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    Alomone Labs anti asic3 antibody
    Amiloride and ibuprofen treatment decreased ASIC expression Nucleus pulposus cells from normal and degenerated tissue were treated with amiloride (1 mM) or ibuprofen (100 μM) for 24 h and ASIC1 (A, C) and <t>ASIC3</t> (B, D) expression was determined
    Anti Asic3 Antibody, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Amiloride and ibuprofen treatment decreased ASIC expression Nucleus pulposus cells from normal and degenerated tissue were treated with amiloride (1 mM) or ibuprofen (100 μM) for 24 h and ASIC1 (A, C) and ASIC3 (B, D) expression was determined

    Journal: Acta Biochimica et Biophysica Sinica

    Article Title: Expression of acid-sensing ion channels in nucleus pulposus cells of the human intervertebral disk is regulated by non-steroid anti-inflammatory drugs

    doi: 10.1093/abbs/gmu067

    Figure Lengend Snippet: Amiloride and ibuprofen treatment decreased ASIC expression Nucleus pulposus cells from normal and degenerated tissue were treated with amiloride (1 mM) or ibuprofen (100 μM) for 24 h and ASIC1 (A, C) and ASIC3 (B, D) expression was determined

    Article Snippet: Primary antibodies against ASIC subunits were used as follows: anti-ASIC1 (E-15, sc-13903, 1 : 1000; Santa Cruz, Santa Cruz, USA); anti-ASIC2 (ASC-012, 1 : 1000; Alomone Labs, Jerusalem, Israel); anti-ASIC3 (ASC-018, 1 : 1500; Alomone Labs); and anti-ASIC4 (N-20, sc-22324, 1 : 1000; Santa Cruz).

    Techniques: Expressing

    Detection of ASICs transcripts and expression in nucleus pulposus cells of human intervertebral disc cells (A) Expression of mRNA of ASIC1a, ASIC1b, and ASIC3 subunits in nucleus pulposus cells of the human intervertebral disc cells was determined

    Journal: Acta Biochimica et Biophysica Sinica

    Article Title: Expression of acid-sensing ion channels in nucleus pulposus cells of the human intervertebral disk is regulated by non-steroid anti-inflammatory drugs

    doi: 10.1093/abbs/gmu067

    Figure Lengend Snippet: Detection of ASICs transcripts and expression in nucleus pulposus cells of human intervertebral disc cells (A) Expression of mRNA of ASIC1a, ASIC1b, and ASIC3 subunits in nucleus pulposus cells of the human intervertebral disc cells was determined

    Article Snippet: Primary antibodies against ASIC subunits were used as follows: anti-ASIC1 (E-15, sc-13903, 1 : 1000; Santa Cruz, Santa Cruz, USA); anti-ASIC2 (ASC-012, 1 : 1000; Alomone Labs, Jerusalem, Israel); anti-ASIC3 (ASC-018, 1 : 1500; Alomone Labs); and anti-ASIC4 (N-20, sc-22324, 1 : 1000; Santa Cruz).

    Techniques: Expressing

    Effect of ASA on ASIC3 expression in DRG. (A–F) Fluorescence microscope image of FB- (A, D), ASIC3- (B, E) labeled DRG neurons and their overlay (C, F) 3 weeks after MIA injection (1 week after FB injection). Arrowheads (white) indicate ASIC3 positive cells in FB-labeled neurons and yellow arrows indicate ASIC3 negative cells in FB-labeled neurons innervating the knee joint. Scale bar: 100 μm. (G) Effect of ASA on ASIC3 expression in DRG. ASIC3 expression was counted in FB-labeled neurons in L3-4 DRGs and quantified as the percentage of total FB-labeled neurons. Means ± S.E.M, N = 4–6, ∗: p

    Journal: Heliyon

    Article Title: Mechanism of aspirin-induced inhibition on the secondary hyperalgesia in osteoarthritis model rats

    doi: 10.1016/j.heliyon.2020.e03963

    Figure Lengend Snippet: Effect of ASA on ASIC3 expression in DRG. (A–F) Fluorescence microscope image of FB- (A, D), ASIC3- (B, E) labeled DRG neurons and their overlay (C, F) 3 weeks after MIA injection (1 week after FB injection). Arrowheads (white) indicate ASIC3 positive cells in FB-labeled neurons and yellow arrows indicate ASIC3 negative cells in FB-labeled neurons innervating the knee joint. Scale bar: 100 μm. (G) Effect of ASA on ASIC3 expression in DRG. ASIC3 expression was counted in FB-labeled neurons in L3-4 DRGs and quantified as the percentage of total FB-labeled neurons. Means ± S.E.M, N = 4–6, ∗: p

    Article Snippet: After washing with 0.1 M PBS (pH 7.4), sections were incubated at 4 °C overnight with rabbit anti-ASIC3 antibody (1:500, Alomone Labs, Jerusalem, Israel).

