Journal: Journal of Neurology

Article Title: Different pain phenotypes are associated with anti-Caspr2 autoantibodies

doi: 10.1007/s00415-024-12224-4

Figure Lengend Snippet: Overview on demographic data, IgG subclasses, CSF findings and concomitant diseases in anti-Caspr2-positive patients with pain and without pain

Article Snippet: After two days, the transfected cells were incubated with patient serum (1:250, one serum that was negative at 1:250 was repeated with a dilution of 1:100) and a commercial anti-Caspr2 antibody from sheep (1:250, R&D Systems by Bio-Techne, AF5145) for one hour.

Techniques:

Journal: Journal of Neurology

Article Title: Different pain phenotypes are associated with anti-Caspr2 autoantibodies

doi: 10.1007/s00415-024-12224-4

Figure Lengend Snippet: Patient anti-Caspr2 autoantibodies show distinct IgG subclass pattern independent of the pain phenotype. HEK293 cells were transfected with human Caspr2. A Caspr2 was stained with a commercial antibody (1:250; cyan; upper left image). Secondary FITC-coupled IgG subclass antibodies (IgG1-4) were tested with healthy control (HC, 1:250) serum instead of patient (pat) serum as negative controls (upper lane, right images). Representative images following incubation with human patient serum 13 (1:250) detected by secondary antibodies against total human IgG (red; lower left image). Serum of patient 13 (1:50 for subclass determination) was positive for all four IgG subclasses but with different intensities (IgG3 < IgG1 < IgG2 = IgG4; cyan, lower right images). DAPI marks the nuclei. Scale bar refers to 10 µm. B Intensity plot of IgG subclass determination from all patients (40 total) investigated. Intensities were classified from 0–1-2–3 (white—light blue—blue—dark blue; no staining—very weak but visible staining—intense staining—very intense staining). Note, almost all sera contained Caspr2 autoantibodies of subclass IgG4. In ( C ), the probability of different variables of cluster analysis in cluster 1 and 2 is depicted. Patients of cluster 1 suffer from mostly distal burning and tingling pain that is increased by exercise and cold whereas cluster 2 is characterized by widespread pain, myalgia and cramps. pat patient, HC healthy control

Article Snippet: After two days, the transfected cells were incubated with patient serum (1:250, one serum that was negative at 1:250 was repeated with a dilution of 1:100) and a commercial anti-Caspr2 antibody from sheep (1:250, R&D Systems by Bio-Techne, AF5145) for one hour.

Techniques: Transfection, Staining, Control, Incubation

Journal: Journal of Neurology

Article Title: Different pain phenotypes are associated with anti-Caspr2 autoantibodies

doi: 10.1007/s00415-024-12224-4

Figure Lengend Snippet: Pain characteristics of anti-Caspr2-positive patients

Article Snippet: After two days, the transfected cells were incubated with patient serum (1:250, one serum that was negative at 1:250 was repeated with a dilution of 1:100) and a commercial anti-Caspr2 antibody from sheep (1:250, R&D Systems by Bio-Techne, AF5145) for one hour.

Techniques: