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    Alomone Labs aqp5
    Loss of Nfib in mesenchyme affects type I and type II epithelial cell differentiation. E18.5 lung sections from Nfib flox/flox and Nfib flox/flox , D1-Cre embryos were stained for <t>AQP5</t> (A, B), pro-SPC (C, D) and CC10 (E, F). AQP5, pro-SPC and Foxj1 transcript
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    Loss of Nfib in mesenchyme affects type I and type II epithelial cell differentiation. E18.5 lung sections from Nfib flox/flox and Nfib flox/flox , D1-Cre embryos were stained for AQP5 (A, B), pro-SPC (C, D) and CC10 (E, F). AQP5, pro-SPC and Foxj1 transcript

    Journal: Developmental biology

    Article Title: Mesenchymal Nuclear factor I B regulates cell proliferation and epithelial differentiation during lung maturation

    doi: 10.1016/j.ydbio.2011.04.002

    Figure Lengend Snippet: Loss of Nfib in mesenchyme affects type I and type II epithelial cell differentiation. E18.5 lung sections from Nfib flox/flox and Nfib flox/flox , D1-Cre embryos were stained for AQP5 (A, B), pro-SPC (C, D) and CC10 (E, F). AQP5, pro-SPC and Foxj1 transcript

    Article Snippet: For immunohistochemistry, antibodies against the following proteins were used: CC10 (Santa Cruz T-18, 1:200), pro-SPC (Chemicon AB3786, 1:2000), PCNA (Santa Cruz FL-261, 1:200), phospho-histone H3 (Sigma HTA28, 1:200), cleaved Caspase-3 (Cell Signaling #9661, 1:200), Ki67 (Abcam ab15580, 1:400), TTF-1 (Dako 8G7G3/1, 1:200), Vimentin (Sigma LN-6, 1:400), Caveolin-1 (Santa Cruz N-20, 1:200), smooth muscle actin (SMA) (Sigma 1A4, 1:5000), AQP5 (Alomone labs AQP-005, 1:200) and NFI-B (Active Motif 1:1000).

    Techniques: Cell Differentiation, Staining

    Expression of known AT1 cell markers in rat microarray data. Log2 expression data were generated from microarray experiments: AT1-like cells differentiated in culture ( blue ) (Day 2-6), freshly isolated AT1 cells ( purple ), freshly isolated AT2 cells ( red ), and other tissues ( black ). Table at bottom right includes previously described AT1 cell–specific genes, their associated Illumina Probe IDs, and their FDR-corrected P values in this study. FDR adjustment is based on the number of tests shown for known genes. AGER, advanced glycosylation end product–specific receptor; AQP5, aquaporin 5; CAV, caveolin; FDR, false-discovery rate; ILMN, Illumina probe number; PDPN, podoplanin. **Indicates significantly greater in rat AT1 and AT1-like cells compared to all others.

    Journal: American Journal of Respiratory Cell and Molecular Biology

    Article Title: Cross-Species Transcriptome Profiling Identifies New Alveolar Epithelial Type I Cell–Specific Genes

    doi: 10.1165/rcmb.2016-0071OC

    Figure Lengend Snippet: Expression of known AT1 cell markers in rat microarray data. Log2 expression data were generated from microarray experiments: AT1-like cells differentiated in culture ( blue ) (Day 2-6), freshly isolated AT1 cells ( purple ), freshly isolated AT2 cells ( red ), and other tissues ( black ). Table at bottom right includes previously described AT1 cell–specific genes, their associated Illumina Probe IDs, and their FDR-corrected P values in this study. FDR adjustment is based on the number of tests shown for known genes. AGER, advanced glycosylation end product–specific receptor; AQP5, aquaporin 5; CAV, caveolin; FDR, false-discovery rate; ILMN, Illumina probe number; PDPN, podoplanin. **Indicates significantly greater in rat AT1 and AT1-like cells compared to all others.

    Article Snippet: Blots were incubated with rabbit anti–epithelial sodium channel (ENaC) γ (1:200, sc-21014; Santa Cruz Biotechnology, Santa Cruz, CA), anti-semaphorin 3B (SEMA3B) (1:500, ; Abnova, Jhongli, Taiwan), anti-SEMA3E (1:100, AP7976b; Abgent, San Diego, CA), anti-GRAMD2 (1:100, ab84567; Abcam, Cambridge, MA), anti-SFTPC (1:200, AB3786; Millipore, Billerica, MA), and anti-AQP5 (1:200, AQP-005; Alomone Labs, Jerusalem, Israel).

    Techniques: Expressing, Microarray, Generated, Isolation

    Validation of GRAMD2 expression in AT1 cells in lung tissue. ( A ) Confocal images for GRAMD2/SFTPC double staining in mouse lung sections show that GRAMD2 does not colocalize with SFTPC. DAPI is the nuclear counterstain. Scale bar : 20 µm. ( B ) Confocal images for GRAMD2/AQP5 double staining in mouse lung sections shows that GRAMD2 colocalizes with AQP5. DAPI is the nuclear counterstain. Scale bar : 20 µm. DAPI, 4’,6-diamidino-2-phenylindole.

    Journal: American Journal of Respiratory Cell and Molecular Biology

    Article Title: Cross-Species Transcriptome Profiling Identifies New Alveolar Epithelial Type I Cell–Specific Genes

    doi: 10.1165/rcmb.2016-0071OC

    Figure Lengend Snippet: Validation of GRAMD2 expression in AT1 cells in lung tissue. ( A ) Confocal images for GRAMD2/SFTPC double staining in mouse lung sections show that GRAMD2 does not colocalize with SFTPC. DAPI is the nuclear counterstain. Scale bar : 20 µm. ( B ) Confocal images for GRAMD2/AQP5 double staining in mouse lung sections shows that GRAMD2 colocalizes with AQP5. DAPI is the nuclear counterstain. Scale bar : 20 µm. DAPI, 4’,6-diamidino-2-phenylindole.

    Article Snippet: Blots were incubated with rabbit anti–epithelial sodium channel (ENaC) γ (1:200, sc-21014; Santa Cruz Biotechnology, Santa Cruz, CA), anti-semaphorin 3B (SEMA3B) (1:500, ; Abnova, Jhongli, Taiwan), anti-SEMA3E (1:100, AP7976b; Abgent, San Diego, CA), anti-GRAMD2 (1:100, ab84567; Abcam, Cambridge, MA), anti-SFTPC (1:200, AB3786; Millipore, Billerica, MA), and anti-AQP5 (1:200, AQP-005; Alomone Labs, Jerusalem, Israel).

    Techniques: Expressing, Double Staining