    Techniques: Expressing, Fluorescence, Microscopy, Labeling, Injection

    ASIC3 was increased in lumbar DRGs . ASIC3 was increased in lumbar DRGs in intraplantar carrageenan-induced hyperalgesia and further attenuated by EA at ST36 acupoint in mice. (a) ASIC3 immunoreactive neurons were found in lumbar DRGs in ipsilateral site of saline-injected group. (b) ASIC3 immunoreactive neurons were found in contralateral site of saline-injected group. (c) ASIC3 immunoreactive neurons were increased in ipsilateral site of carrageenan-induced inflammation. (d) ASIC3 immunoreactive neurons were not increased in contralateral site of carrageenan-induced inflammation. (e) Carrageenan-induced increase of ASIC3 was attenuated by EA in ipsilateral site of inflammation. (f) ASIC3 immunoreactive neurons were not altered by EA in contralateral site of inflammation. Arrows indicated immunoreactive neurons of ASIC3.

    Journal: Journal of Biomedical Science

    Article Title: Acid-sensing ion channel 3 mediates peripheral anti-hyperalgesia effects of acupuncture in mice inflammatory pain

    doi: 10.1186/1423-0127-18-82

    Figure Lengend Snippet: ASIC3 was increased in lumbar DRGs . ASIC3 was increased in lumbar DRGs in intraplantar carrageenan-induced hyperalgesia and further attenuated by EA at ST36 acupoint in mice. (a) ASIC3 immunoreactive neurons were found in lumbar DRGs in ipsilateral site of saline-injected group. (b) ASIC3 immunoreactive neurons were found in contralateral site of saline-injected group. (c) ASIC3 immunoreactive neurons were increased in ipsilateral site of carrageenan-induced inflammation. (d) ASIC3 immunoreactive neurons were not increased in contralateral site of carrageenan-induced inflammation. (e) Carrageenan-induced increase of ASIC3 was attenuated by EA in ipsilateral site of inflammation. (f) ASIC3 immunoreactive neurons were not altered by EA in contralateral site of inflammation. Arrows indicated immunoreactive neurons of ASIC3.

    Article Snippet: After blocking, slides were incubated overnight with primary antibodies prepared in a blocking solution at 4°C (rabbit anti-ASIC3, 1:1000, Alomone Lab, Jerusalem, Israel).

    Techniques: Mouse Assay, Injection

    Comparative quantitative Real-time PCR analysis for ASIC3 mRNAs from control . Comparative quantitative Real-time PCR analysis for ASIC3 mRNAs from control, inflamed and EA groups. ASIC3 mRNA was reliably increased in lumbar DRGs in both carrageenan- and CFA-induced inflammation pain and further reduced by EA at ST36 acupoint in mice. There is an only unilateral increase in ASIC3 mRNA 24 hours after carrageenan or CFA-induced inflammation. **Significantly greater than control mice (p

    Journal: Journal of Biomedical Science

    Article Title: Acid-sensing ion channel 3 mediates peripheral anti-hyperalgesia effects of acupuncture in mice inflammatory pain

    doi: 10.1186/1423-0127-18-82

    Figure Lengend Snippet: Comparative quantitative Real-time PCR analysis for ASIC3 mRNAs from control . Comparative quantitative Real-time PCR analysis for ASIC3 mRNAs from control, inflamed and EA groups. ASIC3 mRNA was reliably increased in lumbar DRGs in both carrageenan- and CFA-induced inflammation pain and further reduced by EA at ST36 acupoint in mice. There is an only unilateral increase in ASIC3 mRNA 24 hours after carrageenan or CFA-induced inflammation. **Significantly greater than control mice (p

    Article Snippet: After blocking, slides were incubated overnight with primary antibodies prepared in a blocking solution at 4°C (rabbit anti-ASIC3, 1:1000, Alomone Lab, Jerusalem, Israel).

