Journal: Physiological Reports
Article Title: Maxi‐K channel ( BK C a ) activity veils the myogenic tone of mesenteric artery in rats
Figure Lengend Snippet: Electrophysiological property of BK C a channel and qPCR analysis of Slo1 α , Slo β 1‐subunit from isolated CASMC s and MASMC s. (A) Upper: representative current traces in whole‐cell configuration with fixed intracellular Ca 2+ activity to 1 μ mol/L. Outward currents were mostly inhibited by 1 mmol/L TEA and 100 nmol/L iberiotoxin. Lower: summary of TEA ‐sensitive and iberiotoxin (Ibtx)‐sensitive BK C a current density in CASMC s and MASMC s ( ‡ P < 0.001). (B) Upper: representative unitary current recorded at +40 mV in inside‐out configuration with intracellular free Ca 2+ of 1 μ mol/L. Lower: unitary current–voltage (I–V) relationship of BK C a channels in CASMC s and MASMC s. (C) Normalized current–voltage relationship with maximum current at +80 mV in free Ca 2+ 30 μ mol/L concentration. (D) Summary of the normalized BK C a current at +20 mV in response to a three range of free Ca 2+ concentrations (3, 10 and 30 μ mol/L). (E) Bar graph showing the relative mRNA abundance of Slo1 α and Slo β 1 analyzed with 2 −∆∆Ct between CASMC s and MASMC s. (F) Ratio of Slo β 1: Slo1 α BK C a subunit mRNA expression between CASMCs and MASMCs.
Article Snippet: Then cells were blocked with 10% FBS for 1 h at room temperature, and incubated with primary antibodies of anti‐Slo1 α ‐subunit (1:50 diluted in 5% FBS, Alomone labs) or anti‐Slo β 1‐subunit (1:200 diluted in 5% FBS, Abcam) overnight at 4°C.
Techniques: Isolation, Activity Assay, Concentration Assay, Expressing