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Fig. 4. KCNE subunit protein expression in bovine RPE and neural retina. A. Western blot analysis of bovine retinal crude membrane proteins (ret; left panel) and RPE plasma membrane proteins (right panel) using antibodies against KCNE1 (Table 2). B. Western blot analysis of bovine retinal crude membrane proteins and RPE plasma membrane proteins (left panel) using antibodies against KCNE2 (Table 2). Bands were eliminated when the antibodies were preabsorbed with antigenic peptide (right panel). C. Western blot analysis of bovine skeletal muscle sarcolemma proteins (s.m.), retinal crude membrane proteins, and RPE plasma membrane proteins (left panel) using antibodies against <t>KCNE3</t> (Table 2). Bands were eliminated when the antibodies were preabsorbed with antigenic peptide (right panel). D. Western blot analysis of bovine skeletal muscle sarcolemma proteins, retinal crude membrane proteins, and RPE plasma membrane proteins using antibodies against <t>KCNE4</t> (Table 2).
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Fig. 4. KCNE subunit protein expression in bovine RPE and neural retina. A. Western blot analysis of bovine retinal crude membrane proteins (ret; left panel) and RPE plasma membrane proteins (right panel) using antibodies against KCNE1 (Table 2). B. Western blot analysis of bovine retinal crude membrane proteins and RPE plasma membrane proteins (left panel) using antibodies against KCNE2 (Table 2). Bands were eliminated when the antibodies were preabsorbed with antigenic peptide (right panel). C. Western blot analysis of bovine skeletal muscle sarcolemma proteins (s.m.), retinal crude membrane proteins, and RPE plasma membrane proteins (left panel) using antibodies against KCNE3 (Table 2). Bands were eliminated when the antibodies were preabsorbed with antigenic peptide (right panel). D. Western blot analysis of bovine skeletal muscle sarcolemma proteins, retinal crude membrane proteins, and RPE plasma membrane proteins using antibodies against KCNE4 (Table 2).

Journal: Experimental Eye Research

Article Title: KCNQ and KCNE potassium channel subunit expression in bovine retinal pigment epithelium

doi: 10.1016/j.exer.2013.10.013

Figure Lengend Snippet: Fig. 4. KCNE subunit protein expression in bovine RPE and neural retina. A. Western blot analysis of bovine retinal crude membrane proteins (ret; left panel) and RPE plasma membrane proteins (right panel) using antibodies against KCNE1 (Table 2). B. Western blot analysis of bovine retinal crude membrane proteins and RPE plasma membrane proteins (left panel) using antibodies against KCNE2 (Table 2). Bands were eliminated when the antibodies were preabsorbed with antigenic peptide (right panel). C. Western blot analysis of bovine skeletal muscle sarcolemma proteins (s.m.), retinal crude membrane proteins, and RPE plasma membrane proteins (left panel) using antibodies against KCNE3 (Table 2). Bands were eliminated when the antibodies were preabsorbed with antigenic peptide (right panel). D. Western blot analysis of bovine skeletal muscle sarcolemma proteins, retinal crude membrane proteins, and RPE plasma membrane proteins using antibodies against KCNE4 (Table 2).

Article Snippet: Antibody Source Catalog number Epitope Type KCNQ1 Santa Cruz Biotechnology sc-10646 C-terminal of human Rabbit polyclonal KCNQ4 Neuromab 75-082 N-terminal of human Mouse monoclonal KCNQ5 Chemicon AB5599 N-terminal of human KCNQ5 Rabbit polyclonal KCNE1 SigmaeAldrich WH0003753M1 aa 67e130 of human KCNE1 Mouse monoclonal KCNE1 Alomone Labs APC-008 aa 67e129 of human KCNE1 Rabbit polyclonal KCNE1 Santa Cruz Biotechnology SC-16796 N-16 Internal region of human KCNE1 Goat polyclonal KCNE2 Alomone Labs APC-054 aa 88e107 of rat KCNE2 Rabbit polyclonal KCNE3 Alomone Labs APC-118 aa 81e93 of human KCNE3 Rabbit polyclonal KCNE4 Gift from Dr. A.L.

Techniques: Expressing, Western Blot, Membrane, Clinical Proteomics