AB-220-NA Search Results


polyclonal anti pdgf bb antibodies  (R&D Systems)
94
R&D Systems polyclonal anti pdgf bb antibodies
FIGURE 1. A, representative gelatin-based zymograms of the effect of hypoxia (H) on MMP-2 and MMP-9 expression by fibroblasts and VSMCs compared with normoxia (N). Treatment of cell culture medium with 20 mMEDTA(24h)abolishedtheexpressionofpro-MMP-2andpro-MMP-9.Arrowsindicatethemigrationposition of purified pro-MMP-2 and pro-MMP-9. B, densitometric analysis of pro-MMP-2 expression by hypoxia in human primary lung fibroblast cell lines (n 8) and the effect of <t>PDGF-BB</t> and the underlying intracellular signaling inhibition. PD98059 inhibits Erk1/2 MAP kinase, SB203580 inhibits p38 MAP kinase, and SB202474 is a negative control for SB203580. C, densitometric analysis of pro-MMP-2 expression by hypoxia in human primary VSMC lines (n 8) and the effect of PDGF-BB and the underlying intracellular signaling pathways. Values are presented as the percentage of control (normoxia 24 h), and each bar represents the mean S.D. of six independent experiments.
Polyclonal Anti Pdgf Bb Antibodies, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/polyclonal anti pdgf bb antibodies/product/R&D Systems
Average 94 stars, based on 1 article reviews
polyclonal anti pdgf bb antibodies - by Bioz Stars, 2026-02
94/100 stars
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goat anti human pdgf bb ab neutralizing igg  (R&D Systems)
93
R&D Systems goat anti human pdgf bb ab neutralizing igg
FIGURE 1. A, representative gelatin-based zymograms of the effect of hypoxia (H) on MMP-2 and MMP-9 expression by fibroblasts and VSMCs compared with normoxia (N). Treatment of cell culture medium with 20 mMEDTA(24h)abolishedtheexpressionofpro-MMP-2andpro-MMP-9.Arrowsindicatethemigrationposition of purified pro-MMP-2 and pro-MMP-9. B, densitometric analysis of pro-MMP-2 expression by hypoxia in human primary lung fibroblast cell lines (n 8) and the effect of <t>PDGF-BB</t> and the underlying intracellular signaling inhibition. PD98059 inhibits Erk1/2 MAP kinase, SB203580 inhibits p38 MAP kinase, and SB202474 is a negative control for SB203580. C, densitometric analysis of pro-MMP-2 expression by hypoxia in human primary VSMC lines (n 8) and the effect of PDGF-BB and the underlying intracellular signaling pathways. Values are presented as the percentage of control (normoxia 24 h), and each bar represents the mean S.D. of six independent experiments.
Goat Anti Human Pdgf Bb Ab Neutralizing Igg, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/goat anti human pdgf bb ab neutralizing igg/product/R&D Systems
Average 93 stars, based on 1 article reviews
goat anti human pdgf bb ab neutralizing igg - by Bioz Stars, 2026-02
93/100 stars
  Buy from Supplier

Image Search Results


FIGURE 1. A, representative gelatin-based zymograms of the effect of hypoxia (H) on MMP-2 and MMP-9 expression by fibroblasts and VSMCs compared with normoxia (N). Treatment of cell culture medium with 20 mMEDTA(24h)abolishedtheexpressionofpro-MMP-2andpro-MMP-9.Arrowsindicatethemigrationposition of purified pro-MMP-2 and pro-MMP-9. B, densitometric analysis of pro-MMP-2 expression by hypoxia in human primary lung fibroblast cell lines (n 8) and the effect of PDGF-BB and the underlying intracellular signaling inhibition. PD98059 inhibits Erk1/2 MAP kinase, SB203580 inhibits p38 MAP kinase, and SB202474 is a negative control for SB203580. C, densitometric analysis of pro-MMP-2 expression by hypoxia in human primary VSMC lines (n 8) and the effect of PDGF-BB and the underlying intracellular signaling pathways. Values are presented as the percentage of control (normoxia 24 h), and each bar represents the mean S.D. of six independent experiments.

