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    Alomone Labs trpc6 specific antiserum
    Vascular smooth muscle properties in wild-type and <t>TRPC6</t> − / − mice. Agonist-induced vasoconstriction of aortic rings (A and B) and mesenteric arteries (C), telemetric measurement of mean blood pressure (D), and vasoconstriction in response
    Trpc6 Specific Antiserum, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Vascular smooth muscle properties in wild-type and TRPC6 − / − mice. Agonist-induced vasoconstriction of aortic rings (A and B) and mesenteric arteries (C), telemetric measurement of mean blood pressure (D), and vasoconstriction in response

    Journal:

    Article Title: Increased Vascular Smooth Muscle Contractility in TRPC6−/− Mice

    doi: 10.1128/MCB.25.16.6980-6989.2005

    Figure Lengend Snippet: Vascular smooth muscle properties in wild-type and TRPC6 − / − mice. Agonist-induced vasoconstriction of aortic rings (A and B) and mesenteric arteries (C), telemetric measurement of mean blood pressure (D), and vasoconstriction in response

    Article Snippet: PCRs were carried out using the following conditions: initial denaturation for 3 min at 94°C and 45 cycles of 94°C for 30 s, 55°C for 30 s, and 72°C for 1 min, followed by a final extension at 72°C for 7 min. Western blot analysis of proteins from embryonic brain was carried out as described previously ( ) using a TRPC6-specific antiserum (Alomone Labs, Jerusalem, Israel).

    Techniques: Mouse Assay

    Targeted disruption of the murine TRPC6 gene. (A) cDNA with delineation of intron-exon boundaries and location of hydrophobic segments presumed to code for transmembrane domains, genomic organization of the TRPC6 gene, and restriction maps of the mTRPC6

    Journal:

    Article Title: Increased Vascular Smooth Muscle Contractility in TRPC6−/− Mice

    doi: 10.1128/MCB.25.16.6980-6989.2005

    Figure Lengend Snippet: Targeted disruption of the murine TRPC6 gene. (A) cDNA with delineation of intron-exon boundaries and location of hydrophobic segments presumed to code for transmembrane domains, genomic organization of the TRPC6 gene, and restriction maps of the mTRPC6

    Article Snippet: PCRs were carried out using the following conditions: initial denaturation for 3 min at 94°C and 45 cycles of 94°C for 30 s, 55°C for 30 s, and 72°C for 1 min, followed by a final extension at 72°C for 7 min. Western blot analysis of proteins from embryonic brain was carried out as described previously ( ) using a TRPC6-specific antiserum (Alomone Labs, Jerusalem, Israel).

    Techniques:

    Ba 2+ influx into isolated smooth muscle cells from control and TRPC6 − / − mice. (A) Ba 2+ influx into isolated smooth muscle cells from thoracic aortas of control and TRPC6 − / − mice. Isolated cells were loaded

    Journal:

    Article Title: Increased Vascular Smooth Muscle Contractility in TRPC6−/− Mice

    doi: 10.1128/MCB.25.16.6980-6989.2005

    Figure Lengend Snippet: Ba 2+ influx into isolated smooth muscle cells from control and TRPC6 − / − mice. (A) Ba 2+ influx into isolated smooth muscle cells from thoracic aortas of control and TRPC6 − / − mice. Isolated cells were loaded

    Article Snippet: PCRs were carried out using the following conditions: initial denaturation for 3 min at 94°C and 45 cycles of 94°C for 30 s, 55°C for 30 s, and 72°C for 1 min, followed by a final extension at 72°C for 7 min. Western blot analysis of proteins from embryonic brain was carried out as described previously ( ) using a TRPC6-specific antiserum (Alomone Labs, Jerusalem, Israel).

    Techniques: Isolation, Mouse Assay

    Quantitative analysis of mRNA levels by real-time PCR of total RNA from thoracic aortas (A) and cerebral arteries (B). Total RNA was prepared from WT (thoracic aortas, n = 4; cerebral arteries, n = 4, black bars) and TRPC6 − / −

    Journal:

    Article Title: Increased Vascular Smooth Muscle Contractility in TRPC6−/− Mice

    doi: 10.1128/MCB.25.16.6980-6989.2005

    Figure Lengend Snippet: Quantitative analysis of mRNA levels by real-time PCR of total RNA from thoracic aortas (A) and cerebral arteries (B). Total RNA was prepared from WT (thoracic aortas, n = 4; cerebral arteries, n = 4, black bars) and TRPC6 − / −

    Article Snippet: PCRs were carried out using the following conditions: initial denaturation for 3 min at 94°C and 45 cycles of 94°C for 30 s, 55°C for 30 s, and 72°C for 1 min, followed by a final extension at 72°C for 7 min. Western blot analysis of proteins from embryonic brain was carried out as described previously ( ) using a TRPC6-specific antiserum (Alomone Labs, Jerusalem, Israel).

    Techniques: Real-time Polymerase Chain Reaction

    (A and B) Analysis of current densities by electrophysiological analysis of single smooth muscle cells isolated from cerebral arteries of control (black traces) or TRPC6 − / − (gray traces) mice in the absence (A, left panel) or presence

    Journal:

    Article Title: Increased Vascular Smooth Muscle Contractility in TRPC6−/− Mice

    doi: 10.1128/MCB.25.16.6980-6989.2005

    Figure Lengend Snippet: (A and B) Analysis of current densities by electrophysiological analysis of single smooth muscle cells isolated from cerebral arteries of control (black traces) or TRPC6 − / − (gray traces) mice in the absence (A, left panel) or presence

    Article Snippet: PCRs were carried out using the following conditions: initial denaturation for 3 min at 94°C and 45 cycles of 94°C for 30 s, 55°C for 30 s, and 72°C for 1 min, followed by a final extension at 72°C for 7 min. Western blot analysis of proteins from embryonic brain was carried out as described previously ( ) using a TRPC6-specific antiserum (Alomone Labs, Jerusalem, Israel).

    Techniques: Isolation, Mouse Assay