Article Title: Persistent inflammation induces GluR2 internalization via NMDA receptor-triggered PKC activation in dorsal horn neurons
Figure Lengend Snippet: Reduced binding affinity of GluR2 to GRIP1, but not PICK1, in dorsal horn 1 day post-CFA. (A) Left panel, co-immunoprecipitation of GluR2 and GRIP1 with anti-GRIP1; middle panel, total expression of GluR2 and GRIP1; right panel, statistical summary for the ratios of the densities from the CFA-treated groups to those from the saline-treated groups. (B) Left panel, coimmunoprecipitation of GluR2 and PICK1 with anti-PICK1; middle panel, total expression of GluR2 and PICK1; right panel, statistical summary for the ratios of the densities from the CFA-treated groups to those from the saline-treated groups. IB: immunoblotting. IP: immunoprecipitation. Sal: saline. *P < 0.05 vs the saline-treated groups. β-actin was used as a loading control.
Article Snippet: The following antibodies were used: rabbit anti-GluR2 (Chemicon, Temecula, CA), mouse anti-GluR2 (Chemicon), rabbit anti-GluR2-p Ser 880 ( Chung et al., 2003 ; Steinberg et al., 2006 ), rabbit ant-PICK1 ( Steinberg et al., 2006 ), rabbit anti-GRIP1 (Upstate, Lake Placid, NY), mouse anti-PSD-95 (Upstate), rabbit anti-PSD-93 (Alomone Labs Ltd, Jerusalem, Israel), rabbit anti-NR2B (Chemicon), rabbit anti-NR1 (Chemicon), rabbit anti-NR2A/2B (Chemicon), rabbit anti-PKCα (Santa Cruz Biotechnology, Santa Cruz, CA), rabbit anti-PKC β (Santa Cruz Biotechnology), rabbit anti-PKCγ (Santa Cruz Biotechnology), rabbit anti- N -cadherin (BD Transduction Laboratories, San Jose, CA), rabbit anti-stargazin (Upstate), mouse anti-α-adaptin (Sigma), and mouse anti-β-actin (Sigma).
Techniques: Binding Assay, Immunoprecipitation, Expressing, Western Blot