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Image Search Results
Journal: Frontiers in Oncology
Article Title: LOXL2 Expression Status Is Correlated With Molecular Characterizations of Cervical Carcinoma and Associated With Poor Cancer Survival via Epithelial-Mesenchymal Transition (EMT) Phenotype
doi: 10.3389/fonc.2020.00284
Figure Lengend Snippet: The expression of APOBEC3 family genes negatively correlated with the LOXL2 status in cervical cancer. (A) Scatter plots of APOBEC3 family mRNA expression in the two groups clustered by LOXL2 expression in 176 core-set cervical cancer samples. Y axis shows the log2 of (RSEM (RNA-Seq by Expectation-Maximization)+1). Mean and SD were shown. P- values were calculated by Student's t -test. (B) qPCR of APOBEC3 family genes in SiHa from CON and sh-LOXL2 ( n = 3). Mean and SEM were shown. P- values were calculated by Student's t -test. (* <0.05, ** <0.01) (C) Pearson correlation analysis of APOBEC3 family genes as APOBEC3A, APOBEC3B, APOBEC3D, and APOBEC3G with that of LOXL2 expression in TCGA dataset from GEPIA . P- value and Pearson correlation coefficient (r) were shown. (D) Representative images of IF assay in SiHa and HeLa cell lines. (E) Western blot assay of LOXL2, APOBEC3A, APOBEC3B, APOBEC3D, and APOBEC3G in SiHa and HeLa after transfected with sh-Con or sh-LOXL2. β-tubulin served as the loading control.
Article Snippet: The PVDF membrane was incubated with diluted GAPDH (A10471, ABclonal, 1:2000), LOXL2 antibody (A4708, ABclonal, 1:1000), SNAI1 antibody (A12301, ABclonal,1:1000), E-Cadherin (GTX100443, 1:1000), N-Cadherin (A10206, ABclonal, 1:1000), Vimentin (3932, CST,1:1000), APOBEC3A (A12399, ABclonal, 1:400), APOBEC3B (A9010, ABclonal, 1:1000), APOBEC3D (A11648, ABclonal, 1:1000),
Techniques: Expressing, RNA Sequencing Assay, Western Blot, Transfection