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  • 94
    ATCC thermus thermophilus hb27
    Thermus Thermophilus Hb27, supplied by ATCC, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/thermus thermophilus hb27/product/ATCC
    Average 94 stars, based on 1 article reviews
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    94
    ATCC t thermophilus hb27
    Size exclusion chromatography of T. <t>thermophilus</t> wild-type Soj and Soj D44A in the presence and absence of ATP. The elution volume of each species is indicated in millilitres above the respective trace. (A) In the absence of ATP, wild-type Soj elutes as a monomeric species (black). In the presence of ATP, Soj elutes as a dimer–monomer equilibrium (green). (B) Soj D44A, deficient in nucleotide hydrolysis, also elutes as a monomer in the absence of ATP (black), but elutes almost solely as a dimer in the presence of ATP (green). Red: absorbance at 260 nm indicating the elution volume of ATP and the presence of ATP in the dimeric protein.
    T Thermophilus Hb27, supplied by ATCC, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/t thermophilus hb27/product/ATCC
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    t thermophilus hb27 - by Bioz Stars, 2023-01
    94/100 stars
      Buy from Supplier

    86
    ATCC s thermophilus nvbs
    Size exclusion chromatography of T. <t>thermophilus</t> wild-type Soj and Soj D44A in the presence and absence of ATP. The elution volume of each species is indicated in millilitres above the respective trace. (A) In the absence of ATP, wild-type Soj elutes as a monomeric species (black). In the presence of ATP, Soj elutes as a dimer–monomer equilibrium (green). (B) Soj D44A, deficient in nucleotide hydrolysis, also elutes as a monomer in the absence of ATP (black), but elutes almost solely as a dimer in the presence of ATP (green). Red: absorbance at 260 nm indicating the elution volume of ATP and the presence of ATP in the dimeric protein.
    S Thermophilus Nvbs, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/s thermophilus nvbs/product/ATCC
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    s thermophilus nvbs - by Bioz Stars, 2023-01
    86/100 stars
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    Image Search Results


    Size exclusion chromatography of T. thermophilus wild-type Soj and Soj D44A in the presence and absence of ATP. The elution volume of each species is indicated in millilitres above the respective trace. (A) In the absence of ATP, wild-type Soj elutes as a monomeric species (black). In the presence of ATP, Soj elutes as a dimer–monomer equilibrium (green). (B) Soj D44A, deficient in nucleotide hydrolysis, also elutes as a monomer in the absence of ATP (black), but elutes almost solely as a dimer in the presence of ATP (green). Red: absorbance at 260 nm indicating the elution volume of ATP and the presence of ATP in the dimeric protein.

    Journal:

    Article Title: Bacterial chromosome segregation: structure and DNA binding of the Soj dimer -- a conserved biological switch

    doi: 10.1038/sj.emboj.7600530

    Figure Lengend Snippet: Size exclusion chromatography of T. thermophilus wild-type Soj and Soj D44A in the presence and absence of ATP. The elution volume of each species is indicated in millilitres above the respective trace. (A) In the absence of ATP, wild-type Soj elutes as a monomeric species (black). In the presence of ATP, Soj elutes as a dimer–monomer equilibrium (green). (B) Soj D44A, deficient in nucleotide hydrolysis, also elutes as a monomer in the absence of ATP (black), but elutes almost solely as a dimer in the presence of ATP (green). Red: absorbance at 260 nm indicating the elution volume of ATP and the presence of ATP in the dimeric protein.

    Article Snippet: Soj from T. thermophilus HB27 (ATCC BAA-163D) was cloned into pHis17 to generate a C-terminally hexa-histidine-tagged protein.

    Techniques: Size-exclusion Chromatography

    Sequence alignment of putative activating peptides of Spo0J, MinE and FtsA. Alignment of the N-terminal regions of Spo0J and MinE proteins with the C-terminus of FtsA proteins reveals a remarkable sequence homology, which includes a universally conserved basic (lys/arg) residue (arginine 10 in T. thermophilus Spo0J). The functional homology of these putative activating peptides is further strengthened by the observations that E. coli FtsA C-terminus can stimulate Soj and mutation of the arginine 10 to alanine in the Spo0JN20 peptide abrogates stimulation of ATP hydrolysis (Figure 7).

    Journal:

    Article Title: Bacterial chromosome segregation: structure and DNA binding of the Soj dimer -- a conserved biological switch

    doi: 10.1038/sj.emboj.7600530

    Figure Lengend Snippet: Sequence alignment of putative activating peptides of Spo0J, MinE and FtsA. Alignment of the N-terminal regions of Spo0J and MinE proteins with the C-terminus of FtsA proteins reveals a remarkable sequence homology, which includes a universally conserved basic (lys/arg) residue (arginine 10 in T. thermophilus Spo0J). The functional homology of these putative activating peptides is further strengthened by the observations that E. coli FtsA C-terminus can stimulate Soj and mutation of the arginine 10 to alanine in the Spo0JN20 peptide abrogates stimulation of ATP hydrolysis (Figure 7).

    Article Snippet: Soj from T. thermophilus HB27 (ATCC BAA-163D) was cloned into pHis17 to generate a C-terminally hexa-histidine-tagged protein.

    Techniques: Sequencing, Functional Assay, Mutagenesis