Journal: Journal of Biological Chemistry

Article Title: Cell Type-specific Effect of Hypoxia and Platelet-derived Growth Factor-BB on Extracellular Matrix Turnover and Its Consequences for Lung Remodeling

doi: 10.1074/jbc.m602178200

Figure Lengend Snippet: FIGURE 1. A, representative gelatin-based zymograms of the effect of hypoxia (H) on MMP-2 and MMP-9 expression by fibroblasts and VSMCs compared with normoxia (N). Treatment of cell culture medium with 20 mMEDTA(24h)abolishedtheexpressionofpro-MMP-2andpro-MMP-9.Arrowsindicatethemigrationposition of purified pro-MMP-2 and pro-MMP-9. B, densitometric analysis of pro-MMP-2 expression by hypoxia in human primary lung fibroblast cell lines (n 8) and the effect of PDGF-BB and the underlying intracellular signaling inhibition. PD98059 inhibits Erk1/2 MAP kinase, SB203580 inhibits p38 MAP kinase, and SB202474 is a negative control for SB203580. C, densitometric analysis of pro-MMP-2 expression by hypoxia in human primary VSMC lines (n 8) and the effect of PDGF-BB and the underlying intracellular signaling pathways. Values are presented as the percentage of control (normoxia 24 h), and each bar represents the mean S.D. of six independent experiments.

Article Snippet: The effect of the MMP inhibitor OO-Hy (catalog number 44244; Calbiochem, Lucern, Switzerland) and of neutralizing polyclonal anti-PDGF-BB antibodies (AB-220-NA; R & D Systems) on proliferation (48 h) was also investigated.

Techniques: Expressing, Cell Culture, Purification, Inhibition, Negative Control, Protein-Protein interactions, Control

FIGURE 2. The effect of hypoxia and/or PDGF-BB on TIMP-1 (A) and TIMP-2 (B) by human lung fibroblasts and VSMCs within 48 h was assessed by ELISA. The role of Erk1/2 and p38 MAP kinase on the stimulatory effect of both TIMPs was also determined using PD98059-inhibiting Erk1/2 MAP kinase, SB203580-inhibiting p38 MAP kinase,andSB202474asanegativecontrolforSB203580.Eachbarrepresentsthemean S.D.ofsixindepend- ent experiments, each in triplicate. The control was defined as normoxia (24 h). C, aliquots of cell culture medium from human lung fibroblasts and VSMCs containing the same amount of protein were incubated in the absence () or in the presence () of 4-aminophenylmercuric acetate (APMA; 1 mM, 37 °C, 24 h) and subjected to gelatin zymography. Arrows indicate the migration position of purified pro-MMP-2 and active MMP-2. N, normoxia; H, hypoxia).

Journal: Journal of Biological Chemistry

Article Title: Cell Type-specific Effect of Hypoxia and Platelet-derived Growth Factor-BB on Extracellular Matrix Turnover and Its Consequences for Lung Remodeling

doi: 10.1074/jbc.m602178200

Figure Lengend Snippet: FIGURE 2. The effect of hypoxia and/or PDGF-BB on TIMP-1 (A) and TIMP-2 (B) by human lung fibroblasts and VSMCs within 48 h was assessed by ELISA. The role of Erk1/2 and p38 MAP kinase on the stimulatory effect of both TIMPs was also determined using PD98059-inhibiting Erk1/2 MAP kinase, SB203580-inhibiting p38 MAP kinase,andSB202474asanegativecontrolforSB203580.Eachbarrepresentsthemean S.D.ofsixindepend- ent experiments, each in triplicate. The control was defined as normoxia (24 h). C, aliquots of cell culture medium from human lung fibroblasts and VSMCs containing the same amount of protein were incubated in the absence () or in the presence () of 4-aminophenylmercuric acetate (APMA; 1 mM, 37 °C, 24 h) and subjected to gelatin zymography. Arrows indicate the migration position of purified pro-MMP-2 and active MMP-2. N, normoxia; H, hypoxia).

