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    Alomone Labs anti orexin receptor 1 antibody
    Paraventricular nucleus bilateral microinjection of <t>AAV2-OX1R-shRNA</t> significantly reduces OX1R expression within the brain. Following 3 weeks of DOCA-salt treatment, rat PVN areas were collected and subjected to either PCR analysis or immunostaining. mRNA levels of OX1R (A) were significantly reduced following OX1R knockdown ( n = 5; * p
    Anti Orexin Receptor 1 Antibody, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Paraventricular nucleus bilateral microinjection of AAV2-OX1R-shRNA significantly reduces OX1R expression within the brain. Following 3 weeks of DOCA-salt treatment, rat PVN areas were collected and subjected to either PCR analysis or immunostaining. mRNA levels of OX1R (A) were significantly reduced following OX1R knockdown ( n = 5; * p

    Journal: Frontiers in Physiology

    Article Title: Activation of Orexin 1 Receptors in the Paraventricular Nucleus Contributes to the Development of Deoxycorticosterone Acetate-Salt Hypertension Through Regulation of Vasopressin

    doi: 10.3389/fphys.2021.641331

    Figure Lengend Snippet: Paraventricular nucleus bilateral microinjection of AAV2-OX1R-shRNA significantly reduces OX1R expression within the brain. Following 3 weeks of DOCA-salt treatment, rat PVN areas were collected and subjected to either PCR analysis or immunostaining. mRNA levels of OX1R (A) were significantly reduced following OX1R knockdown ( n = 5; * p

    Article Snippet: Following wash in 1xPBS 3 times for 10 min each, brain sections were incubated with either rabbit anti-OX1R antibody (Alomon Laboratories, Jerusalem, Israel, 1:300 dilution), rabbit anti-AVP antibody (OriGene Technologies, United States, 1:400 dilution), or mouse anti-OXA antibody (Abcam, United States, 1:300 dilution) in PBS containing 0.5% Triton X-100 and 5% horse serum for 72 h at 4°C.

    Techniques: shRNA, Expressing, Polymerase Chain Reaction, Immunostaining

    Paraventricular nucleus bilateral microinjection of AAV2-OX1R-shRNA significantly reduces central production and subsequent peripheral release of AVP. Following 3 weeks of DOCA-salt treatment, brain PVN areas as well as plasma were collected to test AVP central expression and peripheral secretion. PVN OX1R knockdown ( n = 5) resulted in a significantly reduced PVN AVP mRNA expression (A) compared to DOCA-salt treated rats ( n = 9; * p

    Journal: Frontiers in Physiology

    Article Title: Activation of Orexin 1 Receptors in the Paraventricular Nucleus Contributes to the Development of Deoxycorticosterone Acetate-Salt Hypertension Through Regulation of Vasopressin

    doi: 10.3389/fphys.2021.641331

    Figure Lengend Snippet: Paraventricular nucleus bilateral microinjection of AAV2-OX1R-shRNA significantly reduces central production and subsequent peripheral release of AVP. Following 3 weeks of DOCA-salt treatment, brain PVN areas as well as plasma were collected to test AVP central expression and peripheral secretion. PVN OX1R knockdown ( n = 5) resulted in a significantly reduced PVN AVP mRNA expression (A) compared to DOCA-salt treated rats ( n = 9; * p

    Article Snippet: Following wash in 1xPBS 3 times for 10 min each, brain sections were incubated with either rabbit anti-OX1R antibody (Alomon Laboratories, Jerusalem, Israel, 1:300 dilution), rabbit anti-AVP antibody (OriGene Technologies, United States, 1:400 dilution), or mouse anti-OXA antibody (Abcam, United States, 1:300 dilution) in PBS containing 0.5% Triton X-100 and 5% horse serum for 72 h at 4°C.

    Techniques: shRNA, Expressing

    Chronic knockdown of OX1R in the PVN attenuates increased mean arterial pressure (MAP) induced by DOCA-salt treatment in SD rats. Rats were bilaterally injected with AAV2-OX1R-shRNA into the PVN 2 weeks prior to DOCA-salt treatment. Following this, blood pressure was monitored for 3 weeks during DOCA-salt treatment. Central knockdown of OX1R ( n = 4) within the PVN results in attenuation of mean arterial pressure compared to DOCA-salt treated rats ( n = 4; A ; * p

    Journal: Frontiers in Physiology

    Article Title: Activation of Orexin 1 Receptors in the Paraventricular Nucleus Contributes to the Development of Deoxycorticosterone Acetate-Salt Hypertension Through Regulation of Vasopressin

    doi: 10.3389/fphys.2021.641331

    Figure Lengend Snippet: Chronic knockdown of OX1R in the PVN attenuates increased mean arterial pressure (MAP) induced by DOCA-salt treatment in SD rats. Rats were bilaterally injected with AAV2-OX1R-shRNA into the PVN 2 weeks prior to DOCA-salt treatment. Following this, blood pressure was monitored for 3 weeks during DOCA-salt treatment. Central knockdown of OX1R ( n = 4) within the PVN results in attenuation of mean arterial pressure compared to DOCA-salt treated rats ( n = 4; A ; * p

