Journal: Experimental and Therapeutic Medicine

Article Title: BMP14 induces tenogenic differentiation of bone marrow mesenchymal stem cells in vitro

doi: 10.3892/etm.2018.6293

Figure Lengend Snippet: Characterization of BMSCs. (A) Representative images show immunofluorescent staining of CD29 and CD44 on passage 2 BMSCs. Scale bars, 50 µm. Pink, CD29; green, CD44; blue, DAPI. (B) Flow cytometry identified rat BMSC-positive markers CD44, CD90 and CD73, but not the marker CD45 on the surface of the cells. CD, cluster of differentiation; BMSC, bone marrow mesenchymal stem cell.

Article Snippet: CD44H monoclonal antibody (12-0444-82), CD73 monoclonal antibody (11-0739-42), CD90.1 Monoclonal antibody (A16370) and CD45 monoclonal antibody (11-0461-82) were purchased from eBioscience (Thermo Fisher Scientific, Inc.).

Techniques: Staining, Flow Cytometry, Marker

Journal: Cells

Article Title: CircZXDC Promotes Vascular Smooth Muscle Cell Transdifferentiation via Regulating miRNA-125a-3p/ABCC6 in Moyamoya Disease

doi: 10.3390/cells11233792

Figure Lengend Snippet: CircZXDC overexpression results in VSMC transdifferentiation towards the synthetic phenotype. The results of the CCK-8 assay ( A ), ki-67 immunofluorescence staining, scale bar = 100 µm ( B ), Transwell assay, scale bar = 50 µm ( C ), and the wound-healing assay ( D ), measuring the VSMCs transferred with the vector plasmid (Vector) and circZXDC overexpression plasmid (circOE) under non-OGD/OGD conditions; ( E ) Western blot analysis of the protein expression of OPN, VIM, and EREG in the VSMCs transferred with the vector plasmid (Vector) and the circZXDC overexpression plasmid (circOE) under non-OGD/OGD conditions. The results of all the groups are shown as mean ± SD; two-way ANOVA with Tukey’s post hoc test was used. The significance level was accepted as * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001.

Article Snippet: The membranes were blocked with NcmBlot blocking buffer (NCM Biotech, Suzhou, China) and then incubated with the following primary antibodies overnight at 4 °C: anti-ABCC6 (27848-1-AP, 1:1000, Proteintech, Wuhan, China), anti-osteopontin (OPN, 22952-1-AP, 1:1000, Proteintech, Wuhan, China), anti-vimentin (VIM, 10366-1-AP, 1:1000, Proteintech, Wuhan, China), anti-EREG (A16372, 1:1000, ABclonal Biotech Co., Ltd., Shanghai, China), anti-xbp1 ( 24868-1-AP, 1:1000, Proteintech, Wuhan, China), anti-GRP78 ( 11587-1-AP, 1:1000, Proteintech, Wuhan, China), anti-GAPDH (A19056, 1:2000, ABclonal Biotech Co., Ltd., Shanghai, China), anti-β-actin (AC026, 1:2000, ABclonal Biotech Co., Ltd., Shanghai, China), and b-tubulin (GB11017, 1:1000, Wuhan Servicebio Technology Co., Ltd., Wuhan, China).

Techniques: Over Expression, CCK-8 Assay, Immunofluorescence, Staining, Transwell Assay, Wound Healing Assay, Plasmid Preparation, Western Blot, Expressing

Journal: Mediators of Inflammation

Article Title: How Adhesion Molecule Patterns Change While Neutrophils Traffic through the Lung during Inflammation

doi: 10.1155/2019/1208086

Figure Lengend Snippet: Impact of inflammation on the expression of CD31 (a), CD54 (b), and CD162 (c) on PMNs in the different compartments of the lung. All mice inhaled LPS and the fraction of PMNs which express the particular adhesion molecule on their surface was determined (% of frequency of parents) in the different compartments of the lung (IV = intravascular; ADHERENT = adherent to the endothelium; IS = interstitial; BAL = bronchoalveolar lavage). Additionally, the mean fluorescence intensity (MFI) was analyzed for the PMNs in each compartment and one representative histogram is shown, whereas the red histogram resembles the negative control. Data are presented as mean ± SD; n = 8 − 12 (a–c); ∗ P < 0.05; ∗∗ P < 0.01; ∗∗∗ P < 0.001 as indicated.

Article Snippet: For the detection of the adhesion molecules, the following specific fluorescent antibodies were added to cell suspensions: CD11a (catalog number 553121, BD, Heidelberg, Germany), CD11b (553311, BD), CD31 (553373, BD), CD54 (A16333, Life Technologies, Frederick, USA), CD162 (555306, BD), CD47 (563585, BD), CD172a (144011, BioLegend, San Diego, CA, USA), CD44 (553134, BD), and CD29 (25-0291-80 eBioscience, San Diego, USA).

Techniques: Expressing, Fluorescence, Negative Control

Journal: Cell Death Discovery

Article Title: Upregulation of rate-limiting enzymes in cholesterol metabolism by PKCδ mediates endothelial apoptosis in diabetic wound healing

doi: 10.1038/s41420-024-02030-2

Figure Lengend Snippet: A ACAT2; B HMGCS1; C HMGCR; D MVK; E PMVK; F MVD; G IDI1; H FDPS; I FDFT1; J SQLE; K LSS. Genes colored green were differentially expressed in two groups and selected for subsequent detection. Quantification was done using the Dr. TOM platform. The results are presented as the mean ± SEM, * q < 0.05 vs controls; ns not significant.

