μg/mL) or CP-PGN (50 μg/mL) alone treated primary peritoneal macrophages for 24h, detecting MyD88 by western blot. (b) Primary peritoneal macrophages were stimulated by 100 μg/mL whole MRSA cells for 24 h, followed by Pam3CSK4 (10 μg/mL) or CP-PGN (50 μg/mL) for another 24h, then MyD88 detected by western blot. Western blot shown is a representative image of triplicate experiment.

Article Snippet: C. pyruviciproducens (CCUG 57046, ATCC BAA1742 obtained from Olive View Medicine Center, University of California, Los Angeles, CA, USA) and methicillin-resistant Staphylococcus aureus (MRSA) were cultured and collected to be inactivated by heat.

Techniques: Expressing, Western Blot

Figure 4. Corynebacterium pyruviciproducens-peptidoglycan, not the peptidoglycan of MRSA (M-PGN), reverses the increased MyD88 expression by MRSA in RAW264.7. MyD88 protein of the stimulated RAW264.7 cells was detected by western blot analysis. (a) CP-PGN (50 μg/mL) or M-PGN (50 μg/mL) alone stimulated RAW264.7 cells for 24h or 48h. (b) After MRSA (100 μg/ mL) pretreated RAW264.7 cells for 24h, the following CP-PGN (50 μg/mL) or M-PGN (50 μg/mL), respectively, stimulated cells for another 24h. Western blot shown is a representative image of triplicate experiments.

Journal: European Journal of Inflammation

Article Title: Corynebacterium pyruviciproducens-peptidoglycan: A novel bacterial peptidoglycan inhibiting overexpression of MyD88 in macrophages

doi: 10.1177/1721727x221095378

Figure Lengend Snippet: Figure 4. Corynebacterium pyruviciproducens-peptidoglycan, not the peptidoglycan of MRSA (M-PGN), reverses the increased MyD88 expression by MRSA in RAW264.7. MyD88 protein of the stimulated RAW264.7 cells was detected by western blot analysis. (a) CP-PGN (50 μg/mL) or M-PGN (50 μg/mL) alone stimulated RAW264.7 cells for 24h or 48h. (b) After MRSA (100 μg/ mL) pretreated RAW264.7 cells for 24h, the following CP-PGN (50 μg/mL) or M-PGN (50 μg/mL), respectively, stimulated cells for another 24h. Western blot shown is a representative image of triplicate experiments.

Article Snippet: C. pyruviciproducens (CCUG 57046, ATCC BAA1742 obtained from Olive View Medicine Center, University of California, Los Angeles, CA, USA) and methicillin-resistant Staphylococcus aureus (MRSA) were cultured and collected to be inactivated by heat.

Techniques: Expressing, Western Blot

Figure 3. Corynebacterium pyruviciproducens-peptidoglycan inhibits the high expression of MyD88 in RAW264.7 cell line in a dose- and time-dependent manner. MyD88 protein of RAW264.7 cells was detected by western blot analysis. (a) 50 μg/mL CP- PGN stimulated RAW264.7 cells for a prolonged time (8, 24, and 36 h). (b) Different doses of CP-PGN (10, 50, and 100 μg/mL) stimulated RAW264.7 cells for 24 h. (c) Only CP-PGN (50 μg/mL for 24h) significantly suppressed the MyD88 protein, compared with MRSA and Pam3CSK4. Western blot shown is a representative image of triplicate experiments.

Journal: European Journal of Inflammation

Article Title: Corynebacterium pyruviciproducens-peptidoglycan: A novel bacterial peptidoglycan inhibiting overexpression of MyD88 in macrophages

doi: 10.1177/1721727x221095378

Figure Lengend Snippet: Figure 3. Corynebacterium pyruviciproducens-peptidoglycan inhibits the high expression of MyD88 in RAW264.7 cell line in a dose- and time-dependent manner. MyD88 protein of RAW264.7 cells was detected by western blot analysis. (a) 50 μg/mL CP- PGN stimulated RAW264.7 cells for a prolonged time (8, 24, and 36 h). (b) Different doses of CP-PGN (10, 50, and 100 μg/mL) stimulated RAW264.7 cells for 24 h. (c) Only CP-PGN (50 μg/mL for 24h) significantly suppressed the MyD88 protein, compared with MRSA and Pam3CSK4. Western blot shown is a representative image of triplicate experiments.

Article Snippet: C. pyruviciproducens (CCUG 57046, ATCC BAA1742 obtained from Olive View Medicine Center, University of California, Los Angeles, CA, USA) and methicillin-resistant Staphylococcus aureus (MRSA) were cultured and collected to be inactivated by heat.

Techniques: Expressing, Western Blot