    Article Snippet: Following wash in 1xPBS 3 times for 10 min each, brain sections were incubated with either rabbit anti-OX1R antibody (Alomon Laboratories, Jerusalem, Israel, 1:300 dilution), rabbit anti-AVP antibody (OriGene Technologies, United States, 1:400 dilution), or mouse anti-OXA antibody (Abcam, United States, 1:300 dilution) in PBS containing 0.5% Triton X-100 and 5% horse serum for 72 h at 4°C.

    Techniques: Injection, shRNA

    Deoxycorticosterone acetate (DOCA)-salt treatment increases orexin 1-receptor (OX1R) expression in the paraventricular nucleus (PVN) of Sprague Dawley (SD) rats. OX1R mRNA (A) and immunofluorescence (B) was compared in the PVN area between DOCA-salt treated ( n = 4) and vehicle control ( n = 3) rats. DOCA-salt treated rats showed a significant increase in OX1R expression within the PVN area ( * p

    Journal: Frontiers in Physiology

    Article Title: Activation of Orexin 1 Receptors in the Paraventricular Nucleus Contributes to the Development of Deoxycorticosterone Acetate-Salt Hypertension Through Regulation of Vasopressin

    doi: 10.3389/fphys.2021.641331

    Figure Lengend Snippet: Deoxycorticosterone acetate (DOCA)-salt treatment increases orexin 1-receptor (OX1R) expression in the paraventricular nucleus (PVN) of Sprague Dawley (SD) rats. OX1R mRNA (A) and immunofluorescence (B) was compared in the PVN area between DOCA-salt treated ( n = 4) and vehicle control ( n = 3) rats. DOCA-salt treated rats showed a significant increase in OX1R expression within the PVN area ( * p

    Article Snippet: Following wash in 1xPBS 3 times for 10 min each, brain sections were incubated with either rabbit anti-OX1R antibody (Alomon Laboratories, Jerusalem, Israel, 1:300 dilution), rabbit anti-AVP antibody (OriGene Technologies, United States, 1:400 dilution), or mouse anti-OXA antibody (Abcam, United States, 1:300 dilution) in PBS containing 0.5% Triton X-100 and 5% horse serum for 72 h at 4°C.

    Techniques: Expressing, Immunofluorescence

    The hypothesized relationship mediating the impact of central orexin system functioning on hypertension development in the DOCA-salt rat model. DOCA-salt treatment results in activation of circumventricular organs (CVOs), which send projections to the lateral hypothalamus (LH), stimulating Orexin A (OXA) release. OXA then interacts with OX1R at the PVN. This then facilitates an increased production and secretion of AVP to the periphery, where it causes increased blood pressure through various means including vasoconstriction, fluid reabsorption, and increased blood volume. CVO, circumventricular organs; LH, lateral hypothalamus; OXA, orexin-A; OX1R, orexin 1-receptor; PVN, paraventricular nucleus; and AVP, arginine vasopressin.

    Journal: Frontiers in Physiology

    Article Title: Activation of Orexin 1 Receptors in the Paraventricular Nucleus Contributes to the Development of Deoxycorticosterone Acetate-Salt Hypertension Through Regulation of Vasopressin

    doi: 10.3389/fphys.2021.641331

    Figure Lengend Snippet: The hypothesized relationship mediating the impact of central orexin system functioning on hypertension development in the DOCA-salt rat model. DOCA-salt treatment results in activation of circumventricular organs (CVOs), which send projections to the lateral hypothalamus (LH), stimulating Orexin A (OXA) release. OXA then interacts with OX1R at the PVN. This then facilitates an increased production and secretion of AVP to the periphery, where it causes increased blood pressure through various means including vasoconstriction, fluid reabsorption, and increased blood volume. CVO, circumventricular organs; LH, lateral hypothalamus; OXA, orexin-A; OX1R, orexin 1-receptor; PVN, paraventricular nucleus; and AVP, arginine vasopressin.

    Article Snippet: Following wash in 1xPBS 3 times for 10 min each, brain sections were incubated with either rabbit anti-OX1R antibody (Alomon Laboratories, Jerusalem, Israel, 1:300 dilution), rabbit anti-AVP antibody (OriGene Technologies, United States, 1:400 dilution), or mouse anti-OXA antibody (Abcam, United States, 1:300 dilution) in PBS containing 0.5% Triton X-100 and 5% horse serum for 72 h at 4°C.

    Techniques: Activation Assay