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  • 93
    Alomone Labs plexina1
    Plexina1, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/plexina1/product/Alomone Labs
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    plexina1 - by Bioz Stars, 2023-02
    93/100 stars
      Buy from Supplier

    93
    Alomone Labs anti plexin a1 extracellular
    ( A ) Western blot analysis of the expression of Sema 3 A, L1CAM and the Sema 3 A receptors Nrp1 and <t>Plexin</t> <t>A1</t> in the cortex of PND1 control and AnkB440 KO mice. ( B ) Quantification of protein levels normalized to actin in cortical lysates from PND1 mice of indicated genotypes relative to the normalized levels of each protein in control brains. Data show mean ± SEM for three biological replicates per genotype for one experiment. Unpaired t test. *p < 0.05. ( C ) Western blot analysis of total and surface levels of indicated proteins in DIV3 control and AnkB440 KO cortical neurons. ( D ) Quantification of total and surface protein levels normalized to actin relative to the normalized levels of each protein in control cortical neurons. Data show mean ± SEM for three biological replicates per genotype for one experiment. Unpaired t test. **p < 0.01. ( E ) Images show PLA signal between L1CAM and Nrp1 at the cell surface of the axon and GCs of DIV3 cortical neurons from the indicated genotypes. This assay used an Nrp1 antibody that selectively recognizes an extracellular epitope. Phalloidin staining was used to identify GCs. Scale bar, 2 μm. ( F ) Quantification of PLA signal at the GC surface relative to GC area collected from an average n = 15 GCs/genotype. The box and whisker plots represent all data points arranged from minimum to maximum. One-way ANOVA with Tukey’s post hoc analysis test for multiple comparisons. ****p < 0.0001. ( G ) Images show Nrp1 localization at the surface of GCs of DIV3 neurons untreated and treated with Sema 3 A, which induces the internalization of surface Nrp1. Scale bar, 1 μm. ( H ) Quantification of surface Nrp1 levels at GCs relative to GC area at the basal state and upon Sema 3A-induced Nrp1 internalization. Data represent mean ± SEM collected from an average of n = 90 GCs/treatment/genotype/experiment. Each dot represents one out of three independent experiments. One-way ANOVA with Tukey’s post hoc analysis test for multiple comparisons. **p < 0.01. Figure 7—source data 1. AnkB440 stabilizes the L1CAM-Nrp1 complex at the cell surface of GCs.
    Anti Plexin A1 Extracellular, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti plexin a1 extracellular/product/Alomone Labs
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti plexin a1 extracellular - by Bioz Stars, 2023-02
    93/100 stars
      Buy from Supplier

    Image Search Results


    ( A ) Western blot analysis of the expression of Sema 3 A, L1CAM and the Sema 3 A receptors Nrp1 and Plexin A1 in the cortex of PND1 control and AnkB440 KO mice. ( B ) Quantification of protein levels normalized to actin in cortical lysates from PND1 mice of indicated genotypes relative to the normalized levels of each protein in control brains. Data show mean ± SEM for three biological replicates per genotype for one experiment. Unpaired t test. *p < 0.05. ( C ) Western blot analysis of total and surface levels of indicated proteins in DIV3 control and AnkB440 KO cortical neurons. ( D ) Quantification of total and surface protein levels normalized to actin relative to the normalized levels of each protein in control cortical neurons. Data show mean ± SEM for three biological replicates per genotype for one experiment. Unpaired t test. **p < 0.01. ( E ) Images show PLA signal between L1CAM and Nrp1 at the cell surface of the axon and GCs of DIV3 cortical neurons from the indicated genotypes. This assay used an Nrp1 antibody that selectively recognizes an extracellular epitope. Phalloidin staining was used to identify GCs. Scale bar, 2 μm. ( F ) Quantification of PLA signal at the GC surface relative to GC area collected from an average n = 15 GCs/genotype. The box and whisker plots represent all data points arranged from minimum to maximum. One-way ANOVA with Tukey’s post hoc analysis test for multiple comparisons. ****p < 0.0001. ( G ) Images show Nrp1 localization at the surface of GCs of DIV3 neurons untreated and treated with Sema 3 A, which induces the internalization of surface Nrp1. Scale bar, 1 μm. ( H ) Quantification of surface Nrp1 levels at GCs relative to GC area at the basal state and upon Sema 3A-induced Nrp1 internalization. Data represent mean ± SEM collected from an average of n = 90 GCs/treatment/genotype/experiment. Each dot represents one out of three independent experiments. One-way ANOVA with Tukey’s post hoc analysis test for multiple comparisons. **p < 0.01. Figure 7—source data 1. AnkB440 stabilizes the L1CAM-Nrp1 complex at the cell surface of GCs.

    Journal: eLife

    Article Title: Giant ankyrin-B mediates transduction of axon guidance and collateral branch pruning factor sema 3A

    doi: 10.7554/eLife.69815

    Figure Lengend Snippet: ( A ) Western blot analysis of the expression of Sema 3 A, L1CAM and the Sema 3 A receptors Nrp1 and Plexin A1 in the cortex of PND1 control and AnkB440 KO mice. ( B ) Quantification of protein levels normalized to actin in cortical lysates from PND1 mice of indicated genotypes relative to the normalized levels of each protein in control brains. Data show mean ± SEM for three biological replicates per genotype for one experiment. Unpaired t test. *p < 0.05. ( C ) Western blot analysis of total and surface levels of indicated proteins in DIV3 control and AnkB440 KO cortical neurons. ( D ) Quantification of total and surface protein levels normalized to actin relative to the normalized levels of each protein in control cortical neurons. Data show mean ± SEM for three biological replicates per genotype for one experiment. Unpaired t test. **p < 0.01. ( E ) Images show PLA signal between L1CAM and Nrp1 at the cell surface of the axon and GCs of DIV3 cortical neurons from the indicated genotypes. This assay used an Nrp1 antibody that selectively recognizes an extracellular epitope. Phalloidin staining was used to identify GCs. Scale bar, 2 μm. ( F ) Quantification of PLA signal at the GC surface relative to GC area collected from an average n = 15 GCs/genotype. The box and whisker plots represent all data points arranged from minimum to maximum. One-way ANOVA with Tukey’s post hoc analysis test for multiple comparisons. ****p < 0.0001. ( G ) Images show Nrp1 localization at the surface of GCs of DIV3 neurons untreated and treated with Sema 3 A, which induces the internalization of surface Nrp1. Scale bar, 1 μm. ( H ) Quantification of surface Nrp1 levels at GCs relative to GC area at the basal state and upon Sema 3A-induced Nrp1 internalization. Data represent mean ± SEM collected from an average of n = 90 GCs/treatment/genotype/experiment. Each dot represents one out of three independent experiments. One-way ANOVA with Tukey’s post hoc analysis test for multiple comparisons. **p < 0.01. Figure 7—source data 1. AnkB440 stabilizes the L1CAM-Nrp1 complex at the cell surface of GCs.

    Article Snippet: Antibody , anti-Plexin A1 extracellular (Rabbit polyclonal) , Alomone , Cat# APR-081, RRID: AB_2756765 , WB (1:200).

    Techniques: Western Blot, Expressing, Staining, Whisker Assay

    Journal: eLife

    Article Title: Giant ankyrin-B mediates transduction of axon guidance and collateral branch pruning factor sema 3A

    doi: 10.7554/eLife.69815

    Figure Lengend Snippet:

    Article Snippet: Antibody , anti-Plexin A1 extracellular (Rabbit polyclonal) , Alomone , Cat# APR-081, RRID: AB_2756765 , WB (1:200).

    Techniques: Knock-Out, Recombinant, Mutagenesis, In Situ