Article Title: Expression Pattern of T-Type Ca2+ Channels in Cerebellar Purkinje Cells after VEGF Treatment
Figure Lengend Snippet: In vivo and in vitro expression of Cav3.3: Immunostaining of Cav3.3 in dissociated PCs at 2div ( A – D ), and in cryosections of rat cerebella at p0 ( E – H ) and p9 ( I – L ). Cav3.3 was stained green, calbindin red, and nuclear staining was performed with Hoechst (blue). In the PC culture, Cav3.3 was localized at the soma and along the dendrites or axons, whereas in cryosections of p0, cerebella staining of Cav3.3 was visible mainly near to the soma. In 9-day-old PCs, the Cav3.3-antibody strongly marked the ML, where the PC dendrites are localized. eGCL = external granular cell layer, ML = molecular layer, PCL = PC layer, iGCL = internal granular cell layer. Scale bar: 20 µm ( A , B , E , F , I , J ) and 2 µm ( C , D , G , H , K , L ).
Article Snippet: On the next day, the samples were washed with PBS three times for 10 min and incubated with secondary anti-mouse TRITC antibody (goat, 1:1000 T5393, Sigma-Aldrich) at room temperature for 2 h, respectively, 2.5 h. After additional washing, the second primary antibody anti-Cav3.1 (rabbit, 1:1500 ACC21, Alomone Labs), anti-Cav3.2 (Rabbit, 1:1500 ACC25, Alomone Labs) or anti-Cav3.3 (rabbit, 1:1000 ACC-009, Alomone Labs) was applied at 4 °C overnight.
Techniques: In Vivo, In Vitro, Expressing, Immunostaining, Staining