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    Alomone Labs antibodies recognizing p2y 1
    The effect of long-term (96 h) serum deprivation on the relative expression levels of <t>P2Y</t> <t>1</t> , P2Y 2 and P2Y 12 receptors. Total protein isolated from glioma C6 cells was subjected to SDS-PAGE on 12% acrylamide gel and transferred onto nitrocellulose membrane. Filters were probed with the indicated P2Y antisera. Protein bands were detected with secondary antisera coupled to HRP by ECL chemiluminescences. Band intensity was determined by densitometry analysis. The data represent the means ± SD from three independent experiments
    Antibodies Recognizing P2y 1, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    The effect of long-term (96 h) serum deprivation on the relative expression levels of P2Y 1 , P2Y 2 and P2Y 12 receptors. Total protein isolated from glioma C6 cells was subjected to SDS-PAGE on 12% acrylamide gel and transferred onto nitrocellulose membrane. Filters were probed with the indicated P2Y antisera. Protein bands were detected with secondary antisera coupled to HRP by ECL chemiluminescences. Band intensity was determined by densitometry analysis. The data represent the means ± SD from three independent experiments

    Journal: Purinergic Signalling

    Article Title: P2Y 1 and P2Y 12 receptor cross-talk in calcium signalling: Evidence from nonstarved and long-term serum-deprived glioma C6 cells

    doi: 10.1007/s11302-007-9051-5

    Figure Lengend Snippet: The effect of long-term (96 h) serum deprivation on the relative expression levels of P2Y 1 , P2Y 2 and P2Y 12 receptors. Total protein isolated from glioma C6 cells was subjected to SDS-PAGE on 12% acrylamide gel and transferred onto nitrocellulose membrane. Filters were probed with the indicated P2Y antisera. Protein bands were detected with secondary antisera coupled to HRP by ECL chemiluminescences. Band intensity was determined by densitometry analysis. The data represent the means ± SD from three independent experiments

    Article Snippet: Antibodies recognizing P2Y 1 , P2Y 2 , and P2Y 12 were purchased from Alomone and Sigma Aldrich Chemical labs. Horseradish peroxidase-conjugated anti-rabbit IgG was from Cell Signalling.

    Techniques: Expressing, Isolation, SDS Page, Acrylamide Gel Assay

    The effect of AR-C69931MX, the P2Y 12 receptor antagonist, on Ca 2+ signals evoked by 30 μM 2MeSADP ( a , b ), 10 μM ADP ( c , d ), and 100 μM UTP ( e , f ). Black lines represent mean ratio values of Ca 2+ responses of cells untreated with P2Y 12 antagonist. a , c , e Nonstarved cells, respectively, n = 71, n = 51, n = 73. b , d 96-h serum-starved cells, respectively, n = 79, n = 134. Gray lines in the same panels represent mean ratio value of Ca 2+ responses of cells pre-treated for 3 min with 10 μM AR-C69931MX ( a , n = 95; b , n = 80; c , n = 19 ; d , n = 175; e , n = 79) and then, while still in its presence, stimulated by agonist, as indicated by arrows . f 100 μM UTP-evoked Ca 2+ signals in the control cells ( n = 73, black line ); and 96-h serum-starved cells, ( n = 120, gray line ). Each trace represents mean ratio value from five experiments. *** P < 0.001. Statistically insignificant differences are indicated with a minus sign

    Journal: Purinergic Signalling

    Article Title: P2Y 1 and P2Y 12 receptor cross-talk in calcium signalling: Evidence from nonstarved and long-term serum-deprived glioma C6 cells

    doi: 10.1007/s11302-007-9051-5

    Figure Lengend Snippet: The effect of AR-C69931MX, the P2Y 12 receptor antagonist, on Ca 2+ signals evoked by 30 μM 2MeSADP ( a , b ), 10 μM ADP ( c , d ), and 100 μM UTP ( e , f ). Black lines represent mean ratio values of Ca 2+ responses of cells untreated with P2Y 12 antagonist. a , c , e Nonstarved cells, respectively, n = 71, n = 51, n = 73. b , d 96-h serum-starved cells, respectively, n = 79, n = 134. Gray lines in the same panels represent mean ratio value of Ca 2+ responses of cells pre-treated for 3 min with 10 μM AR-C69931MX ( a , n = 95; b , n = 80; c , n = 19 ; d , n = 175; e , n = 79) and then, while still in its presence, stimulated by agonist, as indicated by arrows . f 100 μM UTP-evoked Ca 2+ signals in the control cells ( n = 73, black line ); and 96-h serum-starved cells, ( n = 120, gray line ). Each trace represents mean ratio value from five experiments. *** P < 0.001. Statistically insignificant differences are indicated with a minus sign

    Article Snippet: Antibodies recognizing P2Y 1 , P2Y 2 , and P2Y 12 were purchased from Alomone and Sigma Aldrich Chemical labs. Horseradish peroxidase-conjugated anti-rabbit IgG was from Cell Signalling.

    Techniques:

    The effect of AR-C69931MX, the P2Y 12 receptor antagonist, on the first and second phase of calcium response in cells grown in the presence of serum. a Cells in Ca 2+ -free buffer were stimulated by 10 μM ADP ( arrow ). Two cell groups were studied: the first experimental group was treated with 10 μM AR-C69931MX for 3 min before ADP addition and release of calcium from the ER ( light gray line , ARC - ADP), while the second experimental group was treated for 3 min with 10 μM AR-C69931MX just after the end of calcium transient caused by ADP-induced release from the ER ( dark gray line , ADP - ARC). The control cell group was induced with ADP, without use of AR-C69931MX ( black line , Ctrl). Then, in all cell groups, the medium was exchanged with fresh medium containing 2 mM CaCl 2 ( arrow ). b Strength of two phases of calcium response. Colour of bars as described above. Difference between bars marked with a minus sign is statistically insignificant

    Journal: Purinergic Signalling

    Article Title: P2Y 1 and P2Y 12 receptor cross-talk in calcium signalling: Evidence from nonstarved and long-term serum-deprived glioma C6 cells

    doi: 10.1007/s11302-007-9051-5

    Figure Lengend Snippet: The effect of AR-C69931MX, the P2Y 12 receptor antagonist, on the first and second phase of calcium response in cells grown in the presence of serum. a Cells in Ca 2+ -free buffer were stimulated by 10 μM ADP ( arrow ). Two cell groups were studied: the first experimental group was treated with 10 μM AR-C69931MX for 3 min before ADP addition and release of calcium from the ER ( light gray line , ARC - ADP), while the second experimental group was treated for 3 min with 10 μM AR-C69931MX just after the end of calcium transient caused by ADP-induced release from the ER ( dark gray line , ADP - ARC). The control cell group was induced with ADP, without use of AR-C69931MX ( black line , Ctrl). Then, in all cell groups, the medium was exchanged with fresh medium containing 2 mM CaCl 2 ( arrow ). b Strength of two phases of calcium response. Colour of bars as described above. Difference between bars marked with a minus sign is statistically insignificant

    Article Snippet: Antibodies recognizing P2Y 1 , P2Y 2 , and P2Y 12 were purchased from Alomone and Sigma Aldrich Chemical labs. Horseradish peroxidase-conjugated anti-rabbit IgG was from Cell Signalling.

    Techniques: