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    Alomone Labs anti cav3 3 cacna1i antibody
    In vivo and in vitro expression of <t>Cav3.3:</t> Immunostaining of Cav3.3 in dissociated PCs at 2div ( A – D ), and in cryosections of rat cerebella at p0 ( E – H ) and p9 ( I – L ). Cav3.3 was stained green, calbindin red, and nuclear staining was performed with Hoechst (blue). In the PC culture, Cav3.3 was localized at the soma and along the dendrites or axons, whereas in cryosections of p0, cerebella staining of Cav3.3 was visible mainly near to the soma. In 9-day-old PCs, the Cav3.3-antibody strongly marked the ML, where the PC dendrites are localized. eGCL = external granular cell layer, ML = molecular layer, PCL = PC layer, iGCL = internal granular cell layer. Scale bar: 20 µm ( A , B , E , F , I , J ) and 2 µm ( C , D , G , H , K , L ).
    Anti Cav3 3 Cacna1i Antibody, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti cav3 3 cacna1i antibody/product/Alomone Labs
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti cav3 3 cacna1i antibody - by Bioz Stars, 2022-08
    94/100 stars
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    In vivo and in vitro expression of Cav3.3: Immunostaining of Cav3.3 in dissociated PCs at 2div ( A – D ), and in cryosections of rat cerebella at p0 ( E – H ) and p9 ( I – L ). Cav3.3 was stained green, calbindin red, and nuclear staining was performed with Hoechst (blue). In the PC culture, Cav3.3 was localized at the soma and along the dendrites or axons, whereas in cryosections of p0, cerebella staining of Cav3.3 was visible mainly near to the soma. In 9-day-old PCs, the Cav3.3-antibody strongly marked the ML, where the PC dendrites are localized. eGCL = external granular cell layer, ML = molecular layer, PCL = PC layer, iGCL = internal granular cell layer. Scale bar: 20 µm ( A , B , E , F , I , J ) and 2 µm ( C , D , G , H , K , L ).

    Journal: Cells

    Article Title: Expression Pattern of T-Type Ca2+ Channels in Cerebellar Purkinje Cells after VEGF Treatment

    doi: 10.3390/cells10092277

    Figure Lengend Snippet: In vivo and in vitro expression of Cav3.3: Immunostaining of Cav3.3 in dissociated PCs at 2div ( A – D ), and in cryosections of rat cerebella at p0 ( E – H ) and p9 ( I – L ). Cav3.3 was stained green, calbindin red, and nuclear staining was performed with Hoechst (blue). In the PC culture, Cav3.3 was localized at the soma and along the dendrites or axons, whereas in cryosections of p0, cerebella staining of Cav3.3 was visible mainly near to the soma. In 9-day-old PCs, the Cav3.3-antibody strongly marked the ML, where the PC dendrites are localized. eGCL = external granular cell layer, ML = molecular layer, PCL = PC layer, iGCL = internal granular cell layer. Scale bar: 20 µm ( A , B , E , F , I , J ) and 2 µm ( C , D , G , H , K , L ).

    Article Snippet: On the next day, the samples were washed with PBS three times for 10 min and incubated with secondary anti-mouse TRITC antibody (goat, 1:1000 T5393, Sigma-Aldrich) at room temperature for 2 h, respectively, 2.5 h. After additional washing, the second primary antibody anti-Cav3.1 (rabbit, 1:1500 ACC21, Alomone Labs), anti-Cav3.2 (Rabbit, 1:1500 ACC25, Alomone Labs) or anti-Cav3.3 (rabbit, 1:1000 ACC-009, Alomone Labs) was applied at 4 °C overnight.

    Techniques: In Vivo, In Vitro, Expressing, Immunostaining, Staining

    In vivo expression of Cacna1g , Cacna1h , and Cacna1i : Examination of the relative mRNA expression pattern of Cacna1g , Cacna1h , and Cacna1i in PCs was performed at p9 and p30 via qPCR of LMD samples. The values were normalized against the values of the PCs of p9, and Gapdh was the housekeeping gene. Note that 1,000,000 μm 2 of enriched PCs from 20 different rat cerebella were collected. The analysis was performed with the help of the 2 −ΔΔct method. Significant differences were indicated by *** p

    Journal: Cells

    Article Title: Expression Pattern of T-Type Ca2+ Channels in Cerebellar Purkinje Cells after VEGF Treatment

    doi: 10.3390/cells10092277

    Figure Lengend Snippet: In vivo expression of Cacna1g , Cacna1h , and Cacna1i : Examination of the relative mRNA expression pattern of Cacna1g , Cacna1h , and Cacna1i in PCs was performed at p9 and p30 via qPCR of LMD samples. The values were normalized against the values of the PCs of p9, and Gapdh was the housekeeping gene. Note that 1,000,000 μm 2 of enriched PCs from 20 different rat cerebella were collected. The analysis was performed with the help of the 2 −ΔΔct method. Significant differences were indicated by *** p

    Article Snippet: On the next day, the samples were washed with PBS three times for 10 min and incubated with secondary anti-mouse TRITC antibody (goat, 1:1000 T5393, Sigma-Aldrich) at room temperature for 2 h, respectively, 2.5 h. After additional washing, the second primary antibody anti-Cav3.1 (rabbit, 1:1500 ACC21, Alomone Labs), anti-Cav3.2 (Rabbit, 1:1500 ACC25, Alomone Labs) or anti-Cav3.3 (rabbit, 1:1000 ACC-009, Alomone Labs) was applied at 4 °C overnight.

    Techniques: In Vivo, Expressing, Real-time Polymerase Chain Reaction, Laser Capture Microdissection

    Effect of VEGF on Cacna1g , Cacna1h , and Cacna1i expression: The graphs show the relative mRNA expression of Cacna1g , Cacna1h , and Cacna1i with or without VEGF treatment for 24 h and 48 h. The values were normalised against the control group and the housekeeping gene was Gapdh . n = 8, significant differences are indicated by * p

    Journal: Cells

    Article Title: Expression Pattern of T-Type Ca2+ Channels in Cerebellar Purkinje Cells after VEGF Treatment

    doi: 10.3390/cells10092277

    Figure Lengend Snippet: Effect of VEGF on Cacna1g , Cacna1h , and Cacna1i expression: The graphs show the relative mRNA expression of Cacna1g , Cacna1h , and Cacna1i with or without VEGF treatment for 24 h and 48 h. The values were normalised against the control group and the housekeeping gene was Gapdh . n = 8, significant differences are indicated by * p

    Article Snippet: On the next day, the samples were washed with PBS three times for 10 min and incubated with secondary anti-mouse TRITC antibody (goat, 1:1000 T5393, Sigma-Aldrich) at room temperature for 2 h, respectively, 2.5 h. After additional washing, the second primary antibody anti-Cav3.1 (rabbit, 1:1500 ACC21, Alomone Labs), anti-Cav3.2 (Rabbit, 1:1500 ACC25, Alomone Labs) or anti-Cav3.3 (rabbit, 1:1000 ACC-009, Alomone Labs) was applied at 4 °C overnight.

    Techniques: Expressing