Article Snippet: The effect of the MMP inhibitor OO-Hy (catalog number 44244; Calbiochem, Lucern, Switzerland) and of neutralizing polyclonal anti-PDGF-BB antibodies (AB-220-NA; R & D Systems) on proliferation (48 h) was also investigated.

Techniques: Enzyme-linked Immunosorbent Assay, Control, Cell Culture, Incubation, Zymography, Migration, Purification

FIGURE 3. A, the effect of hypoxia (black bars) and PDGF-BB (striped bars) on MMP-1 expression by fibroblasts and VSMC within 48 h was assessed by ELISA. Each bar represents the mean S.D. of triplicates in six inde- pendent experiments. B, a representative immunoblot demonstrates the role of Erk1/2 and p38 MAP kinase in thehypoxia-inducedexpressionofMMP-1,MMP-13,andhypoxia-induciblefactor-1(HIF-1)aswellasonthe antagonizing effect of PDGF-BB in human lung fibroblasts. PD98059 inhibits Erk1/2 MAP kinase, SB203580 inhibits p38 MAP kinase and SB202474 is a negative control for SB203580. Ab, neutralizing antibody; control t0, protein extract from untreated cells before stimulation. C, hypoxia- and PDGF-BB-induced MMP-13 expression by fibroblasts but not by VSMC within 48 h. Each bar represents the mean S.D. of triplicate determinations from six independent experiments. Similar results were obtained with two additional cell lines.

Journal: Journal of Biological Chemistry

Article Title: Cell Type-specific Effect of Hypoxia and Platelet-derived Growth Factor-BB on Extracellular Matrix Turnover and Its Consequences for Lung Remodeling

doi: 10.1074/jbc.m602178200

Figure Lengend Snippet: FIGURE 3. A, the effect of hypoxia (black bars) and PDGF-BB (striped bars) on MMP-1 expression by fibroblasts and VSMC within 48 h was assessed by ELISA. Each bar represents the mean S.D. of triplicates in six inde- pendent experiments. B, a representative immunoblot demonstrates the role of Erk1/2 and p38 MAP kinase in thehypoxia-inducedexpressionofMMP-1,MMP-13,andhypoxia-induciblefactor-1(HIF-1)aswellasonthe antagonizing effect of PDGF-BB in human lung fibroblasts. PD98059 inhibits Erk1/2 MAP kinase, SB203580 inhibits p38 MAP kinase and SB202474 is a negative control for SB203580. Ab, neutralizing antibody; control t0, protein extract from untreated cells before stimulation. C, hypoxia- and PDGF-BB-induced MMP-13 expression by fibroblasts but not by VSMC within 48 h. Each bar represents the mean S.D. of triplicate determinations from six independent experiments. Similar results were obtained with two additional cell lines.

Article Snippet: The effect of the MMP inhibitor OO-Hy (catalog number 44244; Calbiochem, Lucern, Switzerland) and of neutralizing polyclonal anti-PDGF-BB antibodies (AB-220-NA; R & D Systems) on proliferation (48 h) was also investigated.

Techniques: Expressing, Enzyme-linked Immunosorbent Assay, Western Blot, Negative Control, Control

FIGURE 4. A, the effect of hypoxia and PDGF-BB on the release of soluble collagen type I within 48 h was determined by ELISA in the cell culture medium obtained from human lung fibroblasts (n 6). Each bar represents the mean S.D. Experiments in each cell line were performed in triplicates. B, the inducing effect of hypoxia and PDGF-BB on collagen type I 1 chain (COL1A1) was confirmed on the mRNA level in three cell lines and the effect of Erk1/2 and p38 MAP kinase was assessed by reverse transcriptase-PCR. -ac- tin gene expression was used as a housekeeping gene, and data are displayed as a representative PCR product analysis. PD98059 inhibits Erk1/2 MAP kinase,SB203580inhibitsp38MAPkinase,andSB202474isanegativecontrol for SB203580.

Journal: Journal of Biological Chemistry

Article Title: Cell Type-specific Effect of Hypoxia and Platelet-derived Growth Factor-BB on Extracellular Matrix Turnover and Its Consequences for Lung Remodeling

doi: 10.1074/jbc.m602178200

Figure Lengend Snippet: FIGURE 4. A, the effect of hypoxia and PDGF-BB on the release of soluble collagen type I within 48 h was determined by ELISA in the cell culture medium obtained from human lung fibroblasts (n 6). Each bar represents the mean S.D. Experiments in each cell line were performed in triplicates. B, the inducing effect of hypoxia and PDGF-BB on collagen type I 1 chain (COL1A1) was confirmed on the mRNA level in three cell lines and the effect of Erk1/2 and p38 MAP kinase was assessed by reverse transcriptase-PCR. -ac- tin gene expression was used as a housekeeping gene, and data are displayed as a representative PCR product analysis. PD98059 inhibits Erk1/2 MAP kinase,SB203580inhibitsp38MAPkinase,andSB202474isanegativecontrol for SB203580.

Article Snippet: The effect of the MMP inhibitor OO-Hy (catalog number 44244; Calbiochem, Lucern, Switzerland) and of neutralizing polyclonal anti-PDGF-BB antibodies (AB-220-NA; R & D Systems) on proliferation (48 h) was also investigated.

Techniques: Enzyme-linked Immunosorbent Assay, Cell Culture, Reverse Transcription, Gene Expression

FIGURE 5. A, hypoxia and/or PDGF-BB increased fibroblast numbers significantly within 72 h. Under hypoxic culture condition, PDGF-BB mediates the mitogenic effect that is transmitted via Erk1/2. PD98059 inhibits Erk1/2 MAP kinase, SB203580 inhibits p38 MAP kinase, and SB202474 is a negative control for SB203580. The general inhibitor of MMP activity cis-9-octadecenoyl-N-hydroxylamide oleoyl-N-hydroxylamide (10 mM, OO-Hy) was added 3 h before cells were exposed to any other stimulus. B, hypoxia (black bar) and PDGF-BB up-regulated VSMC proliferation via Erk1/2 and p38 MAP kinase. Purified human soluble collagen type 1 (sol collagen type I (1 mg/ml)) was used to assess its contribution to hypoxia-mediated proliferation. Each bar represents the mean S.D. of triplicates performed in six independent experiments.

Journal: Journal of Biological Chemistry

Article Title: Cell Type-specific Effect of Hypoxia and Platelet-derived Growth Factor-BB on Extracellular Matrix Turnover and Its Consequences for Lung Remodeling

doi: 10.1074/jbc.m602178200

Figure Lengend Snippet: FIGURE 5. A, hypoxia and/or PDGF-BB increased fibroblast numbers significantly within 72 h. Under hypoxic culture condition, PDGF-BB mediates the mitogenic effect that is transmitted via Erk1/2. PD98059 inhibits Erk1/2 MAP kinase, SB203580 inhibits p38 MAP kinase, and SB202474 is a negative control for SB203580. The general inhibitor of MMP activity cis-9-octadecenoyl-N-hydroxylamide oleoyl-N-hydroxylamide (10 mM, OO-Hy) was added 3 h before cells were exposed to any other stimulus. B, hypoxia (black bar) and PDGF-BB up-regulated VSMC proliferation via Erk1/2 and p38 MAP kinase. Purified human soluble collagen type 1 (sol collagen type I (1 mg/ml)) was used to assess its contribution to hypoxia-mediated proliferation. Each bar represents the mean S.D. of triplicates performed in six independent experiments.

Article Snippet: The effect of the MMP inhibitor OO-Hy (catalog number 44244; Calbiochem, Lucern, Switzerland) and of neutralizing polyclonal anti-PDGF-BB antibodies (AB-220-NA; R & D Systems) on proliferation (48 h) was also investigated.

Techniques: Negative Control, Activity Assay, Purification