Journal: Frontiers in Physiology
Article Title: Activation of Orexin 1 Receptors in the Paraventricular Nucleus Contributes to the Development of Deoxycorticosterone Acetate-Salt Hypertension Through Regulation of Vasopressin
Figure Lengend Snippet: The hypothesized relationship mediating the impact of central orexin system functioning on hypertension development in the DOCA-salt rat model. DOCA-salt treatment results in activation of circumventricular organs (CVOs), which send projections to the lateral hypothalamus (LH), stimulating Orexin A (OXA) release. OXA then interacts with OX1R at the PVN. This then facilitates an increased production and secretion of AVP to the periphery, where it causes increased blood pressure through various means including vasoconstriction, fluid reabsorption, and increased blood volume. CVO, circumventricular organs; LH, lateral hypothalamus; OXA, orexin-A; OX1R, orexin 1-receptor; PVN, paraventricular nucleus; and AVP, arginine vasopressin.
Article Snippet: Following wash in 1xPBS 3 times for 10 min each, brain sections were incubated with either rabbit anti-OX1R antibody (Alomon Laboratories, Jerusalem, Israel, 1:300 dilution), rabbit anti-AVP antibody (OriGene Technologies, United States, 1:400 dilution), or mouse anti-OXA antibody (Abcam, United States, 1:300 dilution) in PBS containing 0.5% Triton X-100 and 5% horse serum for 72 h at 4°C.
Techniques: Activation Assay