    Techniques: Real-time Polymerase Chain Reaction, Mouse Assay

    ASIC3 was increased in lumbar DRGs in intraplantar CFA-induced hyperalgesia . ASIC3 was increased in lumbar DRGs in intraplantar CFA-induced hyperalgesia and decreased by EA at ST36 acupoint in mice. (a), (b) ASIC3 immunoreactive neurons were found in lumbar DRGs in both ipsilateral and contralateral site of saline-injected group. (c) ASIC3 immunoreactive neurons were increased in ipsilateral site of CFA-induced inflammation. (d) ASIC3 immunoreactive neurons were not increased in contralateral site of CFA-induced inflammation. (e) CFA-induced increase of ASIC3 was attenuated by EA in ipsilateral site of inflammation. (f) ASIC3 immunoreactive neurons were not altered by EA in contralateral site of inflammation. Arrows indicated immunoreactive neurons of ASIC3.

    Journal: Journal of Biomedical Science

    Article Title: Acid-sensing ion channel 3 mediates peripheral anti-hyperalgesia effects of acupuncture in mice inflammatory pain

    doi: 10.1186/1423-0127-18-82

    Figure Lengend Snippet: ASIC3 was increased in lumbar DRGs in intraplantar CFA-induced hyperalgesia . ASIC3 was increased in lumbar DRGs in intraplantar CFA-induced hyperalgesia and decreased by EA at ST36 acupoint in mice. (a), (b) ASIC3 immunoreactive neurons were found in lumbar DRGs in both ipsilateral and contralateral site of saline-injected group. (c) ASIC3 immunoreactive neurons were increased in ipsilateral site of CFA-induced inflammation. (d) ASIC3 immunoreactive neurons were not increased in contralateral site of CFA-induced inflammation. (e) CFA-induced increase of ASIC3 was attenuated by EA in ipsilateral site of inflammation. (f) ASIC3 immunoreactive neurons were not altered by EA in contralateral site of inflammation. Arrows indicated immunoreactive neurons of ASIC3.

    Article Snippet: After blocking, slides were incubated overnight with primary antibodies prepared in a blocking solution at 4°C (rabbit anti-ASIC3, 1:1000, Alomone Lab, Jerusalem, Israel).

    Techniques: Mouse Assay, Injection

    ASIC3 was increased in lumbar DRGs in both intraplantar carrageenan- and CFA-induced hyperalgesia . ASIC3 was increased in lumbar DRGs in both intraplantar carrageenan- and CFA-induced hyperalgesia and further attenuated by EA at ST36 acupoint in mice. (a) ASIC3 proteins were increased in ipsilateral site of carrageenan-mediated inflammation and ameliorated by EA at ST36 acupoint (b) ASIC3 proteins were not changed in contralateral site of carrageenan-mediated inflammation and EA stimulation. (c) ASIC3 proteins were increased in ipsilateral site of CFA-induced inflammation and attenuated by EA at ST36 acupoint (d) ASIC3 proteins were not altered in contralateral site of CFA-mediated inflammation and EA stimulation.

    Journal: Journal of Biomedical Science

    Article Title: Acid-sensing ion channel 3 mediates peripheral anti-hyperalgesia effects of acupuncture in mice inflammatory pain

    doi: 10.1186/1423-0127-18-82

    Figure Lengend Snippet: ASIC3 was increased in lumbar DRGs in both intraplantar carrageenan- and CFA-induced hyperalgesia . ASIC3 was increased in lumbar DRGs in both intraplantar carrageenan- and CFA-induced hyperalgesia and further attenuated by EA at ST36 acupoint in mice. (a) ASIC3 proteins were increased in ipsilateral site of carrageenan-mediated inflammation and ameliorated by EA at ST36 acupoint (b) ASIC3 proteins were not changed in contralateral site of carrageenan-mediated inflammation and EA stimulation. (c) ASIC3 proteins were increased in ipsilateral site of CFA-induced inflammation and attenuated by EA at ST36 acupoint (d) ASIC3 proteins were not altered in contralateral site of CFA-mediated inflammation and EA stimulation.

    Article Snippet: After blocking, slides were incubated overnight with primary antibodies prepared in a blocking solution at 4°C (rabbit anti-ASIC3, 1:1000, Alomone Lab, Jerusalem, Israel).

    Techniques: Mouse Assay