Article Snippet: The antibodies used in this study included: PKCδ polyclonal antibody (#19132-1-AP, Proteintech), Caspase 3/p17/p19 Polyclonal antibody (#19677-1-AP, Proteintech), HMGCS1 Rabbit pAb (#A3916, ABclonal Techonlogy), HMGCR Rabbit pAb (#A1633, ABclonal Techonlogy), GAPDH Monoclonal antibody (#60004-1-Ig, Proteintech), Beta Actin Monoclonal antibody (#66009-1-Ig, Proteintech), HRP-conjugated Affinipure Goat Anti-Rabbit IgG(H + L) (#SA00001-2, proteintech), HRP-conjugated Affinipure Goat Anti-Mouse IgG(H + L) (#SA00001-1, Proteintech), CoraLite488-conjugated Goat Anti-Rabbit IgG(H + L) (#SA00013-2, Proteintech).

Techniques:

Journal: Cell Death Discovery

Article Title: Upregulation of rate-limiting enzymes in cholesterol metabolism by PKCδ mediates endothelial apoptosis in diabetic wound healing

doi: 10.1038/s41420-024-02030-2

Figure Lengend Snippet: A , B qPCR analysis of the mRNA level of HMGCS1 and HMGCR after high glucose treatment for 48 h. C – F Western blot and quantification of the protein level of HMGCS1 and HMGCR after high glucose treatment for 72 h. G – J Immunofluorescence of HMGCS1 and HMGCR and quantification of fluorescence intensity after high glucose treatment for 72 h. K Free cholesterol assay 72 h after high glucose treatment. Each experiment was replicated at least thrice, and representative images were shown. Quantification was done using a two-tailed unpaired Student’s t test. The results are presented as the mean ± SEM, * p < 0.05, ** p < 0.01, **** p < 0.0001 vs controls.

Article Snippet: The antibodies used in this study included: PKCδ polyclonal antibody (#19132-1-AP, Proteintech), Caspase 3/p17/p19 Polyclonal antibody (#19677-1-AP, Proteintech), HMGCS1 Rabbit pAb (#A3916, ABclonal Techonlogy), HMGCR Rabbit pAb (#A1633, ABclonal Techonlogy), GAPDH Monoclonal antibody (#60004-1-Ig, Proteintech), Beta Actin Monoclonal antibody (#66009-1-Ig, Proteintech), HRP-conjugated Affinipure Goat Anti-Rabbit IgG(H + L) (#SA00001-2, proteintech), HRP-conjugated Affinipure Goat Anti-Mouse IgG(H + L) (#SA00001-1, Proteintech), CoraLite488-conjugated Goat Anti-Rabbit IgG(H + L) (#SA00013-2, Proteintech).

Techniques: Western Blot, Immunofluorescence, Fluorescence, Cholesterol Assay, Two Tailed Test

Journal: Cell Death Discovery

Article Title: Upregulation of rate-limiting enzymes in cholesterol metabolism by PKCδ mediates endothelial apoptosis in diabetic wound healing

doi: 10.1038/s41420-024-02030-2

Figure Lengend Snippet: A , B qPCR analysis of the mRNA level of HMGCS1 and HMGCR after transfection for 72 h. C – F Western blot and quantification of the protein level of HMGCS1 and HMGCR 72 h after transfection. G – J Immunofluorescence of HMGCS1 and HMGCR and quantification of fluorescence intensity after transfection for 72 h. K Free cholesterol assay 72 h after transfection. Each experiment was replicated at least thrice and representative images were shown. Quantification was done using a two-tailed unpaired Student’s t test. The results are presented as the mean ± SEM, * p < 0.05, ** p < 0.01, *** p < 0.001 vs controls.

Article Snippet: The antibodies used in this study included: PKCδ polyclonal antibody (#19132-1-AP, Proteintech), Caspase 3/p17/p19 Polyclonal antibody (#19677-1-AP, Proteintech), HMGCS1 Rabbit pAb (#A3916, ABclonal Techonlogy), HMGCR Rabbit pAb (#A1633, ABclonal Techonlogy), GAPDH Monoclonal antibody (#60004-1-Ig, Proteintech), Beta Actin Monoclonal antibody (#66009-1-Ig, Proteintech), HRP-conjugated Affinipure Goat Anti-Rabbit IgG(H + L) (#SA00001-2, proteintech), HRP-conjugated Affinipure Goat Anti-Mouse IgG(H + L) (#SA00001-1, Proteintech), CoraLite488-conjugated Goat Anti-Rabbit IgG(H + L) (#SA00013-2, Proteintech).

Techniques: Transfection, Western Blot, Immunofluorescence, Fluorescence, Cholesterol Assay, Two Tailed Test

Journal: International Journal of Molecular Sciences

Article Title: ADCY5 Gene Affects Seasonal Reproduction in Dairy Goats by Regulating Ovarian Granulosa Cells Steroid Hormone Synthesis

doi: 10.3390/ijms26041622

Figure Lengend Snippet: The antibody information.

Article Snippet: CYP11A1 , A16363 , Abclonal , 1:1000.